ID   UBE2F_BOVIN             Reviewed;         185 AA.
AC   Q1RMW1;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2006, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=NEDD8-conjugating enzyme UBE2F;
DE            EC=2.3.2.32 {ECO:0000250|UniProtKB:Q969M7};
DE   AltName: Full=NEDD8 carrier protein UBE2F;
DE   AltName: Full=NEDD8 protein ligase UBE2F;
DE   AltName: Full=NEDD8-conjugating enzyme 2;
DE   AltName: Full=RING-type E3 NEDD8 transferase UBE2F;
DE   AltName: Full=Ubiquitin-conjugating enzyme E2 F;
GN   Name=UBE2F;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Uterus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1
CC       E1 complex and catalyzes its covalent attachment to other proteins. The
CC       specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1,
CC       suggests that the RBX2-UBE2F complex neddylates specific target
CC       proteins, such as CUL5. {ECO:0000250|UniProtKB:Q969M7}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine
CC         + [cullin]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine +
CC         N(6)-[NEDD8-protein]-yl-[cullin]-L-lysine.; EC=2.3.2.32;
CC         Evidence={ECO:0000250|UniProtKB:Q969M7};
CC   -!- PATHWAY: Protein modification; protein neddylation.
CC   -!- SUBUNIT: Interacts with UBA3 and RBX2. Interacts (N-terminally
CC       acetylated form) with (via DCUN1 domain) DCUN1D1, DCUN1D2, DCUN1D3,
CC       DCUN1D4 and DCUN1D5 (By similarity). {ECO:0000250|UniProtKB:Q969M7}.
CC   -!- PTM: The acetylation of Met-1 increases affinity for DCUN1D3 by about 2
CC       orders of magnitude and is crucial for NEDD8 transfer to cullins.
CC       {ECO:0000250|UniProtKB:Q969M7}.
CC   -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. UBE2F
CC       subfamily. {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR   EMBL; BC114675; AAI14676.1; -; mRNA.
DR   RefSeq; NP_001069911.1; NM_001076443.1.
DR   AlphaFoldDB; Q1RMW1; -.
DR   SMR; Q1RMW1; -.
DR   STRING; 9913.ENSBTAP00000036864; -.
DR   PaxDb; Q1RMW1; -.
DR   Ensembl; ENSBTAT00000037020; ENSBTAP00000036864; ENSBTAG00000026114.
DR   GeneID; 617083; -.
DR   KEGG; bta:617083; -.
DR   CTD; 140739; -.
DR   VEuPathDB; HostDB:ENSBTAG00000026114; -.
DR   VGNC; VGNC:55693; UBE2F.
DR   eggNOG; KOG0420; Eukaryota.
DR   GeneTree; ENSGT00940000154349; -.
DR   HOGENOM; CLU_030988_6_4_1; -.
DR   InParanoid; Q1RMW1; -.
DR   OMA; YNMAPPK; -.
DR   OrthoDB; 1302735at2759; -.
DR   TreeFam; TF101125; -.
DR   Reactome; R-BTA-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR   UniPathway; UPA00885; -.
DR   Proteomes; UP000009136; Chromosome 3.
DR   Bgee; ENSBTAG00000026114; Expressed in pons and 108 other tissues.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0061654; F:NEDD8 conjugating enzyme activity; ISS:UniProtKB.
DR   GO; GO:0019788; F:NEDD8 transferase activity; IBA:GO_Central.
DR   GO; GO:0045116; P:protein neddylation; ISS:UniProtKB.
DR   CDD; cd00195; UBCc; 1.
DR   Gene3D; 3.10.110.10; -; 1.
DR   InterPro; IPR000608; UBQ-conjugat_E2.
DR   InterPro; IPR023313; UBQ-conjugating_AS.
DR   InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR   Pfam; PF00179; UQ_con; 1.
DR   SUPFAM; SSF54495; SSF54495; 1.
DR   PROSITE; PS00183; UBC_1; 1.
DR   PROSITE; PS50127; UBC_2; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; ATP-binding; Nucleotide-binding; Reference proteome;
KW   Transferase; Ubl conjugation pathway.
FT   CHAIN           1..185
FT                   /note="NEDD8-conjugating enzyme UBE2F"
FT                   /id="PRO_0000263076"
FT   DOMAIN          32..185
FT                   /note="UBC core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT   REGION          1..29
FT                   /note="Interaction with UBA3"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        116
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT                   ECO:0000255|PROSITE-ProRule:PRU10133"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0000250|UniProtKB:Q969M7"
SQ   SEQUENCE   185 AA;  21133 MW;  5DF8D78075B520F4 CRC64;
     MLTLASKLKR DDGLKGSRAS ATASDSTRRV SVRDRLLVKE VAELEANLPC TCKVHFPDPN
     KLHCFQLTVT PDEGYYQGGK FQFETEVPDA YNMVPPKVKC LTRIWHPNIT ETGEICLSLL
     REHSIDGTGW APTRTLKDVV WGLNSLFTDL LNFDDPLNIE AAEHHLRDKE DFRNKVEDYI
     KRYAR