ID   UBE2F_CHICK             Reviewed;         185 AA.
AC   Q5ZKX6;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=NEDD8-conjugating enzyme UBE2F;
DE            EC=2.3.2.32 {ECO:0000250|UniProtKB:Q969M7};
DE   AltName: Full=NEDD8 carrier protein UBE2F;
DE   AltName: Full=NEDD8 protein ligase UBE2F;
DE   AltName: Full=NEDD8-conjugating enzyme 2;
DE   AltName: Full=RING-type E3 NEDD8 transferase UBE2F;
DE   AltName: Full=Ubiquitin-conjugating enzyme E2 F;
GN   Name=UBE2F; ORFNames=RCJMB04_8m10;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=CB; TISSUE=Bursa of Fabricius;
RX   PubMed=15642098; DOI=10.1186/gb-2004-6-1-r6;
RA   Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P.,
RA   Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P.,
RA   Hayashizaki Y., Buerstedde J.-M.;
RT   "Full-length cDNAs from chicken bursal lymphocytes to facilitate gene
RT   function analysis.";
RL   Genome Biol. 6:R6.1-R6.9(2005).
CC   -!- FUNCTION: Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1
CC       E1 complex and catalyzes its covalent attachment to other proteins. The
CC       specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1,
CC       suggests that the RBX2-UBE2F complex neddylates specific target
CC       proteins, such as CUL5. {ECO:0000250|UniProtKB:Q969M7}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine
CC         + [cullin]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine +
CC         N(6)-[NEDD8-protein]-yl-[cullin]-L-lysine.; EC=2.3.2.32;
CC         Evidence={ECO:0000250|UniProtKB:Q969M7};
CC   -!- PATHWAY: Protein modification; protein neddylation.
CC   -!- SUBUNIT: Interacts with UBA3 and RBX2. {ECO:0000250|UniProtKB:Q969M7}.
CC   -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. UBE2F
CC       subfamily. {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR   EMBL; AJ719958; CAG31617.1; -; mRNA.
DR   RefSeq; NP_001006512.1; NM_001006512.1.
DR   AlphaFoldDB; Q5ZKX6; -.
DR   SMR; Q5ZKX6; -.
DR   PaxDb; Q5ZKX6; -.
DR   Ensembl; ENSGALT00000006045; ENSGALP00000006035; ENSGALG00000003812.
DR   Ensembl; ENSGALT00000054996; ENSGALP00000053500; ENSGALG00000003812.
DR   Ensembl; ENSGALT00000056310; ENSGALP00000052225; ENSGALG00000003812.
DR   GeneID; 424015; -.
DR   KEGG; gga:424015; -.
DR   CTD; 140739; -.
DR   VEuPathDB; HostDB:geneid_424015; -.
DR   GeneTree; ENSGT00940000154349; -.
DR   InParanoid; Q5ZKX6; -.
DR   OMA; YNMAPPK; -.
DR   PhylomeDB; Q5ZKX6; -.
DR   UniPathway; UPA00885; -.
DR   PRO; PR:Q5ZKX6; -.
DR   Proteomes; UP000000539; Chromosome 7.
DR   Bgee; ENSGALG00000003812; Expressed in muscle tissue and 14 other tissues.
DR   ExpressionAtlas; Q5ZKX6; baseline.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0061654; F:NEDD8 conjugating enzyme activity; ISS:UniProtKB.
DR   GO; GO:0019788; F:NEDD8 transferase activity; IBA:GO_Central.
DR   GO; GO:0045116; P:protein neddylation; ISS:UniProtKB.
DR   CDD; cd00195; UBCc; 1.
DR   Gene3D; 3.10.110.10; -; 1.
DR   InterPro; IPR000608; UBQ-conjugat_E2.
DR   InterPro; IPR023313; UBQ-conjugating_AS.
DR   InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR   Pfam; PF00179; UQ_con; 1.
DR   SUPFAM; SSF54495; SSF54495; 1.
DR   PROSITE; PS00183; UBC_1; 1.
DR   PROSITE; PS50127; UBC_2; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Nucleotide-binding; Reference proteome; Transferase;
KW   Ubl conjugation pathway.
FT   CHAIN           1..185
FT                   /note="NEDD8-conjugating enzyme UBE2F"
FT                   /id="PRO_0000263079"
FT   DOMAIN          32..185
FT                   /note="UBC core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT   REGION          1..29
FT                   /note="Interaction with UBA3"
FT                   /evidence="ECO:0000250"
FT   REGION          11..30
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        116
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT                   ECO:0000255|PROSITE-ProRule:PRU10133"
SQ   SEQUENCE   185 AA;  21229 MW;  2351380A0C29FA40 CRC64;
     MLTLASKLKR DDGVRGPRAS NPASDSTRRV SVRDKLLVKE VAELEANLPC TCKVNFPDPN
     KLHYFQLTVI PDEGYYQGGK FQFEIEVPDA YNMVPPKVKC LTRIWHPNIT ETGEICLSLL
     REHSIDGTGW APTRTLKDVV WGLNSLFTDL LNFDDPLNIE AAEHHLRDKE DFRNKVEDYI
     KRYAR