UBE2F_DANRE
ID UBE2F_DANRE Reviewed; 185 AA.
AC Q6NY82; B7ZD57; B7ZD58;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=NEDD8-conjugating enzyme UBE2F;
DE EC=2.3.2.32 {ECO:0000250|UniProtKB:Q969M7};
DE AltName: Full=NEDD8 carrier protein UBE2F;
DE AltName: Full=NEDD8 protein ligase UBE2F;
DE AltName: Full=NEDD8-conjugating enzyme 2;
DE AltName: Full=RING-type E3 NEDD8 transferase UBE2F;
DE AltName: Full=Ubiquitin-conjugating enzyme E2 F;
GN Name=ube2f; ORFNames=ch211-93g23.1, zgc:77005;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Accepts the ubiquitin-like protein NEDD8 from the uba3-nae1
CC E1 complex and catalyzes its covalent attachment to other proteins. The
CC specific interaction with the E3 ubiquitin ligase rbx2, but not rbx1,
CC suggests that the rbx2-ube2f complex neddylates specific target
CC proteins, such as cul5. {ECO:0000250|UniProtKB:Q969M7}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine
CC + [cullin]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine +
CC N(6)-[NEDD8-protein]-yl-[cullin]-L-lysine.; EC=2.3.2.32;
CC Evidence={ECO:0000250|UniProtKB:Q969M7};
CC -!- PATHWAY: Protein modification; protein neddylation.
CC -!- SUBUNIT: Interacts with uba3 and rbx2. {ECO:0000250|UniProtKB:Q969M7}.
CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. UBE2F
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAX12886.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL929396; CAX12885.1; -; Genomic_DNA.
DR EMBL; AL929396; CAX12886.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BC066702; AAH66702.1; -; mRNA.
DR RefSeq; NP_998479.1; NM_213314.1.
DR RefSeq; XP_005160353.1; XM_005160296.3.
DR RefSeq; XP_009303965.1; XM_009305690.2.
DR AlphaFoldDB; Q6NY82; -.
DR SMR; Q6NY82; -.
DR STRING; 7955.ENSDARP00000094476; -.
DR PaxDb; Q6NY82; -.
DR Ensembl; ENSDART00000103701; ENSDARP00000094476; ENSDARG00000025754.
DR Ensembl; ENSDART00000144641; ENSDARP00000120196; ENSDARG00000025754.
DR GeneID; 406606; -.
DR KEGG; dre:406606; -.
DR CTD; 140739; -.
DR ZFIN; ZDB-GENE-040426-2557; ube2f.
DR eggNOG; KOG0420; Eukaryota.
DR GeneTree; ENSGT00940000154349; -.
DR HOGENOM; CLU_030988_6_4_1; -.
DR InParanoid; Q6NY82; -.
DR OMA; YNMAPPK; -.
DR OrthoDB; 1302735at2759; -.
DR PhylomeDB; Q6NY82; -.
DR TreeFam; TF101125; -.
DR Reactome; R-DRE-8951664; Neddylation.
DR Reactome; R-DRE-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR UniPathway; UPA00885; -.
DR PRO; PR:Q6NY82; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 1.
DR Bgee; ENSDARG00000025754; Expressed in testis and 21 other tissues.
DR ExpressionAtlas; Q6NY82; baseline.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0061654; F:NEDD8 conjugating enzyme activity; ISS:UniProtKB.
DR GO; GO:0019788; F:NEDD8 transferase activity; IBA:GO_Central.
DR GO; GO:0045116; P:protein neddylation; ISS:UniProtKB.
DR CDD; cd00195; UBCc; 1.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR000608; UBQ-conjugat_E2.
DR InterPro; IPR023313; UBQ-conjugating_AS.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR Pfam; PF00179; UQ_con; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS00183; UBC_1; 1.
DR PROSITE; PS50127; UBC_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Nucleotide-binding; Reference proteome; Transferase;
KW Ubl conjugation pathway.
FT CHAIN 1..185
FT /note="NEDD8-conjugating enzyme UBE2F"
FT /id="PRO_0000263080"
FT DOMAIN 32..185
FT /note="UBC core"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT REGION 1..29
FT /note="Interaction with uba3"
FT /evidence="ECO:0000250"
FT ACT_SITE 116
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT ECO:0000255|PROSITE-ProRule:PRU10133"
SQ SEQUENCE 185 AA; 21019 MW; EA6826F38C074814 CRC64;
MLTLASKLKR EEGVRAGRTP AGSNDAAHRV SIRDRLLIKE VAELEANLPS TCKVTFPDEN
KLCHFQLAIS PDEGYYLGGK FQFEIEVPEA YNMVPPKVKC LTRIWHPNIA ETGEICLSLL
REHSIDGTGW APTRTLKDVV WGLNSLFTDL LNFDDPLNID AAEHHLRDKE DFRNKVQDFI
KNYAR
