UBE2F_XENLA
ID UBE2F_XENLA Reviewed; 185 AA.
AC Q6IRC7;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=NEDD8-conjugating enzyme UBE2F;
DE EC=2.3.2.32 {ECO:0000250|UniProtKB:Q969M7};
DE AltName: Full=NEDD8 carrier protein UBE2F;
DE AltName: Full=NEDD8-conjugating enzyme 2;
DE AltName: Full=Ubiquitin-conjugating enzyme E2 F;
GN Name=ube2f;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC TISSUE=Embryo;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Accepts the ubiquitin-like protein NEDD8 from the uba3-nae1
CC E1 complex and catalyzes its covalent attachment to other proteins. The
CC specific interaction with the E3 ubiquitin ligase rbx2, but not rbx1,
CC suggests that the rbx2-ube2f complex neddylates specific target
CC proteins, such as cul5. {ECO:0000250|UniProtKB:Q969M7}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine
CC + [cullin]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine +
CC N(6)-[NEDD8-protein]-yl-[cullin]-L-lysine.; EC=2.3.2.32;
CC Evidence={ECO:0000250|UniProtKB:Q969M7};
CC -!- PATHWAY: Protein modification; protein neddylation.
CC -!- SUBUNIT: Interacts with uba3 and rbx2. {ECO:0000250|UniProtKB:Q969M7}.
CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. UBE2F
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR EMBL; BC070971; AAH70971.1; -; mRNA.
DR RefSeq; NP_001085021.1; NM_001091552.1.
DR AlphaFoldDB; Q6IRC7; -.
DR SMR; Q6IRC7; -.
DR DNASU; 432085; -.
DR GeneID; 432085; -.
DR KEGG; xla:432085; -.
DR CTD; 432085; -.
DR Xenbase; XB-GENE-960082; ube2f.S.
DR OMA; YNMAPPK; -.
DR OrthoDB; 1302735at2759; -.
DR UniPathway; UPA00885; -.
DR Proteomes; UP000186698; Chromosome 9_10S.
DR Bgee; 432085; Expressed in muscle tissue and 20 other tissues.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0061654; F:NEDD8 conjugating enzyme activity; ISS:UniProtKB.
DR GO; GO:0045116; P:protein neddylation; ISS:UniProtKB.
DR CDD; cd00195; UBCc; 1.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR000608; UBQ-conjugat_E2.
DR InterPro; IPR023313; UBQ-conjugating_AS.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR Pfam; PF00179; UQ_con; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS00183; UBC_1; 1.
DR PROSITE; PS50127; UBC_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Nucleotide-binding; Reference proteome; Transferase;
KW Ubl conjugation pathway.
FT CHAIN 1..185
FT /note="NEDD8-conjugating enzyme UBE2F"
FT /id="PRO_0000263081"
FT DOMAIN 32..185
FT /note="UBC core"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT REGION 1..29
FT /note="Interaction with uba3"
FT /evidence="ECO:0000250"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 116
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT ECO:0000255|PROSITE-ProRule:PRU10133"
SQ SEQUENCE 185 AA; 21397 MW; 95490A6AC1E82973 CRC64;
MLTLASKLKR DDGVKGSRTT STTLDSMRRI SVRDRLLVKE VAELEANLPC TCKVNFPDPN
KLHYFHLTVS PDESYYQGGR FQFEIEVPDA YNMVPPKVKC LTRIWHPNIT ETGEICLSLL
REHSIDGTGW APTRTLKDVV WGLNSLFTDL LNFDDPLNIE AAEHHLRDKD EYRNKVEDYI
KRYAR
