ID   UBE2F_XENTR             Reviewed;         185 AA.
AC   Q5M8Y2;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=NEDD8-conjugating enzyme UBE2F;
DE            EC=2.3.2.32 {ECO:0000250|UniProtKB:Q969M7};
DE   AltName: Full=NEDD8 carrier protein UBE2F;
DE   AltName: Full=NEDD8 protein ligase UBE2F;
DE   AltName: Full=NEDD8-conjugating enzyme 2;
DE   AltName: Full=RING-type E3 NEDD8 transferase UBE2F;
DE   AltName: Full=Ubiquitin-conjugating enzyme E2 F;
GN   Name=ube2f; ORFNames=TTpA011n20.1;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Tadpole;
RG   Sanger Xenopus tropicalis EST/cDNA project;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Accepts the ubiquitin-like protein NEDD8 from the uba3-nae1
CC       E1 complex and catalyzes its covalent attachment to other proteins. The
CC       specific interaction with the E3 ubiquitin ligase rbx2, but not rbx1,
CC       suggests that the rbx2-ube2f complex neddylates specific target
CC       proteins, such as cul5. {ECO:0000250|UniProtKB:Q969M7}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine
CC         + [cullin]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine +
CC         N(6)-[NEDD8-protein]-yl-[cullin]-L-lysine.; EC=2.3.2.32;
CC         Evidence={ECO:0000250|UniProtKB:Q969M7};
CC   -!- PATHWAY: Protein modification; protein neddylation.
CC   -!- SUBUNIT: Interacts with uba3 and rbx2. {ECO:0000250|UniProtKB:Q969M7}.
CC   -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. UBE2F
CC       subfamily. {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR   EMBL; CR848249; CAJ82970.1; -; mRNA.
DR   EMBL; BC087785; AAH87785.1; -; mRNA.
DR   RefSeq; NP_001011221.1; NM_001011221.1.
DR   AlphaFoldDB; Q5M8Y2; -.
DR   SMR; Q5M8Y2; -.
DR   STRING; 8364.ENSXETP00000023831; -.
DR   PaxDb; Q5M8Y2; -.
DR   DNASU; 496657; -.
DR   Ensembl; ENSXETT00000023831; ENSXETP00000023831; ENSXETG00000010905.
DR   GeneID; 496657; -.
DR   KEGG; xtr:496657; -.
DR   CTD; 140739; -.
DR   Xenbase; XB-GENE-960076; ube2f.
DR   eggNOG; KOG0420; Eukaryota.
DR   HOGENOM; CLU_030988_6_4_1; -.
DR   InParanoid; Q5M8Y2; -.
DR   OrthoDB; 1302735at2759; -.
DR   Reactome; R-XTR-8951664; Neddylation.
DR   Reactome; R-XTR-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR   UniPathway; UPA00885; -.
DR   Proteomes; UP000008143; Chromosome 9.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000010905; Expressed in skeletal muscle tissue and 14 other tissues.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0061654; F:NEDD8 conjugating enzyme activity; ISS:UniProtKB.
DR   GO; GO:0019788; F:NEDD8 transferase activity; IBA:GO_Central.
DR   GO; GO:0045116; P:protein neddylation; ISS:UniProtKB.
DR   CDD; cd00195; UBCc; 1.
DR   Gene3D; 3.10.110.10; -; 1.
DR   InterPro; IPR000608; UBQ-conjugat_E2.
DR   InterPro; IPR023313; UBQ-conjugating_AS.
DR   InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR   Pfam; PF00179; UQ_con; 1.
DR   SUPFAM; SSF54495; SSF54495; 1.
DR   PROSITE; PS00183; UBC_1; 1.
DR   PROSITE; PS50127; UBC_2; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Nucleotide-binding; Reference proteome; Transferase;
KW   Ubl conjugation pathway.
FT   CHAIN           1..185
FT                   /note="NEDD8-conjugating enzyme UBE2F"
FT                   /id="PRO_0000263082"
FT   DOMAIN          32..185
FT                   /note="UBC core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT   REGION          1..29
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1..29
FT                   /note="Interaction with uba3"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        116
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT                   ECO:0000255|PROSITE-ProRule:PRU10133"
SQ   SEQUENCE   185 AA;  21313 MW;  CBE6BEE003823EF6 CRC64;
     MLTLASKLKR DDGVKGSRTS STTSDSTRRV SVRDRLLVKE VAELEANLPC TCKVNFPDPN
     KLHYFHLTVS PDESYYQGGR FQFEIEVPDA YNMVPPKVKC LTRIWHPNIT ETGEICLSLL
     REHSIDGTGW APTRTLKDVV WGLNSLFTDL LNFDDPLNIE AAEHHLRDKD EYRNKVEDYI
     KRYAR