UBE2H_BOVIN
ID UBE2H_BOVIN Reviewed; 183 AA.
AC Q32LN1;
DT 11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Ubiquitin-conjugating enzyme E2 H;
DE EC=2.3.2.23 {ECO:0000250|UniProtKB:P62256};
DE AltName: Full=(E3-independent) E2 ubiquitin-conjugating enzyme H;
DE EC=2.3.2.24 {ECO:0000250|UniProtKB:P62256};
DE AltName: Full=E2 ubiquitin-conjugating enzyme H;
DE AltName: Full=Ubiquitin carrier protein H;
DE AltName: Full=Ubiquitin-protein ligase H;
GN Name=UBE2H;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Liver;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Accepts ubiquitin from the E1 complex and catalyzes its
CC covalent attachment to other proteins. E2 ubiquitin conjugating enzyme
CC that transfers ubiquitin to MAEA, a core component of the CTLH E3
CC ubiquitin-protein ligase complex. In vitro catalyzes 'Lys-11'- and
CC 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate
CC histone H2A. {ECO:0000250|UniProtKB:P62256}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC Evidence={ECO:0000250|UniProtKB:P62256, ECO:0000255|PROSITE-
CC ProRule:PRU00388, ECO:0000255|PROSITE-ProRule:PRU10133};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-
CC cysteine + N(6)-monoubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.24; Evidence={ECO:0000250|UniProtKB:P62256};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC -!- SUBUNIT: Interacts with MAEA and WDR26, components of the CTLH complex
CC that contains GID4, RANBP9 and/or RANBP10, MKLN1, MAEA, RMND5A (or
CC alternatively its paralog RMND5B), GID8, ARMC8, WDR26 and YPEL5.
CC {ECO:0000250|UniProtKB:P62256}.
CC -!- PTM: Autoubiquitinated in vitro in the presence of NEDD4L.
CC {ECO:0000250|UniProtKB:P62256}.
CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR EMBL; BC109502; AAI09503.1; -; mRNA.
DR RefSeq; NP_001032703.1; NM_001037614.2.
DR AlphaFoldDB; Q32LN1; -.
DR SMR; Q32LN1; -.
DR STRING; 9913.ENSBTAP00000001205; -.
DR PaxDb; Q32LN1; -.
DR PRIDE; Q32LN1; -.
DR Ensembl; ENSBTAT00000001205; ENSBTAP00000001205; ENSBTAG00000000911.
DR GeneID; 539313; -.
DR KEGG; bta:539313; -.
DR CTD; 7328; -.
DR VEuPathDB; HostDB:ENSBTAG00000000911; -.
DR VGNC; VGNC:36583; UBE2H.
DR eggNOG; KOG0416; Eukaryota.
DR GeneTree; ENSGT00390000004852; -.
DR HOGENOM; CLU_030988_7_5_1; -.
DR InParanoid; Q32LN1; -.
DR OMA; EFCVKFY; -.
DR OrthoDB; 1301162at2759; -.
DR TreeFam; TF101121; -.
DR Reactome; R-BTA-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000009136; Chromosome 4.
DR Bgee; ENSBTAG00000000911; Expressed in semen and 105 other tissues.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IBA:GO_Central.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; ISS:UniProtKB.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IEA:Ensembl.
DR GO; GO:0070979; P:protein K11-linked ubiquitination; ISS:UniProtKB.
DR GO; GO:0070936; P:protein K48-linked ubiquitination; ISS:UniProtKB.
DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR CDD; cd00195; UBCc; 1.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR000608; UBQ-conjugat_E2.
DR InterPro; IPR023313; UBQ-conjugating_AS.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR Pfam; PF00179; UQ_con; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS00183; UBC_1; 1.
DR PROSITE; PS50127; UBC_2; 1.
PE 2: Evidence at transcript level;
KW Acetylation; ATP-binding; Nucleotide-binding; Reference proteome;
KW Transferase; Ubl conjugation; Ubl conjugation pathway.
FT CHAIN 1..183
FT /note="Ubiquitin-conjugating enzyme E2 H"
FT /id="PRO_0000245038"
FT DOMAIN 1..150
FT /note="UBC core"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT REGION 152..183
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 87
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT ECO:0000255|PROSITE-ProRule:PRU10133"
FT MOD_RES 60
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:P62257"
SQ SEQUENCE 183 AA; 20655 MW; 3DD5D365945F708C CRC64;
MSSPSPGKRR MDTDVVKLIE SKHEVTILGG LNEFVVKFYG PQGTPYEGGV WKVRVDLPDK
YPFKSPSIGF MNKIFHPNID EASGTVCLDV INQTWTALYD LTNIFESFLP QLLAYPNPID
PLNGDAAAMY LHRPEEYKQK IKEYIQKYAT EEALKEQEEG TGDSSSESSM SDFSEDEAQD
MEL
