ID   UBE2U_MACFA             Reviewed;         322 AA.
AC   Q95LM1;
DT   25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Ubiquitin-conjugating enzyme E2 U;
DE            EC=2.3.2.23;
DE   AltName: Full=E2 ubiquitin-conjugating enzyme U;
DE   AltName: Full=Ubiquitin carrier protein U;
DE   AltName: Full=Ubiquitin-protein ligase U;
GN   Name=UBE2U; ORFNames=QtsA-20104;
OS   Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
OX   NCBI_TaxID=9541;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=12498619; DOI=10.1186/1471-2164-3-36;
RA   Osada N., Hida M., Kusuda J., Tanuma R., Hirata M., Suto Y., Hirai M.,
RA   Terao K., Sugano S., Hashimoto K.;
RT   "Cynomolgus monkey testicular cDNAs for discovery of novel human genes in
RT   the human genome sequence.";
RL   BMC Genomics 3:36-36(2002).
CC   -!- FUNCTION: Catalyzes the covalent attachment of ubiquitin to other
CC       proteins. {ECO:0000250|UniProtKB:Q5VVX9}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC         [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC         activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC         conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU00388, ECO:0000255|PROSITE-
CC         ProRule:PRU10133};
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC   -!- PTM: Autoubiquitinated in vitro in the presence of UBR5.
CC       {ECO:0000250|UniProtKB:Q5VVX9}.
CC   -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR   EMBL; AB072767; BAB69736.1; -; mRNA.
DR   RefSeq; NP_001272035.1; NM_001285106.1.
DR   AlphaFoldDB; Q95LM1; -.
DR   SMR; Q95LM1; -.
DR   STRING; 9541.XP_005543219.1; -.
DR   GeneID; 102126041; -.
DR   CTD; 148581; -.
DR   eggNOG; KOG0419; Eukaryota.
DR   OrthoDB; 1538096at2759; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000233100; Unplaced.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IEA:UniProtKB-EC.
DR   CDD; cd00195; UBCc; 1.
DR   Gene3D; 3.10.110.10; -; 1.
DR   InterPro; IPR000608; UBQ-conjugat_E2.
DR   InterPro; IPR023313; UBQ-conjugating_AS.
DR   InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR   Pfam; PF00179; UQ_con; 1.
DR   SUPFAM; SSF54495; SSF54495; 1.
DR   PROSITE; PS00183; UBC_1; 1.
DR   PROSITE; PS50127; UBC_2; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Nucleotide-binding; Reference proteome; Transferase;
KW   Ubl conjugation; Ubl conjugation pathway.
FT   CHAIN           1..322
FT                   /note="Ubiquitin-conjugating enzyme E2 U"
FT                   /id="PRO_0000082513"
FT   DOMAIN          4..153
FT                   /note="UBC core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT   ACT_SITE        89
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT                   ECO:0000255|PROSITE-ProRule:PRU10133"
SQ   SEQUENCE   322 AA;  37760 MW;  E94D9D0CBF1AA9F2 CRC64;
     MHGRAYLLLQ RDFYDLKENN YKGITARPVS EDMMEWEVEI EGLQNSVWQG LVFQLTIHFT
     SEYNYAPPVV KFVTIPFHPN VDPHTGQPCI DFLDNPKKWN TNYTLSSILL ALQVMLSNPV
     LENPVNLEAA RILTKDESLY RTILKLFNRP LQMKDDSQEL PKDPHKCIGP TKTTSFSDYY
     QAWSRIATSK ATEYYRTALL KDPNFIGQYY KWKKMDLQHH KEWNLKYSVI KCWLARKNRM
     PDEVRHSMEG IKLCPTQIPT TDEIFLESPT AINSVTNIYE TEEEGWKSDT SLYENNTDEP
     WEEEVEDLIS WINTLNTNTL ED