ID   UBE2Z_DANRE             Reviewed;         328 AA.
AC   Q6DG60;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 2.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Ubiquitin-conjugating enzyme E2 Z;
DE            EC=2.3.2.23;
DE   AltName: Full=E2 ubiquitin-conjugating enzyme Z;
DE   AltName: Full=Ubiquitin carrier protein Z;
DE   AltName: Full=Ubiquitin-protein ligase Z;
GN   Name=ube2z; ORFNames=zgc:92419;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the covalent attachment of ubiquitin to other
CC       proteins. May be involved in apoptosis regulation.
CC       {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC         [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC         activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC         conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU00388};
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9H832}. Nucleus
CC       {ECO:0000250|UniProtKB:Q9H832}.
CC   -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAH76491.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; BC076491; AAH76491.1; ALT_INIT; mRNA.
DR   AlphaFoldDB; Q6DG60; -.
DR   SMR; Q6DG60; -.
DR   STRING; 7955.ENSDARP00000128495; -.
DR   PaxDb; Q6DG60; -.
DR   ZFIN; ZDB-GENE-040718-15; ube2z.
DR   eggNOG; KOG0895; Eukaryota.
DR   InParanoid; Q6DG60; -.
DR   Reactome; R-DRE-8866652; Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
DR   Reactome; R-DRE-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:Q6DG60; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IBA:GO_Central.
DR   GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IBA:GO_Central.
DR   GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR   GO; GO:0043066; P:negative regulation of apoptotic process; IBA:GO_Central.
DR   CDD; cd00195; UBCc; 1.
DR   Gene3D; 3.10.110.10; -; 1.
DR   InterPro; IPR000608; UBQ-conjugat_E2.
DR   InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR   Pfam; PF00179; UQ_con; 1.
DR   SUPFAM; SSF54495; SSF54495; 1.
DR   PROSITE; PS50127; UBC_2; 1.
PE   2: Evidence at transcript level;
KW   Apoptosis; ATP-binding; Cytoplasm; Nucleotide-binding; Nucleus;
KW   Reference proteome; Transferase; Ubl conjugation pathway.
FT   CHAIN           1..328
FT                   /note="Ubiquitin-conjugating enzyme E2 Z"
FT                   /id="PRO_0000280518"
FT   DOMAIN          71..225
FT                   /note="UBC core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT   REGION          295..328
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        311..328
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        160
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
SQ   SEQUENCE   328 AA;  36451 MW;  96233C7E09C4D612 CRC64;
     MAHTITPAVE SGLGVLTHAV SSTVPVAVLP SLPPGIGSGV PAGAGLLSQI HATSWDPTLS
     TDWDNEKASQ QCILRIKRDI MSIYKEPPPG MFVVPDPHDM TKIHALITGP FDTPYEGGFF
     LFLFRCPPDY PIHPPRVKLI TTGHNTVRFN PNFYRNGKVC LSILGTWTGP AWSPAQSISS
     VLISIQSLMT ENPYHNEPGF EQERHPGDSK NYNECIRHET MRVAVCDMLE GKVSCPEALW
     SVMEKSFLEY YDFYEGVCKE RLHLQGQNMQ DPFGEKRGRF DYQGLLTRLR AIQRRLREKC
     PPEDNDGDSD SDTSSSGTDP DSQGSSQP