UBE2Z_RAT
ID UBE2Z_RAT Reviewed; 356 AA.
AC Q3B7D1;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 2.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Ubiquitin-conjugating enzyme E2 Z;
DE EC=2.3.2.23;
DE AltName: Full=E2 ubiquitin-conjugating enzyme Z;
DE AltName: Full=Uba6-specific E2 conjugating enzyme 1;
DE Short=Use1;
DE AltName: Full=Ubiquitin carrier protein Z;
DE AltName: Full=Ubiquitin-protein ligase Z;
GN Name=Ube2z;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Prostate;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Catalyzes the covalent attachment of ubiquitin to other
CC proteins. Specific substrate for UBA6, not charged with ubiquitin by
CC UBE1. May be involved in apoptosis regulation. {ECO:0000255|PROSITE-
CC ProRule:PRU00388}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00388};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9H832}. Nucleus
CC {ECO:0000250|UniProtKB:Q9H832}.
CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAI07664.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; BC107663; AAI07664.1; ALT_INIT; mRNA.
DR RefSeq; NP_001032732.2; NM_001037643.2.
DR AlphaFoldDB; Q3B7D1; -.
DR SMR; Q3B7D1; -.
DR STRING; 10116.ENSRNOP00000052365; -.
DR iPTMnet; Q3B7D1; -.
DR PhosphoSitePlus; Q3B7D1; -.
DR jPOST; Q3B7D1; -.
DR PaxDb; Q3B7D1; -.
DR PRIDE; Q3B7D1; -.
DR Ensembl; ENSRNOT00000055500; ENSRNOP00000052365; ENSRNOG00000006868.
DR GeneID; 303478; -.
DR KEGG; rno:303478; -.
DR UCSC; RGD:1308347; rat.
DR CTD; 65264; -.
DR RGD; 1308347; Ube2z.
DR eggNOG; KOG0895; Eukaryota.
DR GeneTree; ENSGT00940000159091; -.
DR HOGENOM; CLU_025097_2_0_1; -.
DR InParanoid; Q3B7D1; -.
DR OMA; ACPEALW; -.
DR OrthoDB; 1404665at2759; -.
DR PhylomeDB; Q3B7D1; -.
DR TreeFam; TF354204; -.
DR Reactome; R-RNO-8866652; Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
DR Reactome; R-RNO-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q3B7D1; -.
DR Proteomes; UP000002494; Chromosome 10.
DR Bgee; ENSRNOG00000006868; Expressed in jejunum and 19 other tissues.
DR Genevisible; Q3B7D1; RN.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISO:RGD.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IBA:GO_Central.
DR GO; GO:0061631; F:ubiquitin conjugating enzyme activity; ISO:RGD.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IBA:GO_Central.
DR GO; GO:0043065; P:positive regulation of apoptotic process; ISO:RGD.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; ISO:RGD.
DR CDD; cd00195; UBCc; 1.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR000608; UBQ-conjugat_E2.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR Pfam; PF00179; UQ_con; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS50127; UBC_2; 1.
PE 1: Evidence at protein level;
KW Apoptosis; ATP-binding; Cytoplasm; Nucleotide-binding; Nucleus;
KW Phosphoprotein; Reference proteome; Transferase; Ubl conjugation pathway.
FT CHAIN 1..356
FT /note="Ubiquitin-conjugating enzyme E2 Z"
FT /id="PRO_0000280517"
FT DOMAIN 101..255
FT /note="UBC core"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 334..356
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 190
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT MOD_RES 339
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q3UE37"
SQ SEQUENCE 356 AA; 38352 MW; 3C77B50527A30676 CRC64;
MAESPTEEAA TATAGAGAAG PGASGVAGVV GVSGSGGGFG PPFLPDVWAA AAAAGGAGGP
GSGLAPLPGL PPSAAAHGAA LLSHWDPTLS SDWDGERTAP QCLLRIKRDI MSIYKEPPPG
MFVVPDTVDM TKIHALITGP FDTPYEGGFF LFVFRCPPDY PIHPPRVKLM TTGNNTVRFN
PNFYRNGKVC LSILGTWTGP AWSPAQSISS VLISIQSLMT ENPYHNEPGF EQERHPGDSK
NYNECIRHET IRVAVCDMME GKCPCPEPLR GVMEKSFLEY YDFYEVACKD RLHLQGQTMQ
DPFGEKRGHF DYQSLLMRLG LIRQKVLERL HNENAEMDSD SSSSGTETDL HGSLRV
