ID   UBE3B_XENTR             Reviewed;        1072 AA.
AC   Q08CZ0;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Ubiquitin-protein ligase E3B;
DE            EC=2.3.2.26;
DE   AltName: Full=HECT-type ubiquitin transferase E3B;
GN   Name=ube3b;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=N6; TISSUE=Oviduct;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: E3 ubiquitin-protein ligase which accepts ubiquitin from an
CC       E2 ubiquitin-conjugating enzyme in the form of a thioester and then
CC       directly transfers the ubiquitin to targeted substrates. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.26;
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
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DR   EMBL; BC124027; AAI24028.1; -; mRNA.
DR   RefSeq; NP_001072875.1; NM_001079407.1.
DR   RefSeq; XP_012821644.1; XM_012966190.2.
DR   AlphaFoldDB; Q08CZ0; -.
DR   SMR; Q08CZ0; -.
DR   STRING; 8364.ENSXETP00000033038; -.
DR   PaxDb; Q08CZ0; -.
DR   DNASU; 780337; -.
DR   GeneID; 780337; -.
DR   KEGG; xtr:780337; -.
DR   CTD; 89910; -.
DR   Xenbase; XB-GENE-1010563; ube3b.
DR   eggNOG; KOG4427; Eukaryota.
DR   HOGENOM; CLU_002173_2_0_1; -.
DR   InParanoid; Q08CZ0; -.
DR   OMA; HRMAHHR; -.
DR   OrthoDB; 1163565at2759; -.
DR   PhylomeDB; Q08CZ0; -.
DR   TreeFam; TF313215; -.
DR   Reactome; R-XTR-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000008143; Chromosome 1.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000018590; Expressed in blastula and 17 other tissues.
DR   ExpressionAtlas; Q08CZ0; baseline.
DR   GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR   GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR   CDD; cd00078; HECTc; 1.
DR   InterPro; IPR044611; E3B/C.
DR   InterPro; IPR000569; HECT_dom.
DR   InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR   InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR   PANTHER; PTHR45700; PTHR45700; 1.
DR   Pfam; PF00632; HECT; 1.
DR   SMART; SM00119; HECTc; 1.
DR   SMART; SM00015; IQ; 1.
DR   SUPFAM; SSF56204; SSF56204; 1.
DR   PROSITE; PS50237; HECT; 1.
DR   PROSITE; PS50096; IQ; 1.
PE   2: Evidence at transcript level;
KW   Reference proteome; Transferase; Ubl conjugation pathway.
FT   CHAIN           1..1072
FT                   /note="Ubiquitin-protein ligase E3B"
FT                   /id="PRO_0000281884"
FT   DOMAIN          29..58
FT                   /note="IQ"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT   DOMAIN          706..1072
FT                   /note="HECT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00104"
FT   ACT_SITE        1040
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00104"
SQ   SEQUENCE   1072 AA;  123086 MW;  42E699E331C54D71 CRC64;
     MFGATQVSKA QFLDKARQAR EERKGLKERD RAAIKIQALI RRFLCRCRLQ KEIRQEVDDF
     LEGTEKGSVK ANALSIFRIS RKLLIIFQIS QDRMRFEKLC RCILNSMEND NEPKVWFVSL
     ALSKDLTLLW IKQIKDILWY CSEYLKQLKP DILQDSKLIT VYLTMLVTFT DTLTWKILKG
     KGESLRPAMN HICANIMGYL NQKGFYSVLQ ILLTNGLARS RPSLSKGTLT ALFSLALRPV
     VAAQYSDNLL RSFLIHIMSV PALVSHLSTL TPDRLSVIES NGLFHKFILF LSREDQCSDV
     CECLEGSHTL CLLGNLIHLG HLTEKVLEEE MFHFVSVLTQ MLSYCQKYVS QKKSNLTHWH
     PVLGWFSQTV DYGLNESMPL LTKQLQHLWG VHMIRNLFRE VLSKKLAENQ DVSVPVSQSS
     SSSPQNNLPM KNLFKRAFQK SASVRHILKP IGGKKVDSPE VQKVCNICVL YQMALTTLTQ
     IRLQILTGLT YLDDLLPKLW AFICELGPQG GLKLFLECLS SDTEESRRLL AMLVLFCDCS
     RHLITILDDI EVYEEQISFK LEELVTISSF LNSFVFKMIW DGLLENAKGE TLELFHSVHG
     WLMVLYERDC RRRFAPEDHW LRKDLKPTLL FQELDKERKR AQLLLQYIPH VIPHKNRVLL
     FRNIVTKEKE KLGLVETNTA SPHVTHITIR RSRMLEDGYD QLRQLSQNAM KGVIRVKFVN
     DLGVDEAGID QDGVFKEFLE EIIKRVFDPA LNLFKTTSSG DRLYPSPTSY IHENYLQLFE
     FVGKMLGKAV YEGIVVDVPF ASFFLSQLLG HHHSIFYSSV DELPSLDSEF YKNLTSIKRY
     DGDISDLGLT LSYDEDVMGQ LVCHELVPGG KSIPVTNENK ISYIHLMAHF RMHTQIKNQT
     VAFISGFRSI IRPEWIRMFS APELQRLISG DNAEIDLDDL KKHTVYYGGF HGSHRVIIWL
     WDILANDFSS EEKAMFLKFV TSCSRPPLLG FAYLKPPFSI RCVEVSDDQD TGDTLGSVLR
     GFFTIRKKEP GGRLPTSSTC FNLLKLPNYS KKGILREKLR YAISMNTGFE LS