ID   UBE3D_PONAB             Reviewed;         389 AA.
AC   Q5RFG8;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 53.
DE   RecName: Full=E3 ubiquitin-protein ligase E3D;
DE            EC=2.3.2.26;
DE   AltName: Full=HECT-type E3 ubiquitin transferase E3D {ECO:0000305};
DE   AltName: Full=UbcH10-binding protein with a HECT-like domain;
DE   AltName: Full=Ubiquitin-conjugating enzyme E2C-binding protein;
GN   Name=UBE3D; Synonyms=H10BH, UBE2CBP;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Heart;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: E3 ubiquitin-protein ligase which accepts ubiquitin from
CC       specific E2 ubiquitin-conjugating enzymes, and transfers it to
CC       substrates, generally promoting their degradation by the proteasome.
CC       {ECO:0000250|UniProtKB:Q7Z6J8}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.26;
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: Interacts with UBE2C/UbcH10 (E2 ubiquitin-conjugating enzyme).
CC       {ECO:0000250|UniProtKB:Q7Z6J8}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- DOMAIN: The C-terminal half (AA 188-389) is able to bind cyclin-B and
CC       shows a self-ubiquitination activity (mono-, poly, or multi-
CC       ubiquitination) in a HECT-like sequence dependent manner.
CC       {ECO:0000250|UniProtKB:Q7Z6J8}.
CC   -!- PTM: Ubiquitinated by UBCH10 (E2 ubiquitin-conjugating enzyme).
CC       {ECO:0000250|UniProtKB:Q7Z6J8}.
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DR   EMBL; CR857190; CAH89489.1; -; mRNA.
DR   RefSeq; NP_001124642.1; NM_001131170.1.
DR   AlphaFoldDB; Q5RFG8; -.
DR   STRING; 9601.ENSPPYP00000018809; -.
DR   GeneID; 100171483; -.
DR   KEGG; pon:100171483; -.
DR   CTD; 90025; -.
DR   eggNOG; KOG4784; Eukaryota.
DR   InParanoid; Q5RFG8; -.
DR   OrthoDB; 1587082at2759; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000001595; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR019193; UBQ-conj_enz_E2-bd_prot.
DR   PANTHER; PTHR31531; PTHR31531; 1.
DR   Pfam; PF09814; HECT_2; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Cytoplasm; Reference proteome; Transferase; Ubl conjugation;
KW   Ubl conjugation pathway.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:Q7Z6J8"
FT   CHAIN           2..389
FT                   /note="E3 ubiquitin-protein ligase E3D"
FT                   /id="PRO_0000311192"
FT   REGION          235..257
FT                   /note="Interaction with UBE2C"
FT                   /evidence="ECO:0000250"
FT   REGION          353..389
FT                   /note="HECT-like"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0000250|UniProtKB:Q7Z6J8"
SQ   SEQUENCE   389 AA;  43595 MW;  13DC5C42D79DDEE5 CRC64;
     MAATTAETRV FLEVRGQLQS ALLILGEPKE GGMPMNISIM PSSLQMKTPE GCTEIQLPAE
     VRLVPSSCRG LQFVAGDGLH LRLQAHAELG TKLISMFNQS LQAQECCTFY CQSCGEVIIN
     DRKLLRVLPL PSENWGALVG EWCCHPDPFA NKPLHPQEND CFIGDSFFLV NLRTSLWQQR
     PELSPVEMCC VSSDNHCKLE PKANTKVICK RCKVMLGETV SSETTKFYMT EIIIQSSERS
     FPIIPRPRFV QSVIAQCLVQ LSSARSTFRF MIQGQDDKVY ILLWLLNSDS LVIESLRNSK
     YIKKFPLLED TLKADSSSAW SAVKVLYQPC IKSRNEKLIS SWESDISVHS LTLPSATCLE
     LLLILSKSNA NLPSSLRHMN SFQVAFLKI