UBFL1_MOUSE
ID UBFL1_MOUSE Reviewed; 394 AA.
AC Q3USZ2; Q3TQX0;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Upstream-binding factor 1-like protein 1 {ECO:0000305};
DE AltName: Full=HMG-box preimplantation embryo-specific protein {ECO:0000303|PubMed:19915186};
DE Short=HMGPI {ECO:0000303|PubMed:19915186};
GN Name=Ubtfl1 {ECO:0000312|MGI:MGI:3588290};
GN Synonyms=Hmgpi {ECO:0000303|PubMed:19915186};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Egg;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=19915186; DOI=10.1093/hmg/ddp512;
RA Yamada M., Hamatani T., Akutsu H., Chikazawa N., Kuji N., Yoshimura Y.,
RA Umezawa A.;
RT "Involvement of a novel preimplantation-specific gene encoding the high
RT mobility group box protein Hmgpi in early embryonic development.";
RL Hum. Mol. Genet. 19:480-493(2010).
CC -!- FUNCTION: Essential for proliferation of the inner cell mass and
CC trophectodermal cells in peri-implantation development.
CC {ECO:0000269|PubMed:19915186}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19915186}. Nucleus
CC {ECO:0000255|PROSITE-ProRule:PRU00267, ECO:0000269|PubMed:19915186}.
CC Note=Mainly cytoplasmic from the 4-cell stage to the morula stage.
CC Becomes nuclear at the blastocyst stage. In ES cells, found in both the
CC nucleus and the cytoplasm. {ECO:0000269|PubMed:19915186}.
CC -!- DEVELOPMENTAL STAGE: Transcriptionally activated at the 2-cell stage,
CC peaks at the 4-cell stage and then gradually decreased until the
CC blastocyst stage. The protein is detected from the 4-cell stage until
CC the blastocyst stage. In blastocysts, expressed both by inner cell mass
CC and trophectodermal cells (at protein level). Not detected in post-
CC implentation, neither in fetal, nor adult tissues.
CC {ECO:0000269|PubMed:19915186}.
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DR EMBL; AK139937; BAE24188.1; -; mRNA.
DR EMBL; AK163257; BAE37262.1; -; mRNA.
DR EMBL; CH466522; EDL25044.1; -; Genomic_DNA.
DR EMBL; CH466522; EDL25045.1; -; Genomic_DNA.
DR EMBL; BC139141; AAI39142.1; -; mRNA.
DR EMBL; BC145822; AAI45823.1; -; mRNA.
DR CCDS; CCDS40540.1; -.
DR RefSeq; NP_001028965.1; NM_001033793.3.
DR RefSeq; XP_011240880.1; XM_011242578.1.
DR AlphaFoldDB; Q3USZ2; -.
DR SMR; Q3USZ2; -.
DR STRING; 10090.ENSMUSP00000129611; -.
DR iPTMnet; Q3USZ2; -.
DR PhosphoSitePlus; Q3USZ2; -.
DR PaxDb; Q3USZ2; -.
DR PRIDE; Q3USZ2; -.
DR Antibodypedia; 74795; 22 antibodies from 11 providers.
DR Ensembl; ENSMUST00000098973; ENSMUSP00000096572; ENSMUSG00000074502.
DR Ensembl; ENSMUST00000164441; ENSMUSP00000129611; ENSMUSG00000074502.
DR Ensembl; ENSMUST00000169398; ENSMUSP00000126540; ENSMUSG00000074502.
DR GeneID; 546118; -.
DR KEGG; mmu:546118; -.
DR UCSC; uc009ogq.1; mouse.
DR CTD; 642623; -.
DR MGI; MGI:3588290; Ubtfl1.
DR VEuPathDB; HostDB:ENSMUSG00000074502; -.
DR eggNOG; KOG0381; Eukaryota.
DR GeneTree; ENSGT00940000163858; -.
DR HOGENOM; CLU_021068_0_0_1; -.
DR InParanoid; Q3USZ2; -.
DR OMA; TAYIRFF; -.
DR OrthoDB; 1237679at2759; -.
DR PhylomeDB; Q3USZ2; -.
DR TreeFam; TF328989; -.
DR BioGRID-ORCS; 546118; 0 hits in 70 CRISPR screens.
DR PRO; PR:Q3USZ2; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q3USZ2; protein.
DR Bgee; ENSMUSG00000074502; Expressed in cleaving embryo and 2 other tissues.
DR Genevisible; Q3USZ2; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0001164; F:RNA polymerase I core promoter sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0001181; F:RNA polymerase I general transcription initiation factor activity; IBA:GO_Central.
DR GO; GO:0001832; P:blastocyst growth; IMP:MGI.
DR GO; GO:0007566; P:embryo implantation; IMP:MGI.
DR GO; GO:0045943; P:positive regulation of transcription by RNA polymerase I; IBA:GO_Central.
DR GO; GO:0010468; P:regulation of gene expression; IMP:MGI.
DR GO; GO:0006360; P:transcription by RNA polymerase I; IBA:GO_Central.
DR Gene3D; 1.10.30.10; -; 2.
DR InterPro; IPR009071; HMG_box_dom.
DR InterPro; IPR036910; HMG_box_dom_sf.
DR Pfam; PF00505; HMG_box; 1.
DR SMART; SM00398; HMG; 2.
DR SUPFAM; SSF47095; SSF47095; 2.
DR PROSITE; PS50118; HMG_BOX_2; 2.
PE 1: Evidence at protein level;
KW Cytoplasm; Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Repeat.
FT CHAIN 1..394
FT /note="Upstream-binding factor 1-like protein 1"
FT /id="PRO_0000386641"
FT DNA_BIND 101..169
FT /note="HMG box 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT DNA_BIND 225..291
FT /note="HMG box 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT REGION 167..197
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 305..394
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 315..337
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 352..366
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 373..394
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 344
FT /note="V -> L (in Ref. 1; BAE37262)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 394 AA; 46131 MW; 0F6823F021B5B5B5 CRC64;
MTSLDNQGLW SEKDILKLLE CMEHNIPSDD SREFKKSQAD LNWSKVAFGL FSGEMCKQKW
MEISYNLRKF RTLTELVQEA KFSFTKKTHK NKILTEHPDR PKRPLTAYLR FYKEQRAKYC
QMYPKYSNAQ LTKILAEKYR QLPAEIKQRY IMDFKKEKED FQKKMRQFKK RHPVSGHPKK
SVVPQSHPTK VPTKSQGDIK NVKSLVKTES PRTVSSDMKF QGEPRKPPMN AYHKFHQESW
SSPELRHLSF RKRWVEISRR WHQVPENEKE HYSNQVKRLQ KQYRVKLDLW LKRLSPEEYA
AYKEAKATCG KRKNMSMSGG RSSKFGRTEQ SSSEKGLQIK PGEVEELLDP GTDSSGTIQG
HHDGAQSSRQ DFTDDSEEDD SSTSSDSSST DEDD
