ID   UBGAT_SCUBA             Reviewed;         441 AA.
AC   Q76MR7;
DT   27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Baicalein 7-O-glucuronosyltransferase;
DE            EC=2.4.1.253;
DE   AltName: Full=UDP-glucuronate:baicalein 7-O-glucuronosyltransferase;
GN   Name=UBGAT-I;
OS   Scutellaria baicalensis (Baical skullcap).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Lamiales; Lamiaceae; Scutellarioideae; Scutellaria.
OX   NCBI_TaxID=65409;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Nagashima S., Hirotani M., Yoshikawa T.;
RT   "Cloning and Expression of cDNA encoding UDP-glucuronate:baicalein 7-O-
RT   glucuronosyltransferase from suspension culture of Scutellaria baicalensis
RT   Georgi.";
RL   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ACTIVITY
RP   REGULATION, AND SUBUNIT.
RX   PubMed=10724177; DOI=10.1016/s0031-9422(99)00593-2;
RA   Nagashima S., Hirotani M., Yoshikawa T.;
RT   "Purification and characterization of UDP-glucuronate: baicalein 7-O-
RT   glucuronosyltransferase from Scutellaria baicalensis Georgi. cell
RT   suspension cultures.";
RL   Phytochemistry 53:533-538(2000).
CC   -!- FUNCTION: Involved in the production of glucuronosylated baicalein, a
CC       flavonoid that shows antiallergic, anti-HIV and antitumor activities.
CC       Can use baicalein, scutellarein and wogonin as substrates, but not
CC       chrysin, apigenin, luteolin, quercetin, formononetin and daidzein.
CC       Highly specific for UDP-glucuronate (UDP-GlcUA) and no activity with
CC       UDP-glucose or UDP-galacturonic acid. {ECO:0000269|PubMed:10724177}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=baicalein + UDP-alpha-D-glucuronate = baicalin + UDP;
CC         Xref=Rhea:RHEA:28314, ChEBI:CHEBI:58052, ChEBI:CHEBI:58223,
CC         ChEBI:CHEBI:61283, ChEBI:CHEBI:78324; EC=2.4.1.253;
CC         Evidence={ECO:0000269|PubMed:10724177};
CC   -!- ACTIVITY REGULATION: Inhibited by copper, zinc and iron, p-
CC       Chloromercuri-benzoic acid (PCMBA) and 4,4'-diisothiocyanostilbene-
CC       2,2'-disulfonic acid (DIDS), but not by N-ethylmaleimide (NEM),
CC       dithioerythritol (DTE), calcium or magnesium.
CC       {ECO:0000269|PubMed:10724177}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=52.4 uM for baicalein {ECO:0000269|PubMed:10724177};
CC         KM=109 uM for scutellarein {ECO:0000269|PubMed:10724177};
CC         KM=106.5 uM for wogonin {ECO:0000269|PubMed:10724177};
CC         KM=72.9 uM for UDP-glucuronic acid {ECO:0000269|PubMed:10724177};
CC         Vmax=257 nmol/sec/mg enzyme with baicalein as substrate
CC         {ECO:0000269|PubMed:10724177};
CC         Vmax=290 nmol/sec/mg enzyme with scutellarein as substrate
CC         {ECO:0000269|PubMed:10724177};
CC         Vmax=182 nmol/sec/mg enzyme with wogonin as substrate
CC         {ECO:0000269|PubMed:10724177};
CC       pH dependence:
CC         Optimum pH is 7.5. {ECO:0000269|PubMed:10724177};
CC       Temperature dependence:
CC         Optimum temperature is 30-40 degrees Celsius.
CC         {ECO:0000269|PubMed:10724177};
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:10724177}.
CC   -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AB042277; BAC98300.1; -; mRNA.
DR   AlphaFoldDB; Q76MR7; -.
DR   SMR; Q76MR7; -.
DR   CAZy; GT1; Glycosyltransferase Family 1.
DR   KEGG; ag:BAC98300; -.
DR   BRENDA; 2.4.1.253; 5639.
DR   SABIO-RK; Q76MR7; -.
DR   GO; GO:0102159; F:baicalein 7-O-glucuronosyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0102467; F:scutellarein 7-O-glucuronosyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR   GO; GO:0102468; F:wogonin 7-O-glucuronosyltransferase activity; IEA:UniProtKB-EC.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   Pfam; PF00201; UDPGT; 1.
PE   1: Evidence at protein level;
KW   Glycosyltransferase; Transferase.
FT   CHAIN           1..441
FT                   /note="Baicalein 7-O-glucuronosyltransferase"
FT                   /id="PRO_0000412108"
SQ   SEQUENCE   441 AA;  48654 MW;  C25DB7A0AC292B0B CRC64;
     MAVLAKFISK NHPSVPIIII SNAPESAAAS VAAIPSISYH RLPLPEIPPD MTTDRVELFF
     ELPRLSNPNL LTALQQISQK TRIRAVILDF FCNAAFEVPT SLNIPTYYYF SAGTPTAILT
     LYFETIDETI PVDLQDLNDY VDIPGLPPIH CLDIPVALSP RKSLVYKSSV DISKNLRRSA
     GILVNGFDAL EFRAIGSHSQ RPMHFKGPTP PVYFIGPLVG DVDTKAGSEE HECLRWLDTQ
     PSKSVVFLCF GRRGVFSAKQ LKETAAALEN SGHRFLWSVR NPPELKKATG SDEPDLDELL
     PEGFLERTKD RGFVIKSWAP QKEVLAHDSV GGFVTHCGRS SVSEGVWFGV PMIGWPVDAE
     LRLNRAVMVD DLQVALPLEE EAGGFVTAAE LEKRVRELME TKAGKAVRQR VTELKLSARA
     AVAENGSSLN DLKKFLHATR D