C81D1_ARATH
ID C81D1_ARATH Reviewed; 502 AA.
AC Q9FG65; O65789;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Cytochrome P450 81D1;
DE EC=1.14.-.-;
GN Name=CYP81D1; Synonyms=CYP91A1; OrderedLocusNames=At5g36220;
GN ORFNames=T30G6.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 3-502.
RC STRAIN=cv. Columbia;
RX PubMed=9620263; DOI=10.1023/a:1005921406884;
RA Mizutani M., Ward E., Ohta D.;
RT "Cytochrome P450 superfamily in Arabidopsis thaliana: isolation of cDNAs,
RT differential expression, and RFLP mapping of multiple cytochromes P450.";
RL Plant Mol. Biol. 37:39-52(1998).
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; AB026661; BAB09361.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94058.1; -; Genomic_DNA.
DR EMBL; AY093766; AAM10388.1; -; mRNA.
DR EMBL; BT001045; AAN46799.1; -; mRNA.
DR EMBL; D78606; BAA28538.1; -; mRNA.
DR PIR; T52174; T52174.
DR RefSeq; NP_568533.2; NM_123013.4.
DR AlphaFoldDB; Q9FG65; -.
DR SMR; Q9FG65; -.
DR STRING; 3702.AT5G36220.1; -.
DR PaxDb; Q9FG65; -.
DR PRIDE; Q9FG65; -.
DR ProteomicsDB; 239117; -.
DR EnsemblPlants; AT5G36220.1; AT5G36220.1; AT5G36220.
DR GeneID; 833619; -.
DR Gramene; AT5G36220.1; AT5G36220.1; AT5G36220.
DR KEGG; ath:AT5G36220; -.
DR Araport; AT5G36220; -.
DR TAIR; locus:2183597; AT5G36220.
DR eggNOG; KOG0156; Eukaryota.
DR HOGENOM; CLU_001570_4_0_1; -.
DR InParanoid; Q9FG65; -.
DR OMA; WEYNDEK; -.
DR OrthoDB; 702827at2759; -.
DR PhylomeDB; Q9FG65; -.
DR BioCyc; ARA:AT5G36220-MON; -.
DR PRO; PR:Q9FG65; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FG65; baseline and differential.
DR Genevisible; Q9FG65; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0016709; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen; IBA:GO_Central.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..502
FT /note="Cytochrome P450 81D1"
FT /id="PRO_0000052159"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 440
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
FT CONFLICT 121
FT /note="T -> A (in Ref. 4; BAA28538)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 502 AA; 56724 MW; 1A348A8DD76A41B0 CRC64;
MEETNIRVVL YSIFSLIFLI ISFKFLKPKK QNLPPSPPGW LPIIGHLRLL KPPIHRTLRS
FSETLDHNDG GGVMSLRLGS RLVYVVSSHK VAAEECFGKN DVVLANRPQV IIGKHVGYNN
TNMIAAPYGD HWRNLRRLCT IEIFSTHRLN CFLYVRTDEV RRLISRLSRL AGTKKTVVEL
KPMLMDLTFN NIMRMMTGKR YYGEETTDEE EAKRVRKLVA DVGANTSSGN AVDYVPILRL
FSSYENRVKK LGEETDKFLQ GLIDDKRGQQ ETGTTMIDHL LVLQKSDIEY YTDQIIKGII
LIMVIAGTNT SAVTLEWALS NLLNHPDVIS KARDEIDNRV GLDRLIEEAD LSELPYLKNI
VLETLRLHPA TPLLVPHMAS EDCKIGSYDM PRGTTLLVNA WAIHRDPNTW DDPDSFKPER
FEKEEEAQKL LAFGLGRRAC PGSGLAQRIV GLALGSLIQC FEWERVGNVE VDMKEGVGNT
VPKAIPLKAI CKARPFLHKI IS
