ACBSY_ORYSJ
ID ACBSY_ORYSJ Reviewed; 760 AA.
AC Q2R712; A0A0P0Y1F2; Q53MS8;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2006, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Achilleol B synthase;
DE EC=5.4.99.48;
GN OrderedLocusNames=Os11g0285000, LOC_Os11g18194;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16188032; DOI=10.1186/1741-7007-3-20;
RG The rice chromosomes 11 and 12 sequencing consortia;
RT "The sequence of rice chromosomes 11 and 12, rich in disease resistance
RT genes and recent gene duplications.";
RL BMC Biol. 3:20-20(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=21526825; DOI=10.1021/ol200777d;
RA Ito R., Mori K., Hashimoto I., Nakano C., Sato T., Hoshino T.;
RT "Triterpene cyclases from Oryza sativa L.: cycloartenol, parkeol and
RT achilleol B synthases.";
RL Org. Lett. 13:2678-2681(2011).
CC -!- FUNCTION: Specifically mediates the conversion of oxidosqualene ((3S)-
CC 2,3-epoxy-2,3-dihydrosqualene) to achilleol B. Achilleol B is probably
CC formed by cleavage of the 8-14 and 9-10 bonds of (3S)-2,3-epoxy-2,3-
CC dihydrosqualene as part of the cyclization reaction, after formation of
CC the oleanane skeleton. {ECO:0000269|PubMed:21526825}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-2,3-epoxysqualene = achilleol B; Xref=Rhea:RHEA:31855,
CC ChEBI:CHEBI:15441, ChEBI:CHEBI:63461; EC=5.4.99.48;
CC Evidence={ECO:0000269|PubMed:21526825};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAX96835.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC135562; AAX96835.1; ALT_SEQ; Genomic_DNA.
DR EMBL; DP000010; ABA92749.2; -; Genomic_DNA.
DR EMBL; AP008217; BAF28063.1; -; Genomic_DNA.
DR EMBL; AP014967; BAT13616.1; -; Genomic_DNA.
DR EMBL; AK070534; BAG92014.1; -; mRNA.
DR RefSeq; XP_015615776.1; XM_015760290.1.
DR AlphaFoldDB; Q2R712; -.
DR SMR; Q2R712; -.
DR STRING; 4530.OS11T0285000-01; -.
DR PaxDb; Q2R712; -.
DR PRIDE; Q2R712; -.
DR EnsemblPlants; Os11t0285000-01; Os11t0285000-01; Os11g0285000.
DR GeneID; 4350288; -.
DR Gramene; Os11t0285000-01; Os11t0285000-01; Os11g0285000.
DR KEGG; osa:4350288; -.
DR eggNOG; KOG0497; Eukaryota.
DR HOGENOM; CLU_009074_2_0_1; -.
DR InParanoid; Q2R712; -.
DR OMA; QGRMWIL; -.
DR OrthoDB; 365003at2759; -.
DR BioCyc; MetaCyc:MON-17970; -.
DR Proteomes; UP000000763; Chromosome 11.
DR Proteomes; UP000059680; Chromosome 11.
DR Genevisible; Q2R712; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR GO; GO:0016866; F:intramolecular transferase activity; IEA:InterPro.
DR GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro.
DR CDD; cd02892; SQCY_1; 1.
DR InterPro; IPR032696; SQ_cyclase_C.
DR InterPro; IPR032697; SQ_cyclase_N.
DR InterPro; IPR018333; Squalene_cyclase.
DR InterPro; IPR002365; Terpene_synthase_CS.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR PANTHER; PTHR11764; PTHR11764; 1.
DR Pfam; PF13243; SQHop_cyclase_C; 1.
DR Pfam; PF13249; SQHop_cyclase_N; 1.
DR SUPFAM; SSF48239; SSF48239; 2.
DR TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE 1: Evidence at protein level;
KW Isomerase; Membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..760
FT /note="Achilleol B synthase"
FT /id="PRO_0000418966"
FT TRANSMEM 612..632
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REPEAT 153..194
FT /note="PFTB 1"
FT REPEAT 642..688
FT /note="PFTB 2"
FT ACT_SITE 487
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:P48449"
SQ SEQUENCE 760 AA; 86636 MW; FFBD7B1F53C56897 CRC64;
MWRLKIAAES GGGSGSSPLL HTGNGFLGRA VWEFDPDAGT PEERAEVARL RRDFTRHRFQ
RKESQDLLMR MQYAKLGHLQ PDLSAVIVED NQNVTEETIL SSLRRALNQY STLQAHDGHW
PGDYSGILFI MPLLIFSMHV TGTLDVVLSL EHKREICRYI YNHQNEDGGW GTQVLGQSTM
FGSCLNYATL KLLGEALHNN DALAQGRMWI LSHGSATAAP QWAKIWLSVI GVYDWSGNKA
IIPELWMVPH FLPIHPARFW CFVRMIYMSM AYLYGKKFVG PITPTILEIR EELYNIPYSE
IDWKKARDCC AKEDLRYPCS WIQDIVWTYL NKYVDPMFNV WPFNKLREIS LRNLMKHIYY
EDENTKYIGL CPINKALNMI CCWIEDPNSD AFKRHLPRIY DFLWLAEDGM KAQVYDGCQT
WETAFIVQAI CSTGLVDEFS TTLEKAYGFL KNSQVLHDLP NGKSFYRHRS KGSWTLSTAD
NGWSVPDCTG ETLQALLGLS KISPKLVGDP IKEKSLYDAV DCLLSFSNKD GTFSSYECTR
TASWTEILNP SESFRNIVVD YPHVECTSSA IQGLISFTEL YPGYRGVEIE SCIKNAVMFI
ENKQQNDGSW YGTWGICFTY GAFFAIRGLI AAGRNYENSQ AIRNGCKFLL SKQLSAGGWG
EHYSSSEIEV YVDSGSPHAV NTSLAMLALL YSGQIERDPT PLYRAAKQLI SMQLETGEFP
QQEHVGCFNS SLYFNYPNYR NLYPIWALGE FWHRLVASKD
