C85A1_BRADI
ID C85A1_BRADI Reviewed; 466 AA.
AC I1GQE7;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 13-JUN-2012, sequence version 1.
DT 03-AUG-2022, entry version 52.
DE RecName: Full=Cytochrome P450 85A1 {ECO:0000305};
DE Short=BdCYP85A1 {ECO:0000303|PubMed:32146811};
DE AltName: Full=3-dehydroteasterone synthase {ECO:0000303|PubMed:32146811};
DE EC=1.14.14.- {ECO:0000269|PubMed:32146811};
DE AltName: Full=Castasterone synthase {ECO:0000303|PubMed:32146811};
DE EC=1.14.14.- {ECO:0000269|PubMed:32146811};
DE AltName: Full=Teasterone synthase {ECO:0000303|PubMed:32146811};
DE EC=1.14.14.- {ECO:0000269|PubMed:32146811};
DE AltName: Full=Typhasterol synthase {ECO:0000303|PubMed:32146811};
DE EC=1.14.14.- {ECO:0000269|PubMed:32146811};
GN Name=CYP85A1 {ECO:0000303|PubMed:32146811};
GN OrderedLocusNames=BRADI_1g15030v3 {ECO:0000312|EMBL:KQK14264.1};
OS Brachypodium distachyon (Purple false brome) (Trachynia distachya).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Stipodae; Brachypodieae; Brachypodium.
OX NCBI_TaxID=15368;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Bd21;
RX PubMed=20148030; DOI=10.1038/nature08747;
RG International Brachypodium Initiative;
RT "Genome sequencing and analysis of the model grass Brachypodium
RT distachyon.";
RL Nature 463:763-768(2010).
RN [2]
RP FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RX PubMed=32146811; DOI=10.1021/acs.jafc.9b07963;
RA Roh J., Moon J., Youn J.-H., Seo C., Park Y.J., Kim S.-K.;
RT "Establishment of biosynthetic pathways to generate castasterone as the
RT biologically active brassinosteroid in Brachypodium distachyon.";
RL J. Agric. Food Chem. 68:3912-3923(2020).
CC -!- FUNCTION: Involved in reduction steps of the biosynthesis of plant
CC campesterol-derivative steroids, ending to castasterone (CS) but
CC missing brassinolide (BL) (PubMed:32146811). Catalyzes the C6-oxidation
CC step in brassinosteroids biosynthesis; the conversion of 6-
CC deoxoteasterone (6-deoxoTE) to teasterone (TE), 3-dehydro-6-
CC deoxoteasterone (6-deoxo3DT, 6-deoxo-3-DHT) to 3-dehydroteasterone
CC (3DT, 3-DHT), 6-deoxotyphasterol (6-deoxoTY) to typhasterol (TY) and of
CC 6-deoxocastasterone (6-deoxoCS) to castasterone (CS) (PubMed:32146811).
CC {ECO:0000269|PubMed:32146811}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=6-deoxoteasterone + O2 + reduced [NADPH--hemoprotein
CC reductase] = 6alpha-hydroxyteasterone + H(+) + H2O + oxidized
CC [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:69959, Rhea:RHEA-
CC COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:20716,
CC ChEBI:CHEBI:57618, ChEBI:CHEBI:58210, ChEBI:CHEBI:188499;
CC Evidence={ECO:0000269|PubMed:32146811};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:69960;
CC Evidence={ECO:0000269|PubMed:32146811};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=6alpha-hydroxytyphasterol + O2 + reduced [NADPH--hemoprotein
CC reductase] = H(+) + 2 H2O + oxidized [NADPH--hemoprotein reductase] +
CC teasterone; Xref=Rhea:RHEA:69963, Rhea:RHEA-COMP:11964, Rhea:RHEA-
CC COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:26863, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC ChEBI:CHEBI:188495; Evidence={ECO:0000269|PubMed:32146811};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:69964;
CC Evidence={ECO:0000269|PubMed:32146811};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-dehydro-6-deoxoteasterone + O2 + reduced [NADPH--hemoprotein
CC reductase] = 3-dehydro-6alpha-hydroxyteasterone + H(+) + H2O +
CC oxidized [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:69947,
CC Rhea:RHEA-COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:20710,
CC ChEBI:CHEBI:57618, ChEBI:CHEBI:58210, ChEBI:CHEBI:188496;
CC Evidence={ECO:0000269|PubMed:32146811};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:69948;
CC Evidence={ECO:0000269|PubMed:32146811};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-dehydro-6alpha-hydroxyteasterone + O2 + reduced [NADPH--
CC hemoprotein reductase] = 3-dehydroteasterone + H(+) + 2 H2O +
CC oxidized [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:69951,
CC Rhea:RHEA-COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:20000,
CC ChEBI:CHEBI:57618, ChEBI:CHEBI:58210, ChEBI:CHEBI:188496;
CC Evidence={ECO:0000269|PubMed:32146811};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:69952;
CC Evidence={ECO:0000269|PubMed:32146811};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=6-deoxotyphasterol + O2 + reduced [NADPH--hemoprotein
CC reductase] = 6alpha-hydroxytyphasterol + H(+) + H2O + oxidized
CC [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:69939, Rhea:RHEA-
CC COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:20717,
CC ChEBI:CHEBI:57618, ChEBI:CHEBI:58210, ChEBI:CHEBI:188495;
CC Evidence={ECO:0000269|PubMed:32146811};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:69940;
CC Evidence={ECO:0000269|PubMed:32146811};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=6alpha-hydroxytyphasterol + O2 + reduced [NADPH--hemoprotein
CC reductase] = H(+) + 2 H2O + oxidized [NADPH--hemoprotein reductase] +
CC typhasterol; Xref=Rhea:RHEA:69943, Rhea:RHEA-COMP:11964, Rhea:RHEA-
CC COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:27173, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC ChEBI:CHEBI:188495; Evidence={ECO:0000269|PubMed:32146811};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:69944;
CC Evidence={ECO:0000269|PubMed:32146811};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=6-deoxocastasterone + O2 + reduced [NADPH--hemoprotein
CC reductase] = 6alpha-hydroxycastasterone + H(+) + H2O + oxidized
CC [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:69875, Rhea:RHEA-
CC COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:20712,
CC ChEBI:CHEBI:20760, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210;
CC Evidence={ECO:0000269|PubMed:32146811};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:69876;
CC Evidence={ECO:0000269|PubMed:32146811};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=6alpha-hydroxycastasterone + O2 + reduced [NADPH--hemoprotein
CC reductase] = castasterone + H(+) + 2 H2O + oxidized [NADPH--
CC hemoprotein reductase]; Xref=Rhea:RHEA:69879, Rhea:RHEA-COMP:11964,
CC Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:20760, ChEBI:CHEBI:23051,
CC ChEBI:CHEBI:57618, ChEBI:CHEBI:58210;
CC Evidence={ECO:0000269|PubMed:32146811};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:69880;
CC Evidence={ECO:0000269|PubMed:32146811};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-dehydro-6-deoxoteasterone + 2 O2 + 2 reduced [NADPH--
CC hemoprotein reductase] = 3-dehydroteasterone + 2 H(+) + 3 H2O + 2
CC oxidized [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:70039,
CC Rhea:RHEA-COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:20000,
CC ChEBI:CHEBI:20710, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210;
CC Evidence={ECO:0000269|PubMed:32146811};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:70040;
CC Evidence={ECO:0000269|PubMed:32146811};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=6-deoxocastasterone + 2 O2 + 2 reduced [NADPH--hemoprotein
CC reductase] = castasterone + 2 H(+) + 3 H2O + 2 oxidized [NADPH--
CC hemoprotein reductase]; Xref=Rhea:RHEA:70031, Rhea:RHEA-COMP:11964,
CC Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:20712, ChEBI:CHEBI:23051,
CC ChEBI:CHEBI:57618, ChEBI:CHEBI:58210;
CC Evidence={ECO:0000269|PubMed:32146811};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:70032;
CC Evidence={ECO:0000269|PubMed:32146811};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=6-deoxoteasterone + 2 O2 + 2 reduced [NADPH--hemoprotein
CC reductase] = 2 H(+) + 3 H2O + 2 oxidized [NADPH--hemoprotein
CC reductase] + teasterone; Xref=Rhea:RHEA:70043, Rhea:RHEA-COMP:11964,
CC Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:20716, ChEBI:CHEBI:26863,
CC ChEBI:CHEBI:57618, ChEBI:CHEBI:58210;
CC Evidence={ECO:0000269|PubMed:32146811};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:70044;
CC Evidence={ECO:0000269|PubMed:32146811};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=6-deoxotyphasterol + 2 O2 + 2 reduced [NADPH--hemoprotein
CC reductase] = 2 H(+) + 3 H2O + 2 oxidized [NADPH--hemoprotein
CC reductase] + typhasterol; Xref=Rhea:RHEA:70035, Rhea:RHEA-COMP:11964,
CC Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:20717, ChEBI:CHEBI:27173,
CC ChEBI:CHEBI:57618, ChEBI:CHEBI:58210;
CC Evidence={ECO:0000269|PubMed:32146811};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:70036;
CC Evidence={ECO:0000269|PubMed:32146811};
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413;
CC Evidence={ECO:0000250|UniProtKB:P04798};
CC -!- PATHWAY: Plant hormone biosynthesis; brassinosteroid biosynthesis.
CC {ECO:0000269|PubMed:32146811}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; CM000880; KQK14264.1; -; Genomic_DNA.
DR RefSeq; XP_003562306.1; XM_003562258.3.
DR STRING; 15368.BRADI1G15030.1; -.
DR EnsemblPlants; KQK14264; KQK14264; BRADI_1g15030v3.
DR GeneID; 100837653; -.
DR Gramene; KQK14264; KQK14264; BRADI_1g15030v3.
DR KEGG; bdi:100837653; -.
DR eggNOG; KOG0157; Eukaryota.
DR HOGENOM; CLU_001570_15_5_1; -.
DR InParanoid; I1GQE7; -.
DR OMA; WTATRDR; -.
DR OrthoDB; 574756at2759; -.
DR UniPathway; UPA00381; -.
DR Proteomes; UP000008810; Chromosome 1.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IBA:GO_Central.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0016132; P:brassinosteroid biosynthetic process; IBA:GO_Central.
DR GO; GO:0010268; P:brassinosteroid homeostasis; IBA:GO_Central.
DR GO; GO:0001578; P:microtubule bundle formation; IEA:EnsemblPlants.
DR GO; GO:0009647; P:skotomorphogenesis; IEA:EnsemblPlants.
DR GO; GO:0016125; P:sterol metabolic process; IBA:GO_Central.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 1: Evidence at protein level;
KW Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..466
FT /note="Cytochrome P450 85A1"
FT /id="PRO_0000455311"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 416
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P04798"
SQ SEQUENCE 466 AA; 52782 MW; EE111F147C02AF72 CRC64;
MVLAVLIGVL VGIVLVSSLL LRWNEVRYSR KQGLPPGTMG WPLFGETTEF LKQGPSFMKA
RRLRYGSLFR THILGCPTVV CMDPELNRQM LQQGEGRGFV PGYPQSMLDI LGRNNIAAVH
GPLHRAMRGS MLALVRPASI RSSLLPKIDA FMRSHLHGWA GDLVDIQDKT KEMALLSALR
QIAGITAGPL SDALKTELYT LVLGTISLPI NLPGTSYYQG FQARKKLVSM LEKMIAERRS
SGLVHNDMLD ALLSGNDGTR ERLSDEQIID LIITLIYSGY ETMSTTSMMA VKYLSDHPKA
LEELRKEHFD IRKGKSPEEA IDYNDFKSMT FTRAVIFETL RLATVVNGLL RKTTQDVEMN
GYVIPKGWRI YVYTREINYD PCLYPDPMTF NPWRWLEKNM ESHPHFMLFG GGGRMCPGKE
VGTAEIATFL HYFVTRYRWE EEGTNTILKF PRVEAPNGLH IRVQNY
