ID   C88A1_MAIZE             Reviewed;         519 AA.
AC   Q43246;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=Cytochrome P450 88A1;
DE            EC=1.14.-.-;
DE   AltName: Full=Dwarf3 protein;
GN   Name=CYP88A1; Synonyms=D3;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. B73;
RX   PubMed=7549486; DOI=10.2307/3870104;
RA   Winkler R.G., Helentjaris T.;
RT   "The maize Dwarf3 gene encodes a cytochrome P450-mediated early step in
RT   Gibberellin biosynthesis.";
RL   Plant Cell 7:1307-1317(1995).
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- PATHWAY: Plant hormone biosynthesis; gibberellin biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, developing leaves, the
CC       vegetative meristem, and suspension culture cells.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; U32579; AAC49067.1; -; mRNA.
DR   PIR; T02263; T02263.
DR   RefSeq; NP_001105586.1; NM_001112116.1.
DR   AlphaFoldDB; Q43246; -.
DR   SMR; Q43246; -.
DR   STRING; 4577.GRMZM2G093195_P01; -.
DR   PaxDb; Q43246; -.
DR   PRIDE; Q43246; -.
DR   EnsemblPlants; Zm00001eb379120_T002; Zm00001eb379120_P002; Zm00001eb379120.
DR   GeneID; 542580; -.
DR   Gramene; Zm00001eb379120_T002; Zm00001eb379120_P002; Zm00001eb379120.
DR   KEGG; zma:542580; -.
DR   eggNOG; KOG0157; Eukaryota.
DR   HOGENOM; CLU_001570_15_5_1; -.
DR   OrthoDB; 825914at2759; -.
DR   UniPathway; UPA00390; -.
DR   Proteomes; UP000007305; Chromosome 9.
DR   ExpressionAtlas; Q43246; baseline and differential.
DR   Genevisible; Q43246; ZM.
DR   GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0051777; F:ent-kaurenoate oxidase activity; IBA:GO_Central.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IBA:GO_Central.
DR   GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR   GO; GO:0016132; P:brassinosteroid biosynthetic process; IBA:GO_Central.
DR   GO; GO:0010268; P:brassinosteroid homeostasis; IBA:GO_Central.
DR   GO; GO:0009686; P:gibberellin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0016125; P:sterol metabolic process; IBA:GO_Central.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR002397; Cyt_P450_B.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00359; BP450.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..519
FT                   /note="Cytochrome P450 88A1"
FT                   /id="PRO_0000052177"
FT   TRANSMEM        1..21
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         466
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   519 AA;  57906 MW;  0F8977A024316D95 CRC64;
     MLGVGMAAAV LLGAVALLLA DAAARRAHWW YREAAEAVLV GAVALVVVDA AARRAHGWYR
     EAALGAARRA RLPPGEMGWP LVGGMWAFLR AFKSGKPDAF IASFVRRFGR TGVYRSFMFS
     SPTVLVTTAE GCKQVLMDDD AFVTGWPKAT VALVGPRSFV AMPYDEHRRI RKLTAAPING
     FDALTGYLPF IDRTVTSSLR AWADHGGSVE FLTELRRMTF KIIVQIFLGG ADQATTRALE
     RSYTELNYGM RAMAINLPGF AYRGALRARR RLVAVLQGVL DERRAARAKG VSGGGVDMMD
     RLIEAQDERG RHLDDDEIID VLVMYLNAGH ESSGHITMWA TVFLQENPDM FARAKAEQEA
     IMRSIPSSQR GLTLRDFRKM EYLSQVIDET LRLVNISFVS FRQATRDVFV NGYLIPKGWK
     VQLWYRSVHM DPQVYPDPTK FDPSRWEGHS PRAGTFLAFG LGARLCPGND LAKLEISVFL
     HHFLLGYKLA RTNPRCRVRY LPHPRPVDNC LAKITRVGS