C90A1_BRADI
ID C90A1_BRADI Reviewed; 498 AA.
AC I1IUJ6;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 13-JUN-2012, sequence version 1.
DT 03-AUG-2022, entry version 51.
DE RecName: Full=Cytochrome P450 90A1 {ECO:0000305};
DE Short=BdCYP90A1 {ECO:0000303|PubMed:32146811};
DE AltName: Full=(22S,24R)-22-hydroxyergost-4-en-3-one synthase {ECO:0000303|PubMed:32146811};
DE EC=1.14.19.- {ECO:0000269|PubMed:32146811};
DE AltName: Full=3-dehydro-6-deoxoteasterone synthase {ECO:0000303|PubMed:32146811};
DE EC=1.14.19.- {ECO:0000269|PubMed:32146811};
GN Name=CYP90A1 {ECO:0000312|EnsemblPlants:KQJ92356};
GN OrderedLocusNames=BRADI_4g43110v3 {ECO:0000312|EMBL:KQJ92356.1};
OS Brachypodium distachyon (Purple false brome) (Trachynia distachya).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Stipodae; Brachypodieae; Brachypodium.
OX NCBI_TaxID=15368;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Bd21;
RX PubMed=20148030; DOI=10.1038/nature08747;
RG International Brachypodium Initiative;
RT "Genome sequencing and analysis of the model grass Brachypodium
RT distachyon.";
RL Nature 463:763-768(2010).
RN [2]
RP FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RX PubMed=32146811; DOI=10.1021/acs.jafc.9b07963;
RA Roh J., Moon J., Youn J.-H., Seo C., Park Y.J., Kim S.-K.;
RT "Establishment of biosynthetic pathways to generate castasterone as the
RT biologically active brassinosteroid in Brachypodium distachyon.";
RL J. Agric. Food Chem. 68:3912-3923(2020).
CC -!- FUNCTION: Involved in reduction steps of the biosynthesis of plant
CC campesterol-derivative steroids, ending to castasterone (CS) but
CC missing brassinolide (BL) (PubMed:32146811). Catalyzes the conversion
CC of (22S)-22-hydroxycampesterol (22-hydroxyCR, 22-OHCR) to (22S,24R)-22-
CC hydroxyergost-4-en-3-one (22-hydroxy-campesta-4-en-3-one, 22-OH-4-en-3-
CC one) and of 6-deoxoteasterone (6-deoxoTE) to 3-dehydro-6-
CC deoxoteasterone (6-deoxo3DT, 6-deoxo3DHT) (PubMed:32146811).
CC {ECO:0000269|PubMed:32146811}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(22S)-22-hydroxycampesterol + O2 + reduced [NADPH--hemoprotein
CC reductase] = (22S)-22-hydroxycampest-4-en-3-one + H(+) + 2 H2O +
CC oxidized [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:69971,
CC Rhea:RHEA-COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:57618,
CC ChEBI:CHEBI:58210, ChEBI:CHEBI:72330, ChEBI:CHEBI:72331;
CC Evidence={ECO:0000269|PubMed:32146811};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:69972;
CC Evidence={ECO:0000269|PubMed:32146811};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=6-deoxoteasterone + O2 + reduced [NADPH--hemoprotein
CC reductase] = 3-dehydro-6-deoxoteasterone + H(+) + 2 H2O + oxidized
CC [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:69983, Rhea:RHEA-
CC COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:20710,
CC ChEBI:CHEBI:20716, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210;
CC Evidence={ECO:0000269|PubMed:32146811};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:69984;
CC Evidence={ECO:0000269|PubMed:32146811};
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413;
CC Evidence={ECO:0000250|UniProtKB:P04798};
CC -!- PATHWAY: Plant hormone biosynthesis; brassinosteroid biosynthesis.
CC {ECO:0000269|PubMed:32146811}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; CM000883; KQJ92356.1; -; Genomic_DNA.
DR RefSeq; XP_010238746.1; XM_010240444.2.
DR STRING; 15368.BRADI4G43110.1; -.
DR EnsemblPlants; KQJ92356; KQJ92356; BRADI_4g43110v3.
DR GeneID; 100831598; -.
DR Gramene; KQJ92356; KQJ92356; BRADI_4g43110v3.
DR KEGG; bdi:100831598; -.
DR eggNOG; KOG0157; Eukaryota.
DR HOGENOM; CLU_001570_15_5_1; -.
DR InParanoid; I1IUJ6; -.
DR OMA; KLWEVYM; -.
DR OrthoDB; 1247045at2759; -.
DR UniPathway; UPA00381; -.
DR Proteomes; UP000008810; Chromosome 4.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IBA:GO_Central.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0048657; P:anther wall tapetum cell differentiation; IEA:EnsemblPlants.
DR GO; GO:0016132; P:brassinosteroid biosynthetic process; IBA:GO_Central.
DR GO; GO:0010268; P:brassinosteroid homeostasis; IBA:GO_Central.
DR GO; GO:0010584; P:pollen exine formation; IEA:EnsemblPlants.
DR GO; GO:0009911; P:positive regulation of flower development; IEA:EnsemblPlants.
DR GO; GO:0010224; P:response to UV-B; IEA:EnsemblPlants.
DR GO; GO:0016125; P:sterol metabolic process; IBA:GO_Central.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 1: Evidence at protein level;
KW Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..498
FT /note="Cytochrome P450 90A1"
FT /id="PRO_0000455306"
FT TRANSMEM 5..25
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 257..277
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 447
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P04798"
SQ SEQUENCE 498 AA; 55778 MW; EE47942BE1266032 CRC64;
MENAGALQLL SAAAAAVAVV VVVALRWLLA RRAAGGWKQR PRLPPGSTGL PLIGETLRLI
SAYKTPDPEP FIDERVARHG GVFTTHVFGE RTVFSADPAF NRLLLAAEGR AVDCSYPSSI
TTLLGARSLL LTRGAAHKRL HSLTLTRLGR PASPPLLAHI DRLVLDTMRH WEPAATVRLL
DEAKKITFNL TVKQLVSIDP GPWTESLRRE YVKLIDGFFS IPFPFASLLP FTTYGQALKS
RKKVAGALRE VIRKRMEERG EESGATEEAE SKREKKDMVE ELLQAEGGSF SEEEMVDFCL
SLLVAGYETT SVLMTVAVKF LTETPAALAQ LKEEHENMTN MKGENQPLEW SDYKSMTFTQ
CVINETLRVA NIISGVFRRA NTDIHFKGYT IPKGCKIFAS FRAVHLNNDH YENARTFDPW
RWQSNHKLQN EVGANLFTPF GGGPRLCPGY ELARVVISVF LHHLVMRFSW EAAEEDRLVF
FPTTRTLKGY PINLRQRS
