C90A4_ORYSJ
ID C90A4_ORYSJ Reviewed; 501 AA.
AC Q5CCK1;
DT 15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT 12-APR-2005, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Cytochrome P450 90A4 {ECO:0000305};
DE Short=OsCYP90A4 {ECO:0000305};
DE EC=1.14.99.- {ECO:0000305|Ref.7};
DE AltName: Full=Protein CONSTITUTIVE PHOTOMORPHOGENESIS AND DWARFISM 2 {ECO:0000303|Ref.7};
DE Short=OsCPD2 {ECO:0000303|Ref.7};
DE AltName: Full=Steroid 23-alpha-hydroxylase {ECO:0000305};
GN Name=CYP90A4 {ECO:0000303|Ref.7}; Synonyms=CPD2 {ECO:0000303|Ref.7};
GN OrderedLocusNames=Os12g0139300 {ECO:0000312|EMBL:BAF29136.1},
GN LOC_Os12g04480 {ECO:0000312|EMBL:ABA95785.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16369540; DOI=10.1038/nbt1173;
RA Sakamoto T., Morinaka Y., Ohnishi T., Sunohara H., Fujioka S.,
RA Ueguchi-Tanaka M., Mizutani M., Sakata K., Takatsuto S., Yoshida S.,
RA Tanaka H., Kitano H., Matsuoka M.;
RT "Erect leaves caused by brassinosteroid deficiency increase biomass
RT production and grain yield in rice.";
RL Nat. Biotechnol. 24:105-109(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16188032; DOI=10.1186/1741-7007-3-20;
RG The rice chromosomes 11 and 12 sequencing consortia;
RT "The sequence of rice chromosomes 11 and 12, rich in disease resistance
RT genes and recent gene duplications.";
RL BMC Biol. 3:20-20(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX DOI=10.1007/s00344-006-0041-6;
RA Sakamoto T., Matsuoka M.;
RT "Characterization of CONSTITUTIVE PHOTOMORPHOGENESIS AND DWARFISM homologs
RT in rice (Oryza sativa L.).";
RL J. Plant Growth Regul. 25:245-251(2006).
CC -!- FUNCTION: Catalyzes the C23-alpha-hydroxylation step in brassinosteroid
CC biosynthesis (Probable). Converts 6-deoxocathasterone to 6-
CC deoxoteasterone in the late C6-oxidation pathway and cathasterone to
CC teasterone (TE) in the early C6-oxidation pathway of brassinolide (BL)
CC biosynthesis (Probable). {ECO:0000305|Ref.7}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413;
CC Evidence={ECO:0000250|UniProtKB:P04798};
CC -!- PATHWAY: Plant hormone biosynthesis; brassinosteroid biosynthesis.
CC {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Highly expressed in shoot apex and inflorenscence.
CC Expressed in roots, stems, leaf blades and leaf sheaths.
CC {ECO:0000269|Ref.7}.
CC -!- INDUCTION: Down-regulated by brassinolide (BL). {ECO:0000269|Ref.7}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; AB206581; BAD90974.1; -; mRNA.
DR EMBL; DP000011; ABA95785.1; -; Genomic_DNA.
DR EMBL; AP008218; BAF29136.1; -; Genomic_DNA.
DR EMBL; AP014968; BAT15826.1; -; Genomic_DNA.
DR EMBL; AK060257; BAG87383.1; -; mRNA.
DR RefSeq; XP_015619622.1; XM_015764136.1.
DR AlphaFoldDB; Q5CCK1; -.
DR SMR; Q5CCK1; -.
DR STRING; 4530.OS12T0139300-01; -.
DR PaxDb; Q5CCK1; -.
DR PRIDE; Q5CCK1; -.
DR EnsemblPlants; Os12t0139300-01; Os12t0139300-01; Os12g0139300.
DR GeneID; 4351461; -.
DR Gramene; Os12t0139300-01; Os12t0139300-01; Os12g0139300.
DR KEGG; osa:4351461; -.
DR eggNOG; KOG0157; Eukaryota.
DR HOGENOM; CLU_001570_15_5_1; -.
DR InParanoid; Q5CCK1; -.
DR OMA; IEPCEWT; -.
DR OrthoDB; 1247045at2759; -.
DR PlantReactome; R-OSA-1119456; Brassinosteroid biosynthesis II.
DR UniPathway; UPA00381; -.
DR Proteomes; UP000000763; Chromosome 12.
DR Proteomes; UP000059680; Chromosome 12.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0080132; F:fatty acid alpha-hydroxylase activity; IDA:UniProtKB.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IBA:GO_Central.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0016132; P:brassinosteroid biosynthetic process; IDA:UniProtKB.
DR GO; GO:0010268; P:brassinosteroid homeostasis; IBA:GO_Central.
DR GO; GO:0016125; P:sterol metabolic process; IBA:GO_Central.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Brassinosteroid biosynthesis; Cell membrane; Heme; Iron;
KW Lipid biosynthesis; Lipid metabolism; Membrane; Metal-binding;
KW Monooxygenase; Oxidoreductase; Reference proteome; Steroid biosynthesis;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..501
FT /note="Cytochrome P450 90A4"
FT /id="PRO_0000439013"
FT TRANSMEM 2..22
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 446
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P04798"
SQ SEQUENCE 501 AA; 55720 MW; 5A706ECB5F14CF88 CRC64;
MAAAALLLLA AAAAAVVVAM ALRWLLLLGG PAAGRLGKRA RMPPGSTGLP LIGETLRLIS
AYKTPNPEPF IDERVARHGG VFTTHVFGER TVFSADPAFN RLLLAAEGRA VHSSYPSSIA
TLLGARSLLL TRGAAHKRLH SLTLTRLGRP ASPPLLAHID RLVLATMRQW EPAATVRLMD
EAKKITFNLT VKQLVSIEPG PWTESLRREY VKLIDGFFSI PFPLAYFLPF TTYGQALKAR
KKVAGALREV IKKRMEEKAE NGGSIGDDEG KKEKKDMVEE LLQAEGGSFS EEEMVDFCLS
LLVAGYETTS VLMTLAVKFL TETPAALAEL KEEHANIRDM KGKNQPLEWS DYKSMPFTQC
VINETLRVGN IISGVFRRAN TDIHYKDYTI PKGCKIFASF RAVHLNNEHY ENARTFNPWR
WQINNKLQNA VGANIFTPFG GGPRLCPGYE LARVVVSIFL HHLVTRFSWE ETEEDRLVFF
PTTRTLKGYP INLRLLSESI C
