C90B2_ORYSI
ID C90B2_ORYSI Reviewed; 506 AA.
AC B8AJL3;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=Cytochrome P450 90B2 {ECO:0000250|UniProtKB:Q5CCK3};
DE Short=OsCYP90B2 {ECO:0000250|UniProtKB:Q5CCK3};
DE EC=1.14.13.- {ECO:0000250|UniProtKB:Q5CCK3};
DE AltName: Full=(22S)-22-hydroxycampesterol synthase {ECO:0000250|UniProtKB:Q5CCK3};
DE EC=1.14.14.- {ECO:0000250|UniProtKB:Q5CCK3};
DE AltName: Full=6-deoxocathasterone synthase {ECO:0000250|UniProtKB:Q5CCK3};
DE EC=1.14.14.- {ECO:0000250|UniProtKB:Q5CCK3};
GN Name=CYP90B2 {ECO:0000250|UniProtKB:Q5CCK3};
GN ORFNames=OsI_10612 {ECO:0000312|EMBL:EEC74804.1};
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- FUNCTION: Catalyzes the C22-alpha-hydroxylation step in brassinosteroid
CC biosynthesis, which is the rate-limiting step in this biosynthetic
CC pathway (By similarity). Catalyzes the conversion of campesterol (CR)
CC to (22S)-22-hydroxycampesterol (22-OHCR, 22-hydroxyCR) and of
CC campestanol (CN) to 6-deoxocathasterone (6-deoxoCT) (By similarity).
CC {ECO:0000250|UniProtKB:Q5CCK3}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=campesterol + O2 + reduced [NADPH--hemoprotein reductase] =
CC (22S)-22-hydroxycampesterol + H(+) + H2O + oxidized [NADPH--
CC hemoprotein reductase]; Xref=Rhea:RHEA:69835, Rhea:RHEA-COMP:11964,
CC Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:28623, ChEBI:CHEBI:57618,
CC ChEBI:CHEBI:58210, ChEBI:CHEBI:72331;
CC Evidence={ECO:0000250|UniProtKB:Q5CCK3};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:69836;
CC Evidence={ECO:0000250|UniProtKB:Q5CCK3};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=campestanol + O2 + reduced [NADPH--hemoprotein reductase] = 6-
CC deoxycathasterone + H(+) + H2O + oxidized [NADPH--hemoprotein
CC reductase]; Xref=Rhea:RHEA:69831, Rhea:RHEA-COMP:11964, Rhea:RHEA-
CC COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:20714, ChEBI:CHEBI:36799, ChEBI:CHEBI:57618,
CC ChEBI:CHEBI:58210; Evidence={ECO:0000250|UniProtKB:Q5CCK3};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:69832;
CC Evidence={ECO:0000250|UniProtKB:Q5CCK3};
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413;
CC Evidence={ECO:0000250|UniProtKB:P04798};
CC -!- PATHWAY: Plant hormone biosynthesis; brassinosteroid biosynthesis.
CC {ECO:0000250|UniProtKB:Q5CCK3}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; CM000128; EEC74804.1; -; Genomic_DNA.
DR STRING; 39946.B8AJL3; -.
DR EnsemblPlants; BGIOSGA012169-TA; BGIOSGA012169-PA; BGIOSGA012169.
DR Gramene; BGIOSGA012169-TA; BGIOSGA012169-PA; BGIOSGA012169.
DR HOGENOM; CLU_001570_15_5_1; -.
DR OMA; FHWKLAA; -.
DR UniPathway; UPA00381; -.
DR Proteomes; UP000007015; Chromosome 3.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:EnsemblPlants.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0080132; F:fatty acid alpha-hydroxylase activity; IEA:EnsemblPlants.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0010012; F:steroid 22-alpha hydroxylase activity; IEA:EnsemblPlants.
DR GO; GO:0016132; P:brassinosteroid biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0009867; P:jasmonic acid mediated signaling pathway; IEA:EnsemblPlants.
DR GO; GO:0010358; P:leaf shaping; IEA:EnsemblPlants.
DR GO; GO:0009741; P:response to brassinosteroid; IEA:EnsemblPlants.
DR GO; GO:0009826; P:unidimensional cell growth; IEA:EnsemblPlants.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
PE 3: Inferred from homology;
KW Iron; Membrane; Metal-binding; Oxidoreductase; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..506
FT /note="Cytochrome P450 90B2"
FT /id="PRO_5002867622"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 449
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P04798"
SQ SEQUENCE 506 AA; 57345 MW; 39A855B2C11125B9 CRC64;
MAAMMASITS ELLFFLPFIL LALLTFYTTT VAKCHGGHWW RGGTTPAKRK RMNLPPGAAG
WPLVGETFGY LRAHPATSVG RFMEQHIARY GKIYRSSLFG ERTVVSADAG LNRYILQNEG
RLFECSYPRS IGGILGKWSM LVLVGDPHRE MRAISLNFLS SVRLRAVLLP EVERHTLLVL
RAWLPSSTFS AQHQAKKFTF NLMAKNIMSM DPGEEETERL RREYITFMKG VVSAPLNLPG
TPYWKALKSR AAILGVIERK MEERVEKLSK EDASVEQDDL LGWALKQSNL SKEQILDLLL
SLLFAGHETS SMALALAIFF LEGCPKAVQE LREEHLGIAR RQRLRGECKL SWEDYKEMVF
TQCVINETLR LGNVVRFLHR KVIKDVHYKG YDIPSGWKIL PVLAAVHLDS SLYEDPQRFN
PWRWKSSGSS GGLAQSSSFM PYGGGTRLCA GSELAKLEMA VFLHHLVLNF RWELAEPDQA
FVFPFVDFPK GLPIRVHRIA QDDEQE
