C90D2_ORYSI
ID C90D2_ORYSI Reviewed; 490 AA.
AC A2WLP4;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 73.
DE RecName: Full=Cytochrome P450 90D2 {ECO:0000250|UniProtKB:Q94IW5};
DE Short=OsCYP90D2 {ECO:0000250|UniProtKB:Q94IW5};
DE AltName: Full=3-dehydro-6-deoxoteasterone synthase {ECO:0000250|UniProtKB:Q94IW5};
DE EC=1.14.14.- {ECO:0000250|UniProtKB:Q94IW5};
DE AltName: Full=3-dehydroteasterone synthase {ECO:0000250|UniProtKB:Q94IW5};
DE EC=1.14.14.- {ECO:0000250|UniProtKB:Q94IW5};
GN Name=CYP90D2 {ECO:0000250|UniProtKB:Q94IW5};
GN ORFNames=OsI_00765 {ECO:0000312|EMBL:EAY72890.1};
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- FUNCTION: Catalyzes the C6-oxidation step in brassinosteroids
CC biosynthesis (By similarity). May convert 6-deoxoteasterone (6-deoxoTE)
CC to 3-dehydro-6-deoxoteasterone (6-deoxo3DT, 6-deoxo3DHT), and
CC teasterone (TE) to 3-dehydroteasterone (3DT, 3-DHT) (By similarity).
CC Involved in the elongation of leaf sheaths and stems (By similarity).
CC {ECO:0000250|UniProtKB:Q94IW5}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=6-deoxoteasterone + O2 + reduced [NADPH--hemoprotein
CC reductase] = 3-dehydro-6-deoxoteasterone + H(+) + 2 H2O + oxidized
CC [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:69983, Rhea:RHEA-
CC COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:20710,
CC ChEBI:CHEBI:20716, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210;
CC Evidence={ECO:0000250|UniProtKB:Q94IW5};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:69984;
CC Evidence={ECO:0000250|UniProtKB:Q94IW5};
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413;
CC Evidence={ECO:0000250|UniProtKB:P04798};
CC -!- PATHWAY: Plant hormone biosynthesis; brassinosteroid biosynthesis.
CC {ECO:0000250|UniProtKB:Q94IW5}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; CM000126; EAY72890.1; -; Genomic_DNA.
DR STRING; 39946.A2WLP4; -.
DR EnsemblPlants; BGIOSGA002945-TA; BGIOSGA002945-PA; BGIOSGA002945.
DR Gramene; BGIOSGA002945-TA; BGIOSGA002945-PA; BGIOSGA002945.
DR HOGENOM; CLU_001570_15_5_1; -.
DR OMA; DANHHES; -.
DR UniPathway; UPA00381; -.
DR Proteomes; UP000007015; Chromosome 1.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0016132; P:brassinosteroid biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 3: Inferred from homology;
KW Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..490
FT /note="Cytochrome P450 90D2"
FT /id="PRO_0000455307"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 437
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P04798"
SQ SEQUENCE 490 AA; 54843 MW; 84F37E5BCD50DFE1 CRC64;
MVSAAAGWAA PAFAVAAVVI WVVLCGELLR RRRRGAGSGK GDAAAAARLP PGSFGWPVVG
ETLEFVSCAY SPRPEAFVDK RRKLHGSAVF RSHLFGSATV VTADAEVSRF VLQSDARAFV
PWYPRSLTEL MGKSSILLIN GALQRRVHGL VGAFFKSSHL KSQLTADMRR RLSPALSSFP
DSSLLHVQHL AKSVVFEILV RGLIGLEAGE EMQQLKQQFQ EFIVGLMSLP IKLPGTRLYR
SLQAKKKMAR LIQRIIREKR ARRAAASLPR DAIDMLIGDG SDELTDELIS DNMIDLMIPA
EDSVPVLITL AVKFLSECPL ALHQLEEENI QLKRRKTDMG ETLQWTDYMS LSFTQHVITE
TLRLGNIIGG IMRKAVRDVE VKGHLIPKGW CVFVYFRSVH LDDTLYDEPY KFNPWRWKEK
DMSNGSFTPF GGGQRLCPGL DLARLEASIF LHHLVTSFRW VAEEDHIVNF PTVRLKRGMP
IRVTAKEDDD
