ACC4_CAEEL
ID ACC4_CAEEL Reviewed; 408 AA.
AC Q9U358;
DT 22-NOV-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Acetylcholine-gated ion channel acc-4 {ECO:0000305};
DE Flags: Precursor;
GN Name=acc-4 {ECO:0000303|PubMed:15579462, ECO:0000312|WormBase:T27E9.9};
GN ORFNames=T27E9.9 {ECO:0000312|WormBase:T27E9.9};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SUBUNIT.
RX PubMed=15579462; DOI=10.1074/jbc.m412644200;
RA Putrenko I., Zakikhani M., Dent J.A.;
RT "A family of acetylcholine-gated chloride channel subunits in
RT Caenorhabditis elegans.";
RL J. Biol. Chem. 280:6392-6398(2005).
RN [2] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP TISSUE SPECIFICITY.
RX PubMed=26705699; DOI=10.7554/elife.12432;
RA Pereira L., Kratsios P., Serrano-Saiz E., Sheftel H., Mayo A.E., Hall D.H.,
RA White J.G., LeBoeuf B., Garcia L.R., Alon U., Hobert O.;
RT "A cellular and regulatory map of the cholinergic nervous system of C.
RT elegans.";
RL Elife 4:12432-12477(2015).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=27782882; DOI=10.7554/elife.21734;
RA Takayanagi-Kiya S., Zhou K., Jin Y.;
RT "Release-dependent feedback inhibition by a presynaptically localized
RT ligand-gated anion channel.";
RL Elife 5:21734-21749(2016).
CC -!- FUNCTION: Probable acetylcholine-gated chloride channel subunit
CC (PubMed:15579462). Does not have ion channel activity alone as a
CC homopentamer, but forms a functional heteropentameric ion channel with
CC acc-1 (PubMed:15579462). May function with lgc-46 to regulate synaptic
CC transmission and synaptic vesicle release in response to acetylcholine
CC in cholinergic motor neurons (PubMed:27782882).
CC {ECO:0000269|PubMed:15579462, ECO:0000269|PubMed:27782882}.
CC -!- SUBUNIT: Does not form a functional homopentameric ion channel. Forms a
CC functional heteropentameric ion channel composed of acc-1 and acc-4.
CC May interact with acc-2; the interaction does not result in significant
CC heteropentameric ion channel activity. {ECO:0000269|PubMed:15579462}.
CC -!- SUBCELLULAR LOCATION: Presynaptic cell membrane
CC {ECO:0000269|PubMed:27782882}; Multi-pass membrane protein
CC {ECO:0000255}. Cell projection, axon {ECO:0000269|PubMed:27782882}.
CC -!- TISSUE SPECIFICITY: Expressed in all cholinergic motor neurons except
CC for ASJ and RIB neurons. Also expressed in AVF neurons.
CC {ECO:0000269|PubMed:26705699}.
CC -!- DISRUPTION PHENOTYPE: Grossly normal movement. In addition, in response
CC to induced acetylcholine release, there is prolonged and slow decay of
CC excitory postsynaptic currents which results in increased release of
CC synaptic vesicles during that late phase of synaptic vesicle release in
CC cholinergic motor neurons. Knockout in an lgc-46 gain of function
CC mutant background suppresses the slow locomotion defect in the single
CC lgc-46 gain of function mutant. Knockout together with gain of function
CC mutations in lgc-46 and acr-2 suppresses the convulsion defects in the
CC double lgc-46 and acr-2 double gain of function mutant.
CC {ECO:0000269|PubMed:27782882}.
CC -!- SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family.
CC {ECO:0000305}.
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DR EMBL; BX284603; CAB04879.2; -; Genomic_DNA.
DR PIR; E88613; E88613.
DR PIR; T25376; T25376.
DR RefSeq; NP_499789.1; NM_067388.4.
DR AlphaFoldDB; Q9U358; -.
DR SMR; Q9U358; -.
DR STRING; 6239.T27E9.9; -.
DR PaxDb; Q9U358; -.
DR EnsemblMetazoa; T27E9.9.1; T27E9.9.1; WBGene00012099.
DR EnsemblMetazoa; T27E9.9.2; T27E9.9.2; WBGene00012099.
DR GeneID; 176778; -.
DR UCSC; T27E9.9; c. elegans.
DR CTD; 176778; -.
DR WormBase; T27E9.9; CE24020; WBGene00012099; acc-4.
DR eggNOG; KOG3644; Eukaryota.
DR HOGENOM; CLU_010920_1_3_1; -.
DR InParanoid; Q9U358; -.
DR OMA; FYESFTH; -.
DR OrthoDB; 625651at2759; -.
DR PhylomeDB; Q9U358; -.
DR Reactome; R-CEL-977443; GABA receptor activation.
DR PRO; PR:Q9U358; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00012099; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0030424; C:axon; IEA:UniProtKB-SubCell.
DR GO; GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0042734; C:presynaptic membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0045202; C:synapse; IBA:GO_Central.
DR GO; GO:0005254; F:chloride channel activity; IEA:UniProtKB-KW.
DR GO; GO:0005231; F:excitatory extracellular ligand-gated ion channel activity; IBA:GO_Central.
DR GO; GO:0030594; F:neurotransmitter receptor activity; IBA:GO_Central.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; IEA:InterPro.
DR GO; GO:1904315; F:transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential; IBA:GO_Central.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:1902476; P:chloride transmembrane transport; IBA:GO_Central.
DR GO; GO:0034220; P:ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0050877; P:nervous system process; IBA:GO_Central.
DR GO; GO:0042391; P:regulation of membrane potential; IBA:GO_Central.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR Gene3D; 1.20.58.390; -; 1.
DR Gene3D; 2.70.170.10; -; 1.
DR InterPro; IPR006028; GABAA/Glycine_rcpt.
DR InterPro; IPR006202; Neur_chan_lig-bd.
DR InterPro; IPR036734; Neur_chan_lig-bd_sf.
DR InterPro; IPR006201; Neur_channel.
DR InterPro; IPR036719; Neuro-gated_channel_TM_sf.
DR InterPro; IPR038050; Neuro_actylchol_rec.
DR InterPro; IPR006029; Neurotrans-gated_channel_TM.
DR PANTHER; PTHR18945; PTHR18945; 1.
DR Pfam; PF02931; Neur_chan_LBD; 1.
DR Pfam; PF02932; Neur_chan_memb; 1.
DR PRINTS; PR00253; GABAARECEPTR.
DR PRINTS; PR00252; NRIONCHANNEL.
DR SUPFAM; SSF63712; SSF63712; 1.
DR SUPFAM; SSF90112; SSF90112; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Cell projection; Chloride; Chloride channel; Disulfide bond;
KW Glycoprotein; Ion channel; Ion transport; Ligand-gated ion channel;
KW Membrane; Reference proteome; Signal; Synapse; Transmembrane;
KW Transmembrane helix; Transport.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..408
FT /note="Acetylcholine-gated ion channel acc-4"
FT /evidence="ECO:0000305"
FT /id="PRO_5004333882"
FT TOPO_DOM 18..262
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 263..283
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 284..295
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 296..316
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 317..325
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 326..346
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 347..380
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 381..401
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 402..408
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT CARBOHYD 39
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 121
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 163
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 223
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 178..192
FT /evidence="ECO:0000250|UniProtKB:P02712"
SQ SEQUENCE 408 AA; 46218 MW; F2D7348DE2319081 CRC64;
MRLIILVISI LISNAGSLLR SLEDDSNECM FNCPKRARNV SVPHTEKTCM GDDAIIAQIL
DGYNKLDLPG GGHVVVSIEI WVQEVSKIIE ITSEFELDIY VTERWTDPSL AYSHLNPCKS
NMSVDGATIL NKIWNPHACF VNSKLANIHE SPFKNIFLQI YSNGSIWHNY RIKLTGPCSS
TLRTFPIDQQ RCMLFYESFT HNTDQVKMEW ITTVPPITIL KGNITLPDYV LVDFSSSSEL
RLYPPGIFNE LIATFTFQRL YGFYILQVYV PAYISVFISW VSFTLGAEQI PSRTTVGVNS
LLALTFQFGA VVNNLPKTSD VKAIDVWILS SMAFIFASLI ELAVVGYLSR DGQHGSIKCR
CSWLCMNCKD WTALKIDQMS SIVFPVSFLA FNIWYWFIFL GKLLVRTI
