UBIJ_SALTY
ID UBIJ_SALTY Reviewed; 201 AA.
AC Q9L6M5; Q7BM08;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Ubiquinone biosynthesis accessory factor UbiJ {ECO:0000255|HAMAP-Rule:MF_02215};
GN Name=ubiJ {ECO:0000255|HAMAP-Rule:MF_02215, ECO:0000303|PubMed:24142253};
GN Synonyms=yigP {ECO:0000312|EMBL:AAL22815.1};
GN OrderedLocusNames=STM3971 {ECO:0000312|EMBL:AAL22815.1};
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [2]
RP FUNCTION, PATHWAY, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 14028 / SGSC 2980 / CDC 6516-60 / NCTC 12023;
RX PubMed=24142253; DOI=10.1128/jb.01065-13;
RA Aussel L., Loiseau L., Hajj Chehade M., Pocachard B., Fontecave M.,
RA Pierrel F., Barras F.;
RT "ubiJ, a new gene required for aerobic growth and proliferation in
RT macrophage, is involved in coenzyme Q biosynthesis in Escherichia coli and
RT Salmonella enterica serovar Typhimurium.";
RL J. Bacteriol. 196:70-79(2014).
CC -!- FUNCTION: Required for ubiquinone (coenzyme Q) biosynthesis under
CC aerobic conditions (PubMed:24142253). Binds hydrophobic ubiquinone
CC biosynthetic intermediates via its SCP2 domain and is essential for the
CC stability of the Ubi complex (By similarity). May constitute a docking
CC platform where Ubi enzymes assemble and access their SCP2-bound
CC polyprenyl substrates (By similarity). Required for intracellular
CC proliferation in macrophages (PubMed:24142253).
CC {ECO:0000250|UniProtKB:P0ADP7, ECO:0000269|PubMed:24142253}.
CC -!- PATHWAY: Cofactor biosynthesis; ubiquinone biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_02215, ECO:0000269|PubMed:24142253}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02215}.
CC -!- DISRUPTION PHENOTYPE: During aerobic growth, mutant is impaired for
CC ubiquinone biosynthesis and for growth in rich medium, but it does not
CC present any defect under anaerobic conditions. Mutant is also impaired
CC for Salmonella intracellular proliferation in macrophages.
CC {ECO:0000269|PubMed:24142253}.
CC -!- SIMILARITY: Belongs to the UbiJ family. {ECO:0000255|HAMAP-
CC Rule:MF_02215, ECO:0000305}.
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DR EMBL; AF233324; AAF33421.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL22815.1; -; Genomic_DNA.
DR RefSeq; NP_462856.1; NC_003197.2.
DR RefSeq; WP_001116086.1; NC_003197.2.
DR AlphaFoldDB; Q9L6M5; -.
DR SMR; Q9L6M5; -.
DR STRING; 99287.STM3971; -.
DR PaxDb; Q9L6M5; -.
DR DNASU; 1255497; -.
DR EnsemblBacteria; AAL22815; AAL22815; STM3971.
DR GeneID; 1255497; -.
DR KEGG; stm:STM3971; -.
DR PATRIC; fig|99287.12.peg.4190; -.
DR HOGENOM; CLU_100130_2_0_6; -.
DR OMA; LELDWEY; -.
DR PhylomeDB; Q9L6M5; -.
DR BioCyc; SENT99287:STM3971-MON; -.
DR UniPathway; UPA00232; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0032150; P:ubiquinone biosynthetic process from chorismate; IBA:GO_Central.
DR HAMAP; MF_02215; UbiJ; 1.
DR InterPro; IPR003033; SCP2_sterol-bd_dom.
DR InterPro; IPR036527; SCP2_sterol-bd_dom_sf.
DR InterPro; IPR038989; UbiJ.
DR PANTHER; PTHR38693; PTHR38693; 1.
DR Pfam; PF02036; SCP2; 1.
DR SUPFAM; SSF55718; SSF55718; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Reference proteome; Ubiquinone biosynthesis.
FT CHAIN 1..201
FT /note="Ubiquinone biosynthesis accessory factor UbiJ"
FT /id="PRO_0000431477"
FT DOMAIN 15..112
FT /note="SCP2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02215"
SQ SEQUENCE 201 AA; 22143 MW; 2A868B61E1393843 CRC64;
MPFKPLVTAG IEGLLNTFLY RSPALKSART RLQGKVLCVK LKGFSMPLVL VFSERQVDVL
GAWEGEADCT VITQASVLPK LRDRQQLATL IRSGELEVQG DIQVVQNFVA LADLAEFDPA
ELLAPYTGDI AAESIGKVVR GGAKFLRHGF QRQQRYAAEA ITEEWRMAPG PLEVAWFAEE
TAAVERAVDS LTTRLEKLEA K
