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UBIK_ECOLI
ID   UBIK_ECOLI              Reviewed;          96 AA.
AC   Q46868; Q2M9F8;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 2.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=Ubiquinone biosynthesis accessory factor UbiK {ECO:0000255|HAMAP-Rule:MF_02216, ECO:0000305};
GN   Name=ubiK {ECO:0000255|HAMAP-Rule:MF_02216, ECO:0000303|PubMed:28559279};
GN   Synonyms=yqiC; OrderedLocusNames=b3042, JW5505;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [3]
RP   PROTEIN SEQUENCE OF 1-10.
RC   STRAIN=K12;
RX   PubMed=9868784; DOI=10.1111/j.1574-6968.1998.tb13343.x;
RA   Wasinger V.C., Humphery-Smith I.;
RT   "Small genes/gene-products in Escherichia coli K-12.";
RL   FEMS Microbiol. Lett. 169:375-382(1998).
RN   [4]
RP   FUNCTION, PATHWAY, SUBUNIT, INTERACTION WITH UBIJ, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=28559279; DOI=10.1074/jbc.m117.789164;
RA   Loiseau L., Fyfe C., Aussel L., Hajj Chehade M., Hernandez S.B., Faivre B.,
RA   Hamdane D., Mellot-Draznieks C., Rascalou B., Pelosi L., Velours C.,
RA   Cornu D., Lombard M., Casadesus J., Pierrel F., Fontecave M., Barras F.;
RT   "The UbiK protein is an accessory factor necessary for bacterial ubiquinone
RT   (UQ) biosynthesis and forms a complex with the UQ biogenesis factor UbiJ.";
RL   J. Biol. Chem. 292:11937-11950(2017).
RN   [5]
RP   FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX   PubMed=30686758; DOI=10.1016/j.chembiol.2018.12.001;
RA   Hajj Chehade M., Pelosi L., Fyfe C.D., Loiseau L., Rascalou B.,
RA   Brugiere S., Kazemzadeh K., Vo C.D., Ciccone L., Aussel L., Coute Y.,
RA   Fontecave M., Barras F., Lombard M., Pierrel F.;
RT   "A soluble metabolon synthesizes the isoprenoid lipid ubiquinone.";
RL   Cell Chem. Biol. 26:482-492(2019).
CC   -!- FUNCTION: Required for efficient ubiquinone (coenzyme Q) biosynthesis
CC       under aerobic conditions (PubMed:28559279, PubMed:30686758). UbiK is
CC       probably an accessory factor of Ubi enzymes and facilitates ubiquinone
CC       biosynthesis by acting as an assembly factor, a targeting factor, or
CC       both (PubMed:28559279). Dispensable for ubiquinone biosynthesis under
CC       anaerobiosis (PubMed:28559279). {ECO:0000269|PubMed:28559279,
CC       ECO:0000269|PubMed:30686758}.
CC   -!- PATHWAY: Cofactor biosynthesis; ubiquinone biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_02216, ECO:0000305|PubMed:28559279}.
CC   -!- SUBUNIT: Homotrimer (PubMed:28559279). Component of the Ubi complex
CC       metabolon, which regroups five ubiquinone biosynthesis proteins (UbiE,
CC       UbiF, UbiG, UbiH and UbiI) and two accessory factors (UbiK and the
CC       lipid-binding protein UbiJ) (PubMed:30686758). Interacts with the C-
CC       terminal part of UbiJ and forms a complex composed of 2 UbiK subunits
CC       and 1 UbiJ subunit. The UbiK-UbiJ complex interacts with palmitoleic
CC       acid (PubMed:28559279). {ECO:0000269|PubMed:28559279,
CC       ECO:0000269|PubMed:30686758}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02216,
CC       ECO:0000269|PubMed:30686758}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the gene impacts ubiquinone
CC       biosynthesis in aerobic conditions but does not decrease menaquinone
CC       biosynthesis. Mutant accumulates octaprenylphenol, an early
CC       intermediate of the ubiquinone biosynthetic pathway.
CC       {ECO:0000269|PubMed:28559279}.
CC   -!- SIMILARITY: Belongs to the UbiK family. {ECO:0000255|HAMAP-
CC       Rule:MF_02216, ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA69210.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; U28377; AAA69210.1; ALT_INIT; Genomic_DNA.
DR   EMBL; U00096; AAC76078.2; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77098.1; -; Genomic_DNA.
DR   PIR; H65091; H65091.
DR   RefSeq; NP_417514.4; NC_000913.3.
DR   RefSeq; WP_001295626.1; NZ_STEB01000001.1.
DR   AlphaFoldDB; Q46868; -.
DR   SMR; Q46868; -.
DR   BioGRID; 4259251; 14.
DR   BioGRID; 851841; 3.
DR   IntAct; Q46868; 3.
DR   STRING; 511145.b3042; -.
DR   jPOST; Q46868; -.
DR   PaxDb; Q46868; -.
DR   PRIDE; Q46868; -.
DR   EnsemblBacteria; AAC76078; AAC76078; b3042.
DR   EnsemblBacteria; BAE77098; BAE77098; BAE77098.
DR   GeneID; 947524; -.
DR   KEGG; ecj:JW5505; -.
DR   KEGG; eco:b3042; -.
DR   PATRIC; fig|511145.12.peg.3135; -.
DR   EchoBASE; EB2847; -.
DR   eggNOG; COG2960; Bacteria.
DR   HOGENOM; CLU_154412_0_2_6; -.
DR   InParanoid; Q46868; -.
DR   OMA; KQPNDVF; -.
DR   PhylomeDB; Q46868; -.
DR   BioCyc; EcoCyc:G7582-MON; -.
DR   BioCyc; MetaCyc:G7582-MON; -.
DR   UniPathway; UPA00232; -.
DR   PRO; PR:Q46868; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR   GO; GO:0016020; C:membrane; IDA:EcoCyc.
DR   GO; GO:0110142; C:ubiquinone biosynthesis complex; IDA:EcoCyc.
DR   GO; GO:0042802; F:identical protein binding; IDA:EcoCyc.
DR   GO; GO:0006744; P:ubiquinone biosynthetic process; IMP:EcoCyc.
DR   HAMAP; MF_02216; UbiK; 1.
DR   InterPro; IPR007475; UbiK.
DR   PANTHER; PTHR38040; PTHR38040; 1.
DR   Pfam; PF04380; BMFP; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Cytoplasm; Direct protein sequencing; Reference proteome;
KW   Ubiquinone biosynthesis.
FT   CHAIN           1..96
FT                   /note="Ubiquinone biosynthesis accessory factor UbiK"
FT                   /id="PRO_0000169417"
FT   COILED          59..86
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02216"
SQ   SEQUENCE   96 AA;  11276 MW;  393A8D90E7F6ACCC CRC64;
     MIDPKKIEQI ARQVHESMPK GIREFGEDVE KKIRQTLQAQ LTRLDLVSRE EFDVQTQVLL
     RTREKLALLE QRISELENRS TEIKKQPDPE TLPPTL
 
 
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