UBIK_ECOLI
ID UBIK_ECOLI Reviewed; 96 AA.
AC Q46868; Q2M9F8;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Ubiquinone biosynthesis accessory factor UbiK {ECO:0000255|HAMAP-Rule:MF_02216, ECO:0000305};
GN Name=ubiK {ECO:0000255|HAMAP-Rule:MF_02216, ECO:0000303|PubMed:28559279};
GN Synonyms=yqiC; OrderedLocusNames=b3042, JW5505;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP PROTEIN SEQUENCE OF 1-10.
RC STRAIN=K12;
RX PubMed=9868784; DOI=10.1111/j.1574-6968.1998.tb13343.x;
RA Wasinger V.C., Humphery-Smith I.;
RT "Small genes/gene-products in Escherichia coli K-12.";
RL FEMS Microbiol. Lett. 169:375-382(1998).
RN [4]
RP FUNCTION, PATHWAY, SUBUNIT, INTERACTION WITH UBIJ, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=28559279; DOI=10.1074/jbc.m117.789164;
RA Loiseau L., Fyfe C., Aussel L., Hajj Chehade M., Hernandez S.B., Faivre B.,
RA Hamdane D., Mellot-Draznieks C., Rascalou B., Pelosi L., Velours C.,
RA Cornu D., Lombard M., Casadesus J., Pierrel F., Fontecave M., Barras F.;
RT "The UbiK protein is an accessory factor necessary for bacterial ubiquinone
RT (UQ) biosynthesis and forms a complex with the UQ biogenesis factor UbiJ.";
RL J. Biol. Chem. 292:11937-11950(2017).
RN [5]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=30686758; DOI=10.1016/j.chembiol.2018.12.001;
RA Hajj Chehade M., Pelosi L., Fyfe C.D., Loiseau L., Rascalou B.,
RA Brugiere S., Kazemzadeh K., Vo C.D., Ciccone L., Aussel L., Coute Y.,
RA Fontecave M., Barras F., Lombard M., Pierrel F.;
RT "A soluble metabolon synthesizes the isoprenoid lipid ubiquinone.";
RL Cell Chem. Biol. 26:482-492(2019).
CC -!- FUNCTION: Required for efficient ubiquinone (coenzyme Q) biosynthesis
CC under aerobic conditions (PubMed:28559279, PubMed:30686758). UbiK is
CC probably an accessory factor of Ubi enzymes and facilitates ubiquinone
CC biosynthesis by acting as an assembly factor, a targeting factor, or
CC both (PubMed:28559279). Dispensable for ubiquinone biosynthesis under
CC anaerobiosis (PubMed:28559279). {ECO:0000269|PubMed:28559279,
CC ECO:0000269|PubMed:30686758}.
CC -!- PATHWAY: Cofactor biosynthesis; ubiquinone biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_02216, ECO:0000305|PubMed:28559279}.
CC -!- SUBUNIT: Homotrimer (PubMed:28559279). Component of the Ubi complex
CC metabolon, which regroups five ubiquinone biosynthesis proteins (UbiE,
CC UbiF, UbiG, UbiH and UbiI) and two accessory factors (UbiK and the
CC lipid-binding protein UbiJ) (PubMed:30686758). Interacts with the C-
CC terminal part of UbiJ and forms a complex composed of 2 UbiK subunits
CC and 1 UbiJ subunit. The UbiK-UbiJ complex interacts with palmitoleic
CC acid (PubMed:28559279). {ECO:0000269|PubMed:28559279,
CC ECO:0000269|PubMed:30686758}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02216,
CC ECO:0000269|PubMed:30686758}.
CC -!- DISRUPTION PHENOTYPE: Deletion of the gene impacts ubiquinone
CC biosynthesis in aerobic conditions but does not decrease menaquinone
CC biosynthesis. Mutant accumulates octaprenylphenol, an early
CC intermediate of the ubiquinone biosynthetic pathway.
CC {ECO:0000269|PubMed:28559279}.
CC -!- SIMILARITY: Belongs to the UbiK family. {ECO:0000255|HAMAP-
CC Rule:MF_02216, ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA69210.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U28377; AAA69210.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC76078.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE77098.1; -; Genomic_DNA.
DR PIR; H65091; H65091.
DR RefSeq; NP_417514.4; NC_000913.3.
DR RefSeq; WP_001295626.1; NZ_STEB01000001.1.
DR AlphaFoldDB; Q46868; -.
DR SMR; Q46868; -.
DR BioGRID; 4259251; 14.
DR BioGRID; 851841; 3.
DR IntAct; Q46868; 3.
DR STRING; 511145.b3042; -.
DR jPOST; Q46868; -.
DR PaxDb; Q46868; -.
DR PRIDE; Q46868; -.
DR EnsemblBacteria; AAC76078; AAC76078; b3042.
DR EnsemblBacteria; BAE77098; BAE77098; BAE77098.
DR GeneID; 947524; -.
DR KEGG; ecj:JW5505; -.
DR KEGG; eco:b3042; -.
DR PATRIC; fig|511145.12.peg.3135; -.
DR EchoBASE; EB2847; -.
DR eggNOG; COG2960; Bacteria.
DR HOGENOM; CLU_154412_0_2_6; -.
DR InParanoid; Q46868; -.
DR OMA; KQPNDVF; -.
DR PhylomeDB; Q46868; -.
DR BioCyc; EcoCyc:G7582-MON; -.
DR BioCyc; MetaCyc:G7582-MON; -.
DR UniPathway; UPA00232; -.
DR PRO; PR:Q46868; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0016020; C:membrane; IDA:EcoCyc.
DR GO; GO:0110142; C:ubiquinone biosynthesis complex; IDA:EcoCyc.
DR GO; GO:0042802; F:identical protein binding; IDA:EcoCyc.
DR GO; GO:0006744; P:ubiquinone biosynthetic process; IMP:EcoCyc.
DR HAMAP; MF_02216; UbiK; 1.
DR InterPro; IPR007475; UbiK.
DR PANTHER; PTHR38040; PTHR38040; 1.
DR Pfam; PF04380; BMFP; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Direct protein sequencing; Reference proteome;
KW Ubiquinone biosynthesis.
FT CHAIN 1..96
FT /note="Ubiquinone biosynthesis accessory factor UbiK"
FT /id="PRO_0000169417"
FT COILED 59..86
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02216"
SQ SEQUENCE 96 AA; 11276 MW; 393A8D90E7F6ACCC CRC64;
MIDPKKIEQI ARQVHESMPK GIREFGEDVE KKIRQTLQAQ LTRLDLVSRE EFDVQTQVLL
RTREKLALLE QRISELENRS TEIKKQPDPE TLPPTL