ID   UBIK_SALTY              Reviewed;         119 AA.
AC   Q8ZLY9;
DT   07-NOV-2018, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Ubiquinone biosynthesis accessory factor UbiK {ECO:0000255|HAMAP-Rule:MF_02216, ECO:0000305};
GN   Name=ubiK {ECO:0000255|HAMAP-Rule:MF_02216, ECO:0000303|PubMed:28559279};
GN   Synonyms=yqiC {ECO:0000303|PubMed:21554724};
GN   OrderedLocusNames=STM3196 {ECO:0000312|EMBL:AAL22070.1};
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
RN   [2]
RP   FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 14028 / SGSC 2980 / CDC 6516-60 / NCTC 12023;
RX   PubMed=21554724; DOI=10.1186/1471-2180-11-95;
RA   Carrica M.C., Craig P.O., Garcia-Angulo V.A., Aguirre A.,
RA   Garcia-Vescovi E., Goldbaum F.A., Cravero S.L.;
RT   "YqiC of Salmonella enterica serovar Typhimurium is a membrane fusogenic
RT   protein required for mice colonization.";
RL   BMC Microbiol. 11:95-95(2011).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=SL1344;
RX   PubMed=27777572; DOI=10.3389/fmicb.2016.01614;
RA   Wang K.C., Huang C.H., Ding S.M., Chen C.K., Fang H.W., Huang M.T.,
RA   Fang S.B.;
RT   "Role of yqiC in the pathogenicity of Salmonella and innate immune
RT   responses of human intestinal epithelium.";
RL   Front. Microbiol. 7:1614-1614(2016).
RN   [4]
RP   FUNCTION, PATHWAY, AND DISRUPTION PHENOTYPE.
RX   PubMed=28559279; DOI=10.1074/jbc.m117.789164;
RA   Loiseau L., Fyfe C., Aussel L., Hajj Chehade M., Hernandez S.B., Faivre B.,
RA   Hamdane D., Mellot-Draznieks C., Rascalou B., Pelosi L., Velours C.,
RA   Cornu D., Lombard M., Casadesus J., Pierrel F., Fontecave M., Barras F.;
RT   "The UbiK protein is an accessory factor necessary for bacterial ubiquinone
RT   (UQ) biosynthesis and forms a complex with the UQ biogenesis factor UbiJ.";
RL   J. Biol. Chem. 292:11937-11950(2017).
CC   -!- FUNCTION: Required for efficient ubiquinone (coenzyme Q) biosynthesis
CC       under aerobic conditions. UbiK is probably an accessory factor of Ubi
CC       enzymes and facilitates ubiquinone biosynthesis by acting as an
CC       assembly factor, a targeting factor, or both. Dispensable for
CC       ubiquinone biosynthesis under anaerobiosis (PubMed:28559279). Required
CC       for proliferation in macrophages and virulence in mice
CC       (PubMed:28559279, PubMed:21554724). Significantly contributes to
CC       colonization and invasion as well as host inflammation and innate
CC       immunity after infection (PubMed:27777572). In vitro, has membrane
CC       fusogenic activity at acidic pH (PubMed:21554724).
CC       {ECO:0000269|PubMed:21554724, ECO:0000269|PubMed:27777572,
CC       ECO:0000269|PubMed:28559279}.
CC   -!- PATHWAY: Cofactor biosynthesis; ubiquinone biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_02216, ECO:0000305|PubMed:28559279}.
CC   -!- SUBUNIT: Homotrimer. {ECO:0000269|PubMed:21554724}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02216,
CC       ECO:0000269|PubMed:21554724}. Note=Is both soluble and membrane
CC       associated inside the cell. {ECO:0000269|PubMed:21554724}.
CC   -!- DISRUPTION PHENOTYPE: Mutant is growth-deficient under aerobic
CC       conditions and is impaired to replicate at physiological and high
CC       temperatures (PubMed:21554724, PubMed:28559279). Mutant is defective
CC       for proliferation in macrophages and mice infection and has a severe
CC       attenuation in virulence in the murine model when inoculated both
CC       orally and intraperitoneally (PubMed:21554724, PubMed:28559279).
CC       Deletion inhibits colonization and invasion of four human cell lines,
CC       impairs flagella formation, activates the expression of type-1
CC       fimbriae-like structures on the cell surface, enhances biofilm
CC       formation and reduces bacterial motility. The deletion mutant is
CC       menaquinone-deficient (PubMed:27777572). {ECO:0000269|PubMed:21554724,
CC       ECO:0000269|PubMed:27777572, ECO:0000269|PubMed:28559279}.
CC   -!- SIMILARITY: Belongs to the UbiK family. {ECO:0000255|HAMAP-
CC       Rule:MF_02216, ECO:0000305}.
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DR   EMBL; AE006468; AAL22070.1; -; Genomic_DNA.
DR   RefSeq; NP_462111.3; NC_003197.2.
DR   RefSeq; WP_014343900.1; NC_003197.2.
DR   AlphaFoldDB; Q8ZLY9; -.
DR   SMR; Q8ZLY9; -.
DR   STRING; 99287.STM3196; -.
DR   PaxDb; Q8ZLY9; -.
DR   EnsemblBacteria; AAL22070; AAL22070; STM3196.
DR   GeneID; 1254719; -.
DR   KEGG; stm:STM3196; -.
DR   HOGENOM; CLU_154412_0_2_6; -.
DR   PhylomeDB; Q8ZLY9; -.
DR   BioCyc; SENT99287:STM3196-MON; -.
DR   UniPathway; UPA00232; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0006744; P:ubiquinone biosynthetic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_02216; UbiK; 1.
DR   InterPro; IPR007475; UbiK.
DR   PANTHER; PTHR38040; PTHR38040; 1.
DR   Pfam; PF04380; BMFP; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Cytoplasm; Reference proteome; Ubiquinone biosynthesis.
FT   CHAIN           1..119
FT                   /note="Ubiquinone biosynthesis accessory factor UbiK"
FT                   /id="PRO_0000445597"
FT   REGION          96..119
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          79..99
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02216"
SQ   SEQUENCE   119 AA;  13947 MW;  8226482F8CF67D9F CRC64;
     MASTYRTTIR ANTYQFRETT MIDPKKIEQI ARQVHESMPK GIREFGEDIE KKIRQTLQSQ
     LTRLDLVSRE EFDVQTQVLL RTREKLALLE QRLSELEARD KPEEVKPAPA IPPVDPQQE