ID   UBIT_ECOLI              Reviewed;         174 AA.
AC   P64599; P45474; Q2M953;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Ubiquinone biosynthesis accessory factor UbiT {ECO:0000255|HAMAP-Rule:MF_02231, ECO:0000305};
GN   Name=ubiT {ECO:0000255|HAMAP-Rule:MF_02231, ECO:0000303|PubMed:31289180};
GN   Synonyms=yhbT; OrderedLocusNames=b3157, JW3126;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [3]
RP   FUNCTION, PATHWAY, AND DISRUPTION PHENOTYPE.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=31289180; DOI=10.1128/mbio.01319-19;
RA   Pelosi L., Vo C.D., Abby S.S., Loiseau L., Rascalou B., Hajj Chehade M.,
RA   Faivre B., Gousse M., Chenal C., Touati N., Binet L., Cornu D., Fyfe C.D.,
RA   Fontecave M., Barras F., Lombard M., Pierrel F.;
RT   "Ubiquinone biosynthesis over the entire O2 range: characterization of a
RT   conserved O2-independent pathway.";
RL   MBio 10:E01319-E01319(2019).
CC   -!- FUNCTION: Required for O(2)-independent ubiquinone (coenzyme Q)
CC       biosynthesis. Likely functions as an accessory factor.
CC       {ECO:0000255|HAMAP-Rule:MF_02231, ECO:0000269|PubMed:31289180}.
CC   -!- PATHWAY: Cofactor biosynthesis; ubiquinone biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_02231, ECO:0000269|PubMed:31289180}.
CC   -!- DISRUPTION PHENOTYPE: Deletion mutant is unable to synthesize
CC       ubiquinone under strict anaerobic conditions. Mutant shows normal
CC       levels of ubiquinone after aerobic growth.
CC       {ECO:0000269|PubMed:31289180}.
CC   -!- SIMILARITY: Belongs to the UbiT family. {ECO:0000255|HAMAP-
CC       Rule:MF_02231, ECO:0000305}.
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DR   EMBL; U18997; AAA57960.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76191.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77203.1; -; Genomic_DNA.
DR   PIR; A65106; A65106.
DR   RefSeq; NP_417626.1; NC_000913.3.
DR   RefSeq; WP_001295552.1; NZ_SSUR01000002.1.
DR   AlphaFoldDB; P64599; -.
DR   SMR; P64599; -.
DR   BioGRID; 4259274; 170.
DR   BioGRID; 851982; 1.
DR   IntAct; P64599; 6.
DR   STRING; 511145.b3157; -.
DR   PaxDb; P64599; -.
DR   PRIDE; P64599; -.
DR   EnsemblBacteria; AAC76191; AAC76191; b3157.
DR   EnsemblBacteria; BAE77203; BAE77203; BAE77203.
DR   GeneID; 66672942; -.
DR   GeneID; 947669; -.
DR   KEGG; ecj:JW3126; -.
DR   KEGG; eco:b3157; -.
DR   PATRIC; fig|1411691.4.peg.3573; -.
DR   EchoBASE; EB2641; -.
DR   eggNOG; COG3154; Bacteria.
DR   HOGENOM; CLU_111894_1_0_6; -.
DR   InParanoid; P64599; -.
DR   OMA; RDIGLRW; -.
DR   PhylomeDB; P64599; -.
DR   BioCyc; EcoCyc:G7651-MON; -.
DR   UniPathway; UPA00232; -.
DR   PRO; PR:P64599; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR   GO; GO:0032150; P:ubiquinone biosynthetic process from chorismate; IMP:EcoCyc.
DR   Gene3D; 3.30.1050.10; -; 1.
DR   HAMAP; MF_02231; UbiT; 1.
DR   InterPro; IPR039543; POX18/UbiT/NSL-TP1.
DR   InterPro; IPR003033; SCP2_sterol-bd_dom.
DR   InterPro; IPR036527; SCP2_sterol-bd_dom_sf.
DR   InterPro; IPR016830; UbiT.
DR   PANTHER; PTHR10094; PTHR10094; 1.
DR   Pfam; PF02036; SCP2; 1.
DR   PIRSF; PIRSF025550; UCP025550_lpd_carrier; 1.
DR   SUPFAM; SSF55718; SSF55718; 1.
PE   3: Inferred from homology;
KW   Reference proteome; Ubiquinone biosynthesis.
FT   CHAIN           1..174
FT                   /note="Ubiquinone biosynthesis accessory factor UbiT"
FT                   /id="PRO_0000169456"
FT   DOMAIN          45..133
FT                   /note="SCP2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02231"
SQ   SEQUENCE   174 AA;  19672 MW;  64AF738130508204 CRC64;
     MLDKLRSRIV HLGPSLLSVP VKLTPFALKR QVLEQVLSWQ FRQALDDGEL EFLEGRWLSI
     HVRDIDLQWF TSVVNGKLVV SQNAQADVSF SADASDLLMI AARKQDPDTL FFQRRLVIEG
     DTELGLYVKN LMDAIELEQM PKALRMMLLQ LADFVEAGMK TAPETKQTSV GEPC