UBIX_CHLTR
ID UBIX_CHLTR Reviewed; 192 AA.
AC O84222;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Flavin prenyltransferase UbiX {ECO:0000255|HAMAP-Rule:MF_01984};
DE EC=2.5.1.129 {ECO:0000255|HAMAP-Rule:MF_01984};
GN Name=ubiX {ECO:0000255|HAMAP-Rule:MF_01984}; OrderedLocusNames=CT_220;
OS Chlamydia trachomatis (strain D/UW-3/Cx).
OC Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae;
OC Chlamydia/Chlamydophila group; Chlamydia.
OX NCBI_TaxID=272561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=D/UW-3/Cx;
RX PubMed=9784136; DOI=10.1126/science.282.5389.754;
RA Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L.,
RA Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W.;
RT "Genome sequence of an obligate intracellular pathogen of humans: Chlamydia
RT trachomatis.";
RL Science 282:754-759(1998).
CC -!- FUNCTION: Flavin prenyltransferase that catalyzes the synthesis of the
CC prenylated FMN cofactor (prenyl-FMN) for 4-hydroxy-3-polyprenylbenzoic
CC acid decarboxylase UbiD. The prenyltransferase is metal-independent and
CC links a dimethylallyl moiety from dimethylallyl monophosphate (DMAP) to
CC the flavin N5 and C6 atoms of FMN. {ECO:0000255|HAMAP-Rule:MF_01984}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dimethylallyl phosphate + FMNH2 = phosphate + prenyl-FMNH2;
CC Xref=Rhea:RHEA:37743, ChEBI:CHEBI:43474, ChEBI:CHEBI:57618,
CC ChEBI:CHEBI:87467, ChEBI:CHEBI:88052; EC=2.5.1.129;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01984};
CC -!- SIMILARITY: Belongs to the UbiX/PAD1 family. {ECO:0000255|HAMAP-
CC Rule:MF_01984}.
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DR EMBL; AE001273; AAC67812.1; -; Genomic_DNA.
DR PIR; C71543; C71543.
DR RefSeq; NP_219724.1; NC_000117.1.
DR RefSeq; WP_009871566.1; NC_000117.1.
DR PDB; 7KM2; X-ray; 2.19 A; A/B/C/D/E/F/G/H/I/J/K/L=1-192.
DR PDB; 7KM3; X-ray; 2.26 A; A/B/C/D/E/F/G/H/I/J/K/L=1-192.
DR PDBsum; 7KM2; -.
DR PDBsum; 7KM3; -.
DR AlphaFoldDB; O84222; -.
DR SMR; O84222; -.
DR STRING; 813.O172_01185; -.
DR EnsemblBacteria; AAC67812; AAC67812; CT_220.
DR GeneID; 884904; -.
DR KEGG; ctr:CT_220; -.
DR PATRIC; fig|272561.5.peg.235; -.
DR HOGENOM; CLU_074522_0_1_0; -.
DR InParanoid; O84222; -.
DR OMA; GATHIQD; -.
DR Proteomes; UP000000431; Chromosome.
DR GO; GO:0016831; F:carboxy-lyase activity; IBA:GO_Central.
DR GO; GO:0106141; F:flavin prenyltransferase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.1950; -; 1.
DR HAMAP; MF_01984; ubiX_pad; 1.
DR InterPro; IPR036551; Flavin_trans-like.
DR InterPro; IPR003382; Flavoprotein.
DR InterPro; IPR004507; UbiX-like.
DR PANTHER; PTHR43374; PTHR43374; 1.
DR Pfam; PF02441; Flavoprotein; 1.
DR SUPFAM; SSF52507; SSF52507; 1.
DR TIGRFAMs; TIGR00421; ubiX_pad; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Flavoprotein; FMN; Prenyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..192
FT /note="Flavin prenyltransferase UbiX"
FT /id="PRO_0000134961"
FT BINDING 10..12
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01984"
FT BINDING 36
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01984"
FT BINDING 92..95
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01984"
FT BINDING 127
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01984"
FT BINDING 157
FT /ligand="dimethylallyl phosphate"
FT /ligand_id="ChEBI:CHEBI:88052"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01984"
FT BINDING 173
FT /ligand="dimethylallyl phosphate"
FT /ligand_id="ChEBI:CHEBI:88052"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01984"
FT STRAND 3..8
FT /evidence="ECO:0007829|PDB:7KM2"
FT STRAND 10..12
FT /evidence="ECO:0007829|PDB:7KM3"
FT HELIX 14..26
FT /evidence="ECO:0007829|PDB:7KM2"
FT STRAND 30..35
FT /evidence="ECO:0007829|PDB:7KM2"
FT HELIX 37..40
FT /evidence="ECO:0007829|PDB:7KM2"
FT HELIX 53..55
FT /evidence="ECO:0007829|PDB:7KM2"
FT HELIX 58..63
FT /evidence="ECO:0007829|PDB:7KM2"
FT STRAND 64..67
FT /evidence="ECO:0007829|PDB:7KM2"
FT HELIX 75..77
FT /evidence="ECO:0007829|PDB:7KM2"
FT STRAND 85..91
FT /evidence="ECO:0007829|PDB:7KM2"
FT HELIX 93..101
FT /evidence="ECO:0007829|PDB:7KM2"
FT HELIX 107..117
FT /evidence="ECO:0007829|PDB:7KM2"
FT STRAND 122..126
FT /evidence="ECO:0007829|PDB:7KM2"
FT HELIX 133..144
FT /evidence="ECO:0007829|PDB:7KM2"
FT HELIX 163..177
FT /evidence="ECO:0007829|PDB:7KM2"
SQ SEQUENCE 192 AA; 20883 MW; 3CC252DC2137D9F5 CRC64;
MKRYVVGISG ASGIVLAVTL VSELARLGHH IDVIISPSAQ KTLYYELDTK SFLSTIPQNF
HNQIVLHHIS SIESSVSSGS NTIDATIIVP CSVATVAAIS CGLADNLLRR VADVALKEKR
PLILVPREAP LSAIHLENLL KLAQNGAVIL PPMPIWYFKP QTAEDIANDI VGKILAILQL
DSPLIKRWEN PR
