UBL3_HUMAN
ID UBL3_HUMAN Reviewed; 117 AA.
AC O95164; B2R4J1; Q5RL72; Q5VZS0; Q6FIG8; Q96SG7;
DT 13-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Ubiquitin-like protein 3;
DE AltName: Full=Membrane-anchored ubiquitin-fold protein;
DE Short=HsMUB;
DE Short=MUB;
DE AltName: Full=Protein HCG-1;
DE Flags: Precursor;
GN Name=UBL3; Synonyms=PNSC1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=10375635; DOI=10.1016/s0378-1119(99)00138-9;
RA Chadwick B.P., Kidd T., Sgouros J., Ish-Horowicz D., Frischauf A.-M.;
RT "Cloning, mapping and expression of UBL3, a novel ubiquitin-like gene.";
RL Gene 233:189-195(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=11230166; DOI=10.1101/gr.gr1547r;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J.,
RA Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W.,
RA Ottenwaelder B., Obermaier B., Tampe J., Heubner D., Wambutt R., Korn B.,
RA Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and analysis of
RT 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Corpus callosum, and Tongue;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057823; DOI=10.1038/nature02379;
RA Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L.,
RA Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S.,
RA Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P.,
RA Ambrose K.D., Andrews D.T., Ashwell R.I.S., Babbage A.K., Bagguley C.L.,
RA Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P.,
RA Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P.,
RA Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C.,
RA Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P.,
RA Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L.,
RA Frankish A.G., Frankland J., French L., Garner P., Garnett J.,
RA Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M.,
RA Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D.,
RA Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D.,
RA Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S.,
RA Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S.,
RA Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R.,
RA Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W.,
RA Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P.,
RA Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L.,
RA Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R.,
RA Rogers J., Ross M.T.;
RT "The DNA sequence and analysis of human chromosome 13.";
RL Nature 428:522-528(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [8]
RP IDENTIFICATION, NOMENCLATURE, PALMITOYLATION AT CYS-113, AND ISOPRENYLATION
RP AT CYS-114 IN VITRO.
RX PubMed=16831869; DOI=10.1074/jbc.m602283200;
RA Downes B.P., Saracco S.A., Lee S.S., Crowell D.N., Vierstra R.D.;
RT "MUBS: a family of ubiquitin-fold proteins that are plasma membrane-
RT anchored by prenylation.";
RL J. Biol. Chem. 281:27145-27157(2006).
RN [9]
RP STRUCTURE BY NMR OF 2-117.
RX PubMed=17567738; DOI=10.1110/ps.072834007;
RA de la Cruz N.B., Peterson F.C., Lytle B.L., Volkman B.F.;
RT "Solution structure of a membrane-anchored ubiquitin-fold (MUB) protein
RT from Homo sapiens.";
RL Protein Sci. 16:1479-1484(2007).
CC -!- INTERACTION:
CC O95164; Q9H8Y8: GORASP2; NbExp=3; IntAct=EBI-12876508, EBI-739467;
CC O95164; Q8WUI4-6: HDAC7; NbExp=3; IntAct=EBI-12876508, EBI-12094670;
CC O95164; P07686: HEXB; NbExp=3; IntAct=EBI-12876508, EBI-7133736;
CC O95164; Q8TC57: M1AP; NbExp=3; IntAct=EBI-12876508, EBI-748182;
CC O95164; Q9NWB1-5: RBFOX1; NbExp=3; IntAct=EBI-12876508, EBI-12123390;
CC O95164; P02766: TTR; NbExp=3; IntAct=EBI-12876508, EBI-711909;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Lipid-anchor
CC {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:10375635}.
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DR EMBL; AF044221; AAD02323.1; -; mRNA.
DR EMBL; AL080177; CAB45762.1; -; mRNA.
DR EMBL; AK311846; BAG34788.1; -; mRNA.
DR EMBL; AK056580; BAG51754.1; -; mRNA.
DR EMBL; CR533458; CAG38489.1; -; mRNA.
DR EMBL; AL158193; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL139188; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471075; EAX08446.1; -; Genomic_DNA.
DR EMBL; BC044582; AAH44582.1; -; mRNA.
DR EMBL; BC059385; AAH59385.1; -; mRNA.
DR CCDS; CCDS9334.1; -.
DR PIR; T12498; T12498.
DR RefSeq; NP_009037.1; NM_007106.3.
DR PDB; 2GOW; NMR; -; A=2-117.
DR PDBsum; 2GOW; -.
DR AlphaFoldDB; O95164; -.
DR BMRB; O95164; -.
DR SMR; O95164; -.
DR BioGRID; 111413; 18.
DR IntAct; O95164; 8.
DR STRING; 9606.ENSP00000370055; -.
DR iPTMnet; O95164; -.
DR PhosphoSitePlus; O95164; -.
DR SwissPalm; O95164; -.
DR BioMuta; UBL3; -.
DR EPD; O95164; -.
DR jPOST; O95164; -.
DR MassIVE; O95164; -.
DR MaxQB; O95164; -.
DR PaxDb; O95164; -.
DR PeptideAtlas; O95164; -.
DR PRIDE; O95164; -.
DR ProteomicsDB; 50678; -.
DR Antibodypedia; 22737; 166 antibodies from 25 providers.
DR DNASU; 5412; -.
DR Ensembl; ENST00000380680.5; ENSP00000370055.4; ENSG00000122042.10.
DR GeneID; 5412; -.
DR KEGG; hsa:5412; -.
DR MANE-Select; ENST00000380680.5; ENSP00000370055.4; NM_007106.4; NP_009037.1.
DR UCSC; uc001usp.4; human.
DR CTD; 5412; -.
DR DisGeNET; 5412; -.
DR GeneCards; UBL3; -.
DR HGNC; HGNC:12504; UBL3.
DR HPA; ENSG00000122042; Low tissue specificity.
DR MIM; 604711; gene.
DR neXtProt; NX_O95164; -.
DR OpenTargets; ENSG00000122042; -.
DR PharmGKB; PA37151; -.
DR VEuPathDB; HostDB:ENSG00000122042; -.
DR eggNOG; ENOG502RYGN; Eukaryota.
DR GeneTree; ENSGT00390000004952; -.
DR HOGENOM; CLU_151471_0_0_1; -.
DR InParanoid; O95164; -.
DR OMA; EWESDRV; -.
DR OrthoDB; 1555986at2759; -.
DR PhylomeDB; O95164; -.
DR TreeFam; TF314489; -.
DR PathwayCommons; O95164; -.
DR SignaLink; O95164; -.
DR BioGRID-ORCS; 5412; 10 hits in 1062 CRISPR screens.
DR ChiTaRS; UBL3; human.
DR EvolutionaryTrace; O95164; -.
DR GeneWiki; UBL3; -.
DR GenomeRNAi; 5412; -.
DR Pharos; O95164; Tbio.
DR PRO; PR:O95164; -.
DR Proteomes; UP000005640; Chromosome 13.
DR RNAct; O95164; protein.
DR Bgee; ENSG00000122042; Expressed in inferior olivary complex and 208 other tissues.
DR ExpressionAtlas; O95164; baseline and differential.
DR Genevisible; O95164; HS.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR017000; MUB.
DR InterPro; IPR000626; Ubiquitin-like_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR InterPro; IPR040015; UBL3-like.
DR InterPro; IPR039540; UBL3-like_ubiquitin_dom.
DR PANTHER; PTHR13169; PTHR13169; 1.
DR Pfam; PF13881; Rad60-SLD_2; 1.
DR PIRSF; PIRSF032572; MUB; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS50053; UBIQUITIN_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Lipoprotein; Membrane; Methylation; Palmitate;
KW Prenylation; Reference proteome.
FT CHAIN 1..114
FT /note="Ubiquitin-like protein 3"
FT /id="PRO_0000114862"
FT PROPEP 115..117
FT /note="Removed in mature form"
FT /evidence="ECO:0000305"
FT /id="PRO_0000248183"
FT DOMAIN 10..88
FT /note="Ubiquitin-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00214"
FT MOD_RES 114
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000305"
FT LIPID 113
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000305|PubMed:16831869"
FT LIPID 114
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000305|PubMed:16831869"
FT CONFLICT 10
FT /note="I -> V (in Ref. 4; CAG38489)"
FT /evidence="ECO:0000305"
FT CONFLICT 28
FT /note="P -> H (in Ref. 7; AAH44582)"
FT /evidence="ECO:0000305"
FT STRAND 10..15
FT /evidence="ECO:0007829|PDB:2GOW"
FT STRAND 21..26
FT /evidence="ECO:0007829|PDB:2GOW"
FT HELIX 32..40
FT /evidence="ECO:0007829|PDB:2GOW"
FT HELIX 55..57
FT /evidence="ECO:0007829|PDB:2GOW"
FT STRAND 58..66
FT /evidence="ECO:0007829|PDB:2GOW"
FT HELIX 74..76
FT /evidence="ECO:0007829|PDB:2GOW"
FT STRAND 82..90
FT /evidence="ECO:0007829|PDB:2GOW"
FT STRAND 95..99
FT /evidence="ECO:0007829|PDB:2GOW"
SQ SEQUENCE 117 AA; 13157 MW; 4EB1375D9C0B029F CRC64;
MSSNVPADMI NLRLILVSGK TKEFLFSPND SASDIAKHVY DNWPMDWEEE QVSSPNILRL
IYQGRFLHGN VTLGALKLPF GKTTVMHLVA RETLPEPNSQ GQRNREKTGE SNCCVIL
