ID   UBL4A_RABIT             Reviewed;         157 AA.
AC   B7NZQ9;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=Ubiquitin-like protein 4A;
GN   Name=UBL4A;
OS   Oryctolagus cuniculus (Rabbit).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Lagomorpha; Leporidae; Oryctolagus.
OX   NCBI_TaxID=9986;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Antonellis A., Ayele K., Benjamin B., Blakesley R.W., Boakye A.,
RA   Bouffard G.G., Brinkley C., Brooks S., Chu G., Coleman H., Engle J.,
RA   Gestole M., Greene A., Guan X., Gupta J., Haghighi P., Han J., Hansen N.,
RA   Ho S.-L., Hu P., Hunter G., Hurle B., Idol J.R., Kwong P., Laric P.,
RA   Larson S., Lee-Lin S.-Q., Legaspi R., Madden M., Maduro Q.L., Maduro V.B.,
RA   Margulies E.H., Masiello C., Maskeri B., McDowell J., Mojidi H.A.,
RA   Mullikin J.C., Oestreicher J.S., Park M., Portnoy M.E., Prasad A., Puri O.,
RA   Reddix-Dugue N., Schandler K., Schueler M.G., Sison C., Stantripop S.,
RA   Stephen E., Taye A., Thomas J.W., Thomas P.J., Tsipouri V., Ung L.,
RA   Vogt J.L., Wetherby K.D., Young A., Green E.D.;
RT   "NISC comparative sequencing initiative.";
RL   Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: As part of a cytosolic protein quality control complex, the
CC       BAG6/BAT3 complex, maintains misfolded and hydrophobic patches-
CC       containing proteins in a soluble state and participates in their proper
CC       delivery to the endoplasmic reticulum or alternatively can promote
CC       their sorting to the proteasome where they undergo degradation. The
CC       BAG6/BAT3 complex is involved in the post-translational delivery of
CC       tail-anchored/type II transmembrane proteins to the endoplasmic
CC       reticulum membrane. Recruited to ribosomes, it interacts with the
CC       transmembrane region of newly synthesized tail-anchored proteins and
CC       together with SGTA and ASNA1 mediates their delivery to the endoplasmic
CC       reticulum. Client proteins that cannot be properly delivered to the
CC       endoplasmic reticulum are ubiquitinated and sorted to the proteasome.
CC       Similarly, the BAG6/BAT3 complex also functions as a sorting platform
CC       for proteins of the secretory pathway that are mislocalized to the
CC       cytosol either delivering them to the proteasome for degradation or to
CC       the endoplasmic reticulum. The BAG6/BAT3 complex also plays a role in
CC       the endoplasmic reticulum-associated degradation (ERAD), a quality
CC       control mechanism that eliminates unwanted proteins of the endoplasmic
CC       reticulum through their retrotranslocation to the cytosol and their
CC       targeting to the proteasome. It maintains these retrotranslocated
CC       proteins in an unfolded yet soluble state condition in the cytosol to
CC       ensure their proper delivery to the proteasome.
CC       {ECO:0000250|UniProtKB:P11441}.
CC   -!- SUBUNIT: Component of the BAG6/BAT3 complex, at least composed of BAG6,
CC       UBL4A and GET4/TRC35. Interacts with BAG6; the interaction is direct
CC       and required for UBL4A protein stability. Interacts with USP13; may be
CC       indirect via BAG6. {ECO:0000250|UniProtKB:P11441}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:P11441}. Nucleus {ECO:0000250|UniProtKB:P11441}.
CC   -!- PTM: Polyubiquitinated. Ubiquitination by AMFR and deubiquitination by
CC       USP13 may regulate the interaction between the BAG6/BAT3 complex and
CC       SGTA and therefore may regulate client proteins fate.
CC       {ECO:0000250|UniProtKB:P11441}.
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DR   EMBL; DP001061; ACK44284.1; -; Genomic_DNA.
DR   RefSeq; NP_001164852.1; NM_001171381.1.
DR   AlphaFoldDB; B7NZQ9; -.
DR   BMRB; B7NZQ9; -.
DR   SMR; B7NZQ9; -.
DR   BioGRID; 1173367; 3.
DR   STRING; 9986.ENSOCUP00000024993; -.
DR   Ensembl; ENSOCUT00000023480; ENSOCUP00000024993; ENSOCUG00000022433.
DR   GeneID; 100328764; -.
DR   KEGG; ocu:100328764; -.
DR   CTD; 8266; -.
DR   eggNOG; KOG0001; Eukaryota.
DR   GeneTree; ENSGT00950000182808; -.
DR   HOGENOM; CLU_119809_0_0_1; -.
DR   InParanoid; B7NZQ9; -.
DR   OMA; SMDTSYM; -.
DR   OrthoDB; 1586605at2759; -.
DR   Proteomes; UP000001811; Unplaced.
DR   Bgee; ENSOCUG00000022433; Expressed in skeletal muscle tissue and 17 other tissues.
DR   GO; GO:0071818; C:BAT3 complex; ISS:UniProtKB.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0071816; P:tail-anchored membrane protein insertion into ER membrane; ISS:UniProtKB.
DR   CDD; cd01807; Ubl_UBL4A_like; 1.
DR   InterPro; IPR000626; Ubiquitin-like_dom.
DR   InterPro; IPR029071; Ubiquitin-like_domsf.
DR   InterPro; IPR019954; Ubiquitin_CS.
DR   InterPro; IPR019956; Ubiquitin_dom.
DR   InterPro; IPR041421; Ubl4_C_TUGS.
DR   InterPro; IPR044724; Ubl_UBL4A-like.
DR   Pfam; PF17840; Tugs; 1.
DR   Pfam; PF00240; ubiquitin; 1.
DR   PRINTS; PR00348; UBIQUITIN.
DR   SMART; SM00213; UBQ; 1.
DR   SUPFAM; SSF54236; SSF54236; 1.
DR   PROSITE; PS00299; UBIQUITIN_1; 1.
DR   PROSITE; PS50053; UBIQUITIN_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome;
KW   Transport; Ubl conjugation.
FT   CHAIN           1..157
FT                   /note="Ubiquitin-like protein 4A"
FT                   /id="PRO_0000403738"
FT   DOMAIN          1..76
FT                   /note="Ubiquitin-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00214"
FT   REGION          96..138
FT                   /note="Required and sufficient for interaction with BAG6"
FT                   /evidence="ECO:0000250|UniProtKB:P11441"
FT   MOD_RES         90
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P11441"
FT   CROSSLNK        48
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in ubiquitin)"
FT                   /evidence="ECO:0000250|UniProtKB:P11441"
SQ   SEQUENCE   157 AA;  17632 MW;  F68A7C657DE5AE51 CRC64;
     MQLTVKALQG RECNLQVPED EQVSTLKQLV SEKLNVPVRQ QRLLFKGKAL ADGKRLSDYS
     IGPNSKLNLV VKPLEKVLLA EGTARRLADS PPPPVWHLIS KVLARHFSAA DASRVLEQLQ
     RDYERSLSRL TLDDIERLAS RFLHPEGTEA MEKGLSK