UBN1_MOUSE
ID UBN1_MOUSE Reviewed; 1135 AA.
AC Q4G0F8; Q3UUZ4; Q6P9K7; Q8BNC3; Q9CRM4; Q9CS40;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 30-AUG-2005, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Ubinuclein-1;
DE AltName: Full=Ubiquitously expressed nuclear protein;
GN Name=Ubn1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 1-197 (ISOFORM 1/2), AND NUCLEOTIDE SEQUENCE [LARGE
RP SCALE MRNA] OF 557-1135 (ISOFORM 3).
RC STRAIN=C57BL/6J;
RC TISSUE=Embryo, Placenta, Spinal ganglion, Vagina, and Wolffian duct;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 70-1135 (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=18823282; DOI=10.1042/bc20080072;
RA Aho S., Lupo J., Coly P.-A., Sabine A., Castellazzi M., Morand P.,
RA Sergeant A., Manet E., Boyer V., Gruffat H.;
RT "Characterization of the ubinuclein protein as a new member of the nuclear
RT and adhesion complex components (NACos).";
RL Biol. Cell 101:319-334(2009).
CC -!- FUNCTION: Acts as a novel regulator of senescence. Involved in the
CC formation of senescence-associated heterochromatin foci (SAHF), which
CC represses expression of proliferation-promoting genes. Binds to
CC proliferation-promoting genes. May be required for replication-
CC independent chromatin assembly (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of a complex that includes at least ASF1A, CABIN1,
CC HIRA, histone H3.3 and UBN1. Interacts with HIRA (via WD repeat
CC domain); the interaction is direct. Interacts with ASF1A, CEBPA, TJP1,
CC TJP2 and TJP3 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm {ECO:0000250}. Nucleus, PML
CC body {ECO:0000250}. Cell junction, tight junction
CC {ECO:0000269|PubMed:18823282}. Note=Localized as a nuclear speckled-
CC like pattern in proliferating primary fibroblasts. Colocalizes with
CC HIRA, PML and SP100 in PML bodies of senescent cells. Colocalizes with
CC CLDN1. Detected along the upper granular cell layer of epidermis. When
CC overexpressed, accumulates in the nucleus in cells showing defective
CC cytokinesis (By similarity). Colocalizes with TJP1. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q4G0F8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q4G0F8-2; Sequence=VSP_036972;
CC Name=3;
CC IsoId=Q4G0F8-3; Sequence=VSP_036973;
CC -!- TISSUE SPECIFICITY: Expressed in bile canaliculi in liver, in
CC bronchiolar epithelium in lung and in tubular structures of gland ducts
CC inside the olfactory epithelium and tongue epithelium.
CC {ECO:0000269|PubMed:18823282}.
CC -!- SIMILARITY: Belongs to the ubinuclein family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EDK97277.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AK019173; BAB31587.1; -; mRNA.
DR EMBL; AK020146; BAB32010.1; -; mRNA.
DR EMBL; AK084046; BAC39106.1; -; mRNA.
DR EMBL; AK137727; BAE23479.1; -; mRNA.
DR EMBL; AK147571; BAE28001.1; -; mRNA.
DR EMBL; CH466521; EDK97277.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BC060723; AAH60723.1; -; mRNA.
DR EMBL; BC098372; AAH98372.1; -; mRNA.
DR CCDS; CCDS27931.1; -. [Q4G0F8-1]
DR CCDS; CCDS88873.1; -. [Q4G0F8-2]
DR RefSeq; NP_080942.1; NM_026666.3. [Q4G0F8-1]
DR RefSeq; XP_006521879.1; XM_006521816.3. [Q4G0F8-1]
DR RefSeq; XP_006521880.1; XM_006521817.3. [Q4G0F8-1]
DR RefSeq; XP_006521882.1; XM_006521819.3.
DR RefSeq; XP_017172375.1; XM_017316886.1. [Q4G0F8-1]
DR AlphaFoldDB; Q4G0F8; -.
DR SMR; Q4G0F8; -.
DR BioGRID; 228360; 2.
DR STRING; 10090.ENSMUSP00000061843; -.
DR iPTMnet; Q4G0F8; -.
DR PhosphoSitePlus; Q4G0F8; -.
DR EPD; Q4G0F8; -.
DR jPOST; Q4G0F8; -.
DR MaxQB; Q4G0F8; -.
DR PaxDb; Q4G0F8; -.
DR PeptideAtlas; Q4G0F8; -.
DR PRIDE; Q4G0F8; -.
DR ProteomicsDB; 298355; -. [Q4G0F8-1]
DR ProteomicsDB; 298356; -. [Q4G0F8-2]
DR ProteomicsDB; 298357; -. [Q4G0F8-3]
DR Antibodypedia; 11234; 162 antibodies from 35 providers.
DR DNASU; 170644; -.
DR Ensembl; ENSMUST00000052449; ENSMUSP00000061843; ENSMUSG00000039473. [Q4G0F8-1]
DR Ensembl; ENSMUST00000229126; ENSMUSP00000155263; ENSMUSG00000039473. [Q4G0F8-2]
DR Ensembl; ENSMUST00000230703; ENSMUSP00000155223; ENSMUSG00000039473. [Q4G0F8-1]
DR GeneID; 170644; -.
DR KEGG; mmu:170644; -.
DR UCSC; uc007ybq.1; mouse. [Q4G0F8-1]
DR UCSC; uc007ybr.1; mouse. [Q4G0F8-2]
DR UCSC; uc007ybs.1; mouse. [Q4G0F8-3]
DR CTD; 29855; -.
DR MGI; MGI:1891307; Ubn1.
DR VEuPathDB; HostDB:ENSMUSG00000039473; -.
DR eggNOG; KOG4786; Eukaryota.
DR GeneTree; ENSGT00940000158857; -.
DR HOGENOM; CLU_007400_0_0_1; -.
DR InParanoid; Q4G0F8; -.
DR OMA; HQDPEPA; -.
DR OrthoDB; 469344at2759; -.
DR PhylomeDB; Q4G0F8; -.
DR TreeFam; TF326088; -.
DR Reactome; R-MMU-2559584; Formation of Senescence-Associated Heterochromatin Foci (SAHF).
DR BioGRID-ORCS; 170644; 4 hits in 74 CRISPR screens.
DR ChiTaRS; Ubn1; mouse.
DR PRO; PR:Q4G0F8; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q4G0F8; protein.
DR Bgee; ENSMUSG00000039473; Expressed in lacrimal gland and 244 other tissues.
DR ExpressionAtlas; Q4G0F8; baseline and differential.
DR Genevisible; Q4G0F8; MM.
DR GO; GO:0005923; C:bicellular tight junction; IDA:UniProtKB.
DR GO; GO:0034451; C:centriolar satellite; ISO:MGI.
DR GO; GO:0016604; C:nuclear body; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0016605; C:PML body; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006336; P:DNA replication-independent chromatin assembly; ISO:MGI.
DR GO; GO:0030216; P:keratinocyte differentiation; TAS:MGI.
DR InterPro; IPR014840; HRD.
DR InterPro; IPR026936; Ubinuclein-1.
DR InterPro; IPR026947; UBN_middle_dom.
DR PANTHER; PTHR21669:SF12; PTHR21669:SF12; 1.
DR Pfam; PF08729; HUN; 1.
DR Pfam; PF14075; UBN_AB; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Alternative splicing; Cell junction; Chromatin regulator;
KW Coiled coil; DNA-binding; Nucleus; Phosphoprotein; Reference proteome;
KW Tight junction.
FT CHAIN 1..1135
FT /note="Ubinuclein-1"
FT /id="PRO_0000370691"
FT REGION 1..38
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 77..96
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 171..220
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 253..280
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 480..503
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 603..646
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 747..985
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1094..1135
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 476..539
FT /evidence="ECO:0000255"
FT COMPBIAS 1..17
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 199..219
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 253..270
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 615..646
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 807..841
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 857..961
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 166
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9NPG3"
FT MOD_RES 173
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NPG3"
FT MOD_RES 175
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NPG3"
FT MOD_RES 222
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q9NPG3"
FT MOD_RES 323
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NPG3"
FT MOD_RES 336
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NPG3"
FT MOD_RES 338
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NPG3"
FT MOD_RES 493
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NPG3"
FT MOD_RES 660
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NPG3"
FT MOD_RES 677
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NPG3"
FT MOD_RES 1028
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NPG3"
FT VAR_SEQ 1090..1120
FT /note="SLLAGLHSSPPHTAPLPHAAVSTHVPQSLPD -> N (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_036972"
FT VAR_SEQ 1091..1135
FT /note="LLAGLHSSPPHTAPLPHAAVSTHVPQSLPDASQLHGKGPVVPRKL -> KFL
FT SPASVPMLCAMLGPVCVWCNHGNIPHLWKLHSTRDSCFVPDWSAGCLGL (in
FT isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_036973"
FT CONFLICT 184
FT /note="K -> N (in Ref. 1; BAB31587)"
FT /evidence="ECO:0000305"
FT CONFLICT 681
FT /note="A -> G (in Ref. 1; BAC39106)"
FT /evidence="ECO:0000305"
FT CONFLICT 801
FT /note="G -> A (in Ref. 1; BAC39106)"
FT /evidence="ECO:0000305"
FT CONFLICT 969
FT /note="A -> P (in Ref. 1; BAC39106)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1135 AA; 122336 MW; 8B5994B86F97E0F7 CRC64;
MSEPHRVQFT SVPGSLNPAF LKKSRKEEVG GTEQHQDCEP AAAAVRITLT LFEPDHKRCP
EFFYPELVKN IRGKVKGLHP GDKKKDVLDP FNDEEKERHK VEALARKFEE KYGGKKRRKD
RIQDLIDMGY GYDESDSFID NSEAYDELVP ASLTTKYGGF YINSGTLQFR QASESEDDFI
KEKKKKSPKK RKLKEGGEKI KKKKKDDTYD KEKKSKKSKF SKAGFTALNA SKEKKKKKYS
GSLSVREMLK KFQKEKEAQK KREEEHKPVA VSSIEAQGLR ELEGTSDPLL SLFGSTSDND
LLQAATAMDS LTDLDLEQLL SESPEGSPFR DMDDGSDSLG VGLDQEFRQP SSFPEGLPIP
LEKRVKELAQ AARAAEGESK QKFFTQDING ILLDIEVQTR ELTSQIRSGV FAYLASFLPC
SKDALVKRAR KLHLYEQGGR LKEPLQKLKD AIGRAMPEQV AKYQDECQAH TQAKVAKMLE
EEKDKEQRER ICSDEEEDEE KGGRRIMGPR KKFQWNDEIR ELLCQVVKIK LESRDLERNS
KAQAWEDCVK AFLDAEVKPL WPKGWMQART LFKESRRGHG HLTSLLAKKK VIAPSKIKMK
ESSVKLDKKV SVPSGQHGGP TTLLSEHQGG GLNTGANSRE HPSQATCGLT DSVSVTLEDS
LDEDLVRNPA SSVDAVSKEL ATLNSRAANS SEFTLPTPSK APTEKVGGVL CTEEKRNFAK
PSSSAPPPTN ALQSPLNFLA EQALALGQSS QEKKPEGSGF KELSCQGPLS KGVPELHPSK
AKHHNLPRTS HGPQAAAPVP GPQVKVFHAG TQQQKSFTPP SPFVNKLQGP KATSPQCHRS
LLQLVKTAAK GQAFHATMPA SSGSSPASSS SAHKTTASNS TTISHPAKLH PTSSVGPSYK
NNPFAGSVSK HGASSSSPSP GGGAQVQSSV SGASLPGVQS PSAGQSASRA APSSAVKKTP
VTQKLTLVAP PGGPNGDSGG GTQGVAKLLT SSLKPAAVSS VTSSTSLPKG TGGAVLLSNT
SSLSLLSSSY KSNNPKLPGA MNSNSLGIIT QFPLHVLSFN ADSSAKAGVS KDAIVTGPAP
GTFHHGLSHS LLAGLHSSPP HTAPLPHAAV STHVPQSLPD ASQLHGKGPV VPRKL
