UBN2_ARATH
ID UBN2_ARATH Reviewed; 717 AA.
AC F4I700; O80664; Q9FVX7; Q9ZT59;
DT 25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 69.
DE RecName: Full=Ubinuclein-2 {ECO:0000303|PubMed:25086063};
DE Short=AtUBN2 {ECO:0000303|PubMed:25086063};
DE AltName: Full=Ubiquitously expressed nuclear protein 2 {ECO:0000303|PubMed:25086063};
GN Name=UBN2 {ECO:0000303|PubMed:25086063}; Synonyms=G1 {ECO:0000303|Ref.3};
GN OrderedLocusNames=At1g77310 {ECO:0000312|Araport:AT1G77310};
GN ORFNames=F2P24.2 {ECO:0000312|EMBL:AAG29192.1},
GN T14N5.16 {ECO:0000312|EMBL:AAC34359.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 518-717.
RC STRAIN=cv. Wassilewskija;
RX AGRICOLA=IND21959123; DOI=10.1007/s004970050142;
RA He C., Mascarenhas J.P.;
RT "MEI1, an Arabidopsis gene required for male meiosis: isolation and
RT characterization.";
RL Sex. Plant Reprod. 11:199-207(1998).
RN [4]
RP SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, SUBUNIT, INTERACTION WITH HIRA,
RP GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=25086063; DOI=10.1242/bio.20148680;
RA Nie X., Wang H., Li J., Holec S., Berger F.;
RT "The HIRA complex that deposits the histone H3.3 is conserved in
RT Arabidopsis and facilitates transcriptional dynamics.";
RL Biol. Open 3:794-802(2014).
RN [5]
RP SUBUNIT, AND GENE FAMILY.
RX PubMed=25600486; DOI=10.1111/tpj.12758;
RA Duc C., Benoit M., Le Goff S., Simon L., Poulet A., Cotterell S.,
RA Tatout C., Probst A.V.;
RT "The histone chaperone complex HIR maintains nucleosome occupancy and
RT counterbalances impaired histone deposition in CAF-1 complex mutants.";
RL Plant J. 81:707-722(2015).
CC -!- FUNCTION: May be required for replication-independent chromatin
CC assembly. {ECO:0000250|UniProtKB:Q9NPG3}.
CC -!- SUBUNIT: Component of the HIRA complex made of UBN1, UBN2, ASF1A,
CC CABIN1 and HIRA (PubMed:25086063, PubMed:25600486). Interacts with HIRA
CC (PubMed:25086063). {ECO:0000269|PubMed:25086063,
CC ECO:0000269|PubMed:25600486}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768,
CC ECO:0000269|PubMed:25086063}. Nucleus, nucleolus
CC {ECO:0000269|PubMed:25086063}. Note=Localized at rDNA loci in the
CC nucleolus. {ECO:0000269|PubMed:25086063}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype.
CC {ECO:0000269|PubMed:25086063}.
CC -!- SIMILARITY: Belongs to the ubinuclein family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC34359.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAG29192.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC004260; AAC34359.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC078898; AAG29192.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE35962.1; -; Genomic_DNA.
DR EMBL; AF074849; AAD02829.1; -; Genomic_DNA.
DR PIR; A96802; A96802.
DR PIR; T00459; T00459.
DR RefSeq; NP_177855.4; NM_106380.7.
DR AlphaFoldDB; F4I700; -.
DR STRING; 3702.AT1G77310.1; -.
DR PaxDb; F4I700; -.
DR PRIDE; F4I700; -.
DR ProteomicsDB; 233046; -.
DR EnsemblPlants; AT1G77310.1; AT1G77310.1; AT1G77310.
DR GeneID; 844067; -.
DR Gramene; AT1G77310.1; AT1G77310.1; AT1G77310.
DR KEGG; ath:AT1G77310; -.
DR Araport; AT1G77310; -.
DR TAIR; locus:2196010; AT1G77310.
DR eggNOG; ENOG502QRNW; Eukaryota.
DR HOGENOM; CLU_016217_1_0_1; -.
DR InParanoid; F4I700; -.
DR OMA; NEMVNQP; -.
DR OrthoDB; 741874at2759; -.
DR PRO; PR:F4I700; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4I700; baseline and differential.
DR GO; GO:0005730; C:nucleolus; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0030875; C:rDNA protrusion; IDA:UniProtKB.
DR GO; GO:0006336; P:DNA replication-independent chromatin assembly; IBA:GO_Central.
DR InterPro; IPR014840; HRD.
DR Pfam; PF08729; HUN; 1.
PE 1: Evidence at protein level;
KW Chromatin regulator; Nucleus; Reference proteome.
FT CHAIN 1..717
FT /note="Ubinuclein-2"
FT /id="PRO_0000441877"
FT REGION 114..136
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 166..308
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 620..717
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 634..641
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 121..136
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 187..208
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 214..250
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 259..274
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 281..308
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 646..662
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 677..701
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 702..717
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 545
FT /note="H -> D (in Ref. 3; AAD02829)"
FT /evidence="ECO:0000305"
FT CONFLICT 595
FT /note="T -> A (in Ref. 3; AAD02829)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 717 AA; 79143 MW; 61DB791E003CB16B CRC64;
MEDEPKLPTD DGPTFNESCK ISSEILTAGD RKLLKVELLK EETTLVSWKK LMDEASKENG
GLFVSAPERL LNANPNLEFR LAPGAQTENE MVNQPHPNRL NSVIAKIERL YMGKDGSDGE
ELDGAPDDDD YDTEDSFIDD AELDEYFEVD NSPIKHDGFF VNRGKLERIE PSATSNQQQP
KKRRRKESAK PCGDVVDVSR KRAKMAKTAG GKDQSASPGP SSKKISNDSK TVQDSFSPLK
AQNGNDSLVL ENVKHTDKAN HQPMNATSPK SKAAGSSGPL HPKCSSKSVH EQSNSPPGKS
RPNVSAKSAV VRQQVNNGMP DLDIATESKT SIQISKKSGS NGRPKYSTLE KAIRNLEKLV
AESRPPAATE NQDADISSQA VKRGLPGDVK LHLAKVARIA YASQGEISGE LINRLMGIVG
HLIQIRSLKR NLKIMIDSIV TANREKDTRF QRIKSEITEM LKTQVPLVES QETNQEAGTS
DDFQDVGSLG KSPVKKFVMD VALEEKLCDL YDVFVEGMDE HSGSQIRKLY SDLAQLWPNS
LVDNHEIRRA ICREKERRRA LEGNIGKEMD QTKITKKKQT QLVPKSEGIT YPDKTSGVEV
KASVVLTATT TSLVDCQPAA DSSFERSKQQ HEKLKRTSSL SNPAAEGKKV RRKTEPALEE
THLPAEKPLV LALKRQTHLK SKTHKQVQVH PQSKAHKQAQ VHPKAKTQTP PDLNLPS
