ID   C97B1_PEA               Reviewed;         552 AA.
AC   Q43078;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Cytochrome P450 97B1, chloroplastic;
DE            EC=1.14.-.-;
DE   AltName: Full=Cytochrome P450 97A2;
DE   Flags: Precursor;
GN   Name=CYP97B1; Synonyms=CYP97A2;
OS   Pisum sativum (Garden pea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Kleine Rheinlaenderin; TISSUE=Seedling;
RA   Baltrusch M., Fulda M., Wolter F.-P., Heinz E.;
RT   "Cloning and sequencing of a cytochrome P450 from Pisum sativum L.";
RL   (er) Plant Gene Register PGR97-106(1997).
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; Z49263; CAA89260.1; -; mRNA.
DR   PIR; S71163; S71163.
DR   AlphaFoldDB; Q43078; -.
DR   SMR; Q43078; -.
DR   GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; Heme; Iron; Membrane; Metal-binding; Monooxygenase;
KW   Oxidoreductase; Plastid; Transit peptide.
FT   TRANSIT         1..52
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           53..552
FT                   /note="Cytochrome P450 97B1, chloroplastic"
FT                   /id="PRO_0000052194"
FT   BINDING         528
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   552 AA;  62281 MW;  131B2FE0CE035E84 CRC64;
     MVAAPISTVK LTDANLHTRF HSSSSSTPST LSLPLSLHFH FSSHSKRFSS IRCQSVNGEK
     RKQSSRNVFD NASNLLTSLL SGANLGSMPI AEGAVTDLFD RPLFFSLYDW FLEHGSVYKL
     AFGPKAFVVV SDPIVARHIL RENAFSYDKG VLADILEPIM GKGLIPADLE TWKQRRRVIA
     PGFHTSYLEA MVQLFTSCSE RTVLKVNELL EGEGRDGQKS VELDLEAEFS NLALEIIGLG
     VFNYDFGSVT NESPVIKAVY GTLFEAEHRS TFYIPYWKFP LARWIVPRQR KFQDDLKVIN
     TCLDGLIRNA KESRQETDVE KLQQRDYSNL KDASLLRFLV DMRGVDVDDR QLRDDLMTML
     IAGHETTAAV LTWAVFLLAQ NPDKMKKAQA EVDLVLGMGK PTFELLKKLE YIRLIVVETL
     RLYPQPPLLI RRSLKPDVLP GGHKGDKDGY TIPAGTDVFI SVYNLHRSPY FWDRPNDFEP
     ERFLVQNNNE EVEGWAGFDP SRSPGALYPN EIISDFAFLP FGGGPRKCVG DQFALMESTV
     ALVCCYRISM WN