ID   C97B2_SOYBN             Reviewed;         576 AA.
AC   O48921;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Cytochrome P450 97B2, chloroplastic;
DE            EC=1.14.-.-;
DE   Flags: Precursor;
GN   Name=CYP97B2;
OS   Glycine max (Soybean) (Glycine hispida).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC   Glycine subgen. Soja.
OX   NCBI_TaxID=3847;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Siminszky B., Dewey R.E., Corbin F.T.;
RL   Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; AF022457; AAB94586.1; -; mRNA.
DR   PIR; T05904; T05904.
DR   RefSeq; NP_001235534.1; NM_001248605.1.
DR   AlphaFoldDB; O48921; -.
DR   SMR; O48921; -.
DR   STRING; 3847.GLYMA11G01860.1; -.
DR   PRIDE; O48921; -.
DR   GeneID; 606504; -.
DR   KEGG; gmx:606504; -.
DR   eggNOG; KOG0157; Eukaryota.
DR   HOGENOM; CLU_001570_5_5_1; -.
DR   InParanoid; O48921; -.
DR   OrthoDB; 1247045at2759; -.
DR   Proteomes; UP000008827; Unplaced.
DR   Genevisible; O48921; GM.
DR   GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; Heme; Iron; Membrane; Metal-binding; Monooxygenase;
KW   Oxidoreductase; Plastid; Reference proteome; Transit peptide.
FT   TRANSIT         1..44
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           45..576
FT                   /note="Cytochrome P450 97B2, chloroplastic"
FT                   /id="PRO_0000052195"
FT   BINDING         519
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   576 AA;  64741 MW;  A07558368CB2A05F CRC64;
     MSVDTSSTLS TVTDANLHSR FHSRLVPFTH HFSLSQPKRI SSIRCQSINT DKKKSSRNLL
     GNASNLLTDL LSGGSIGSMP IAEGAVSDLL GRPLFFSLYD WFLEHGAVYK LAFGPKAFVV
     VSDPIVARHI LRENAFSYDK GVLADILEPI MGKGLIPADL DTWKQRRRVI APAFHNSYLE
     AMVKIFTTCS ERTILKFNKL LEGEGYDGPD SIELDLEAEF SSLALDIIGL GVFNYDFGSV
     TKESPVIKAV YGTLFEAEHR STFYIPYWKI PLARWIVPRQ RKFQDDLKVI NTCLDGLIRN
     AKESRQETDV EKLQQRDYLN LKDASLLRFL VDMRGADVDD RQLRDDLMTM LIAGHETTAA
     VLTWAVFLLA QNPSKMKKAQ AEVDLVLGTG RPTFESLKEL QYIRLIVVEA LRLYPQPPLL
     IRRSLKSDVL PGGHKGEKDG YAIPAGTDVF ISVYNLHRSP YFWDRPDDFE PERFLVQNKN
     EEIEGWAGLD PSRSPGALYP NEVISDFAFL PFGGGPRKCV GDQFALMEST VALTMLLQNF
     DVELKGTPES VELVTGATIH TKNGMWCRLK KRSNLR