UBP50_HUMAN
ID UBP50_HUMAN Reviewed; 339 AA.
AC Q70EL3; E9PP86;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Inactive ubiquitin carboxyl-terminal hydrolase 50;
DE AltName: Full=Inactive ubiquitin-specific peptidase 50;
GN Name=USP50;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, AND LACK OF
RP ENZYME ACTIVITY.
RX PubMed=14715245; DOI=10.1016/j.bbrc.2003.12.050;
RA Quesada V., Diaz-Perales A., Gutierrez-Fernandez A., Garabaya C., Cal S.,
RA Lopez-Otin C.;
RT "Cloning and enzymatic analysis of 22 novel human ubiquitin-specific
RT proteases.";
RL Biochem. Biophys. Res. Commun. 314:54-62(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16572171; DOI=10.1038/nature04601;
RA Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K.,
RA Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K.,
RA FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N.,
RA Abouelleil A., Arachchi H.M., Baradarani L., Birditt B., Bloom S.,
RA Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K.,
RA DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J.,
RA Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E.,
RA Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B.,
RA Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R.,
RA O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B.,
RA Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S.,
RA Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.;
RT "Analysis of the DNA sequence and duplication history of human chromosome
RT 15.";
RL Nature 440:671-675(2006).
RN [3]
RP IDENTIFICATION.
RX PubMed=15060002; DOI=10.1101/gr.1946304;
RA Puente X.S., Lopez-Otin C.;
RT "A genomic analysis of rat proteases and protease inhibitors.";
RL Genome Res. 14:609-622(2004).
CC -!- FUNCTION: Has no peptidase activity.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q70EL3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q70EL3-2; Sequence=VSP_054248;
CC -!- TISSUE SPECIFICITY: Weakly expressed in a few tissues.
CC {ECO:0000269|PubMed:14715245}.
CC -!- MISCELLANEOUS: [Isoform 1]: Splicing events through non-canonical
CC splice sites. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the peptidase C19 family. {ECO:0000305}.
CC -!- CAUTION: Although the active site residues are conserved, lacks the
CC conserved Asp/Asn residue which is normally found 7-9 residues after
CC the catalytic His. {ECO:0000305}.
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DR EMBL; AJ583818; CAE47745.2; -; mRNA.
DR EMBL; BN000339; CAE48395.1; -; mRNA.
DR EMBL; AC012170; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS53944.1; -. [Q70EL3-2]
DR RefSeq; NP_987090.2; NM_203494.4. [Q70EL3-2]
DR AlphaFoldDB; Q70EL3; -.
DR SMR; Q70EL3; -.
DR BioGRID; 131884; 30.
DR IntAct; Q70EL3; 21.
DR STRING; 9606.ENSP00000434676; -.
DR MEROPS; C19.058; -.
DR iPTMnet; Q70EL3; -.
DR PhosphoSitePlus; Q70EL3; -.
DR BioMuta; USP50; -.
DR DMDM; 74723115; -.
DR PaxDb; Q70EL3; -.
DR PeptideAtlas; Q70EL3; -.
DR PRIDE; Q70EL3; -.
DR ProteomicsDB; 22642; -.
DR ProteomicsDB; 68548; -. [Q70EL3-1]
DR Antibodypedia; 24751; 129 antibodies from 25 providers.
DR DNASU; 373509; -.
DR Ensembl; ENST00000532404.6; ENSP00000434676.1; ENSG00000170236.16. [Q70EL3-2]
DR GeneID; 373509; -.
DR KEGG; hsa:373509; -.
DR MANE-Select; ENST00000532404.6; ENSP00000434676.1; NM_203494.5; NP_987090.2. [Q70EL3-2]
DR UCSC; uc021sky.2; human. [Q70EL3-1]
DR CTD; 373509; -.
DR DisGeNET; 373509; -.
DR GeneCards; USP50; -.
DR HGNC; HGNC:20079; USP50.
DR HPA; ENSG00000170236; Tissue enhanced (testis).
DR neXtProt; NX_Q70EL3; -.
DR OpenTargets; ENSG00000170236; -.
DR VEuPathDB; HostDB:ENSG00000170236; -.
DR eggNOG; KOG1868; Eukaryota.
DR GeneTree; ENSGT00940000160441; -.
DR HOGENOM; CLU_008279_1_3_1; -.
DR InParanoid; Q70EL3; -.
DR OrthoDB; 1283205at2759; -.
DR PhylomeDB; Q70EL3; -.
DR TreeFam; TF106277; -.
DR PathwayCommons; Q70EL3; -.
DR SignaLink; Q70EL3; -.
DR BioGRID-ORCS; 373509; 11 hits in 1068 CRISPR screens.
DR ChiTaRS; USP50; human.
DR GenomeRNAi; 373509; -.
DR Pharos; Q70EL3; Tbio.
DR PRO; PR:Q70EL3; -.
DR Proteomes; UP000005640; Chromosome 15.
DR RNAct; Q70EL3; protein.
DR Bgee; ENSG00000170236; Expressed in left testis and 93 other tissues.
DR ExpressionAtlas; Q70EL3; baseline and differential.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0043197; C:dendritic spine; IBA:GO_Central.
DR GO; GO:0031313; C:extrinsic component of endosome membrane; IBA:GO_Central.
DR GO; GO:0019897; C:extrinsic component of plasma membrane; IBA:GO_Central.
DR GO; GO:0030496; C:midbody; IBA:GO_Central.
DR GO; GO:0014069; C:postsynaptic density; IBA:GO_Central.
DR GO; GO:0004843; F:cysteine-type deubiquitinase activity; IBA:GO_Central.
DR GO; GO:0019783; F:ubiquitin-like protein peptidase activity; IDA:MGI.
DR GO; GO:0007032; P:endosome organization; IBA:GO_Central.
DR GO; GO:0035063; P:nuclear speck organization; IMP:MGI.
DR GO; GO:2001056; P:positive regulation of cysteine-type endopeptidase activity; IMP:MGI.
DR GO; GO:0032731; P:positive regulation of interleukin-1 beta production; IMP:MGI.
DR GO; GO:0032741; P:positive regulation of interleukin-18 production; IEA:Ensembl.
DR GO; GO:1900227; P:positive regulation of NLRP3 inflammasome complex assembly; IMP:MGI.
DR GO; GO:0016579; P:protein deubiquitination; IDA:MGI.
DR GO; GO:0071108; P:protein K48-linked deubiquitination; IBA:GO_Central.
DR GO; GO:0070536; P:protein K63-linked deubiquitination; IBA:GO_Central.
DR GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR001394; Peptidase_C19_UCH.
DR InterPro; IPR018200; USP_CS.
DR InterPro; IPR028889; USP_dom.
DR Pfam; PF00443; UCH; 1.
DR SUPFAM; SSF54001; SSF54001; 1.
DR PROSITE; PS00972; USP_1; 1.
DR PROSITE; PS00973; USP_2; 1.
DR PROSITE; PS50235; USP_3; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Reference proteome.
FT CHAIN 1..339
FT /note="Inactive ubiquitin carboxyl-terminal hydrolase 50"
FT /id="PRO_0000249527"
FT DOMAIN 44..339
FT /note="USP"
FT VAR_SEQ 83..88
FT /note="KFLLPS -> N (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_054248"
SQ SEQUENCE 339 AA; 38955 MW; B41D707864CBB5AC CRC64;
MTSQPSLPAD DFDIYHVLAE CTDYYDTLPV KEADGNQPHF QGVTGLWNLG NTCCVNAISQ
CLCSILPLVE YFLTGKYITA LQKFLLPSDC SEVATAFAYL MTDMWLGDSD CVSPEIFWSA
LGNLYPAFTK KMQQDAQEFL ICVLNELHEA LKKYHYSRRR SYEKGSTQRC CRKWITTETS
IITQLFEEQL NYSIVCLKCE KCTYKNEVFT VFSLPIPSKY ECSLRDCLQC FFQQDALTWN
NEIHCSFCET KQETAVRASI SKAPKIIIFH LKRFDIQGTT KRKLRTDIHY PLTNLDLTPY
ICSIFRKYPK YNLCAVVNHF GDLDGGHYTA FCKNSVTQA
