UBP50_MACFA
ID UBP50_MACFA Reviewed; 373 AA.
AC Q4R6D3;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Putative ubiquitin carboxyl-terminal hydrolase 50;
DE EC=3.4.19.12;
DE AltName: Full=Deubiquitinating enzyme 50;
DE AltName: Full=Ubiquitin thioesterase 50;
DE AltName: Full=Ubiquitin-specific-processing protease 50;
GN Name=USP50; ORFNames=QtsA-18237;
OS Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC Cercopithecidae; Cercopithecinae; Macaca.
OX NCBI_TaxID=9541;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RG International consortium for macaque cDNA sequencing and analysis;
RT "DNA sequences of macaque genes expressed in brain or testis and its
RT evolutionary implications.";
RL Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May recognize and hydrolyze the peptide bond at the C-
CC terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin
CC precursors as well as that of ubiquitinated proteins (By similarity).
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide
CC and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-
CC residue protein attached to proteins as an intracellular targeting
CC signal).; EC=3.4.19.12;
CC -!- MISCELLANEOUS: The human ortholog is inactive.
CC -!- SIMILARITY: Belongs to the peptidase C19 family. {ECO:0000305}.
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DR EMBL; AB169252; BAE01342.1; -; mRNA.
DR RefSeq; NP_001270928.1; NM_001283999.1.
DR AlphaFoldDB; Q4R6D3; -.
DR SMR; Q4R6D3; -.
DR STRING; 9541.XP_005559593.1; -.
DR MEROPS; C19.058; -.
DR GeneID; 101865081; -.
DR CTD; 373509; -.
DR VEuPathDB; HostDB:ENSMFAG00000043824; -.
DR eggNOG; KOG1868; Eukaryota.
DR OMA; ECTDHYD; -.
DR Proteomes; UP000233100; Chromosome 7.
DR GO; GO:0004843; F:cysteine-type deubiquitinase activity; IEA:UniProtKB-EC.
DR GO; GO:0016579; P:protein deubiquitination; IEA:InterPro.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR001394; Peptidase_C19_UCH.
DR InterPro; IPR018200; USP_CS.
DR InterPro; IPR028889; USP_dom.
DR Pfam; PF00443; UCH; 1.
DR SUPFAM; SSF54001; SSF54001; 1.
DR PROSITE; PS00972; USP_1; 1.
DR PROSITE; PS00973; USP_2; 1.
DR PROSITE; PS50235; USP_3; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Protease; Reference proteome; Thiol protease;
KW Ubl conjugation pathway.
FT CHAIN 1..373
FT /note="Putative ubiquitin carboxyl-terminal hydrolase 50"
FT /id="PRO_0000249528"
FT DOMAIN 44..364
FT /note="USP"
FT ACT_SITE 53
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10092,
FT ECO:0000255|PROSITE-ProRule:PRU10093"
FT ACT_SITE 322
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10092,
FT ECO:0000255|PROSITE-ProRule:PRU10093"
SQ SEQUENCE 373 AA; 42752 MW; 09C80C792C8E4FE4 CRC64;
MTSQRSLPAD DFGIYYVLAE CTDYYDTLPV KEADGSQPCS QGVTGLRNLG NTCYMNAILQ
CLCSISPLVE YFLSGKYITA LQNDCSEVAT AFAYLMTDMW LGDSDCVSPE IFRSALGNLY
PAFTKKTQQD AQEFLIYVLN ELHEALKKYH YPRRRSHEKG SAQRCCRKWI TTETSVITQL
FEGQLNYSIV CLKCEKCTYK NEVFTVLSLP IPSEYECSLQ DCLQCFFQQD TLTWNNQIHC
SFCETKQETA VRAGISKAPK IIIFHLKRFD IQGTTKRKLR TDIHYPLTNL DLTPYICPIF
RKYPKYNLCA VVNHFGDLDG GHYTAFCKNS FTQAWYSFDD TRVSEIPDTS VQNATAYLLF
YSCQPFSIPI QKH
