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UBS3A_MOUSE
ID   UBS3A_MOUSE             Reviewed;         624 AA.
AC   Q3V3E1; Q3TED7;
DT   25-JAN-2012, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2005, sequence version 1.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=Ubiquitin-associated and SH3 domain-containing protein A;
DE   AltName: Full=Suppressor of T-cell receptor signaling 2;
DE            Short=STS-2;
DE   AltName: Full=T-cell ubiquitin ligand 1;
DE            Short=TULA-1;
GN   Name=Ubash3a; ORFNames=Sts2;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J, and NOD; TISSUE=Thymus;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [3]
RP   LACK OF PROTEIN PHOSPHATASE ACTIVITY.
RX   PubMed=20670933; DOI=10.1074/jbc.m110.114181;
RA   Chen X., Ren L., Kim S., Carpino N., Daniel J.L., Kunapuli S.P.,
RA   Tsygankov A.Y., Pei D.;
RT   "Determination of the substrate specificity of protein-tyrosine phosphatase
RT   TULA-2 and identification of Syk as a TULA-2 substrate.";
RL   J. Biol. Chem. 285:31268-31276(2010).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 354-622, FUNCTION, AND VERY LOW
RP   PHOSPHATASE ACTIVITY AT PH 5.
RX   PubMed=19196006; DOI=10.1021/bi802219n;
RA   Chen Y., Jakoncic J., Carpino N., Nassar N.;
RT   "Structural and functional characterization of the 2H-phosphatase domain of
RT   Sts-2 reveals an acid-dependent phosphatase activity.";
RL   Biochemistry 48:1681-1690(2009).
CC   -!- FUNCTION: Interferes with CBL-mediated down-regulation and degradation
CC       of receptor-type tyrosine kinases. Promotes accumulation of activated
CC       target receptors, such as T-cell receptors, EGFR and PDGFRB, on the
CC       cell surface. May inhibit dynamin-dependent endocytic pathways by
CC       functionally sequestering dynamin via its SH3 domain (By similarity).
CC       Exhibits negligigle protein tyrosine phosphatase activity at neutral
CC       pH. May act as a dominant-negative regulator of UBASH3B-dependent
CC       dephosphorylation. {ECO:0000250, ECO:0000269|PubMed:19196006}.
CC   -!- SUBUNIT: Homodimer or homooligomer. Interacts with CBL. Part of a
CC       complex containing CBL and activated EGFR. Interacts with ubiquitin and
CC       with mono-ubiquitinated proteins. Interacts with dynamin (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC   -!- MISCELLANEOUS: Has very nearly lost all enzyme activity. Has very low,
CC       but measurable tyrosine phosphatase activity at pH 5.0.
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DR   EMBL; AK041690; BAE20600.1; -; mRNA.
DR   EMBL; AK169696; BAE41311.1; -; mRNA.
DR   EMBL; AC154652; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC167247; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CU024900; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   CCDS; CCDS50054.1; -.
DR   RefSeq; NP_808491.2; NM_177823.4.
DR   PDB; 3D4I; X-ray; 1.95 A; A/B/C/D=354-622.
DR   PDB; 3D6A; X-ray; 2.25 A; A/B/C/D=354-622.
DR   PDB; 3DB1; X-ray; 2.77 A; A/B/C/D=354-622.
DR   PDBsum; 3D4I; -.
DR   PDBsum; 3D6A; -.
DR   PDBsum; 3DB1; -.
DR   AlphaFoldDB; Q3V3E1; -.
DR   SMR; Q3V3E1; -.
DR   IntAct; Q3V3E1; 6.
DR   STRING; 10090.ENSMUSP00000045890; -.
DR   iPTMnet; Q3V3E1; -.
DR   PhosphoSitePlus; Q3V3E1; -.
DR   EPD; Q3V3E1; -.
DR   jPOST; Q3V3E1; -.
DR   MaxQB; Q3V3E1; -.
DR   PaxDb; Q3V3E1; -.
DR   PRIDE; Q3V3E1; -.
DR   ProteomicsDB; 297798; -.
DR   Antibodypedia; 23818; 334 antibodies from 35 providers.
DR   DNASU; 328795; -.
DR   Ensembl; ENSMUST00000236745; ENSMUSP00000158544; ENSMUSG00000042345.
DR   GeneID; 328795; -.
DR   KEGG; mmu:328795; -.
DR   UCSC; uc008bus.2; mouse.
DR   CTD; 53347; -.
DR   MGI; MGI:1926074; Ubash3a.
DR   VEuPathDB; HostDB:ENSMUSG00000042345; -.
DR   eggNOG; KOG3734; Eukaryota.
DR   GeneTree; ENSGT00940000160841; -.
DR   HOGENOM; CLU_016516_0_0_1; -.
DR   InParanoid; Q3V3E1; -.
DR   OMA; MCFCEEL; -.
DR   OrthoDB; 243749at2759; -.
DR   PhylomeDB; Q3V3E1; -.
DR   TreeFam; TF313334; -.
DR   BioGRID-ORCS; 328795; 2 hits in 73 CRISPR screens.
DR   ChiTaRS; Ubash3a; mouse.
DR   EvolutionaryTrace; Q3V3E1; -.
DR   PRO; PR:Q3V3E1; -.
DR   Proteomes; UP000000589; Chromosome 17.
DR   RNAct; Q3V3E1; protein.
DR   Bgee; ENSMUSG00000042345; Expressed in thymus and 56 other tissues.
DR   ExpressionAtlas; Q3V3E1; baseline and differential.
DR   Genevisible; Q3V3E1; MM.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR   GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR   GO; GO:0050860; P:negative regulation of T cell receptor signaling pathway; IGI:MGI.
DR   GO; GO:0001817; P:regulation of cytokine production; IGI:MGI.
DR   CDD; cd07067; HP_PGM_like; 1.
DR   CDD; cd11937; SH3_UBASH3A; 1.
DR   Gene3D; 3.40.50.1240; -; 1.
DR   InterPro; IPR013078; His_Pase_superF_clade-1.
DR   InterPro; IPR029033; His_PPase_superfam.
DR   InterPro; IPR036028; SH3-like_dom_sf.
DR   InterPro; IPR001452; SH3_domain.
DR   InterPro; IPR015940; UBA.
DR   InterPro; IPR009060; UBA-like_sf.
DR   InterPro; IPR035634; UBASH3A_SH3.
DR   Pfam; PF00300; His_Phos_1; 1.
DR   Pfam; PF14604; SH3_9; 1.
DR   Pfam; PF00627; UBA; 1.
DR   SMART; SM00326; SH3; 1.
DR   SUPFAM; SSF46934; SSF46934; 1.
DR   SUPFAM; SSF50044; SSF50044; 1.
DR   SUPFAM; SSF53254; SSF53254; 1.
DR   PROSITE; PS50002; SH3; 1.
DR   PROSITE; PS50030; UBA; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Nucleus; Reference proteome; SH3 domain.
FT   CHAIN           1..624
FT                   /note="Ubiquitin-associated and SH3 domain-containing
FT                   protein A"
FT                   /id="PRO_0000415372"
FT   DOMAIN          19..60
FT                   /note="UBA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00212"
FT   DOMAIN          238..303
FT                   /note="SH3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00192"
FT   REGION          358..624
FT                   /note="Phosphatase-like"
FT   CONFLICT        75
FT                   /note="A -> V (in Ref. 1; BAE41311)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        331
FT                   /note="C -> S (in Ref. 1; BAE41311)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        337
FT                   /note="P -> S (in Ref. 1; BAE41311)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        444
FT                   /note="S -> F (in Ref. 1; BAE41311)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        463
FT                   /note="E -> D (in Ref. 1; BAE41311)"
FT                   /evidence="ECO:0000305"
FT   STRAND          360..365
FT                   /evidence="ECO:0007829|PDB:3D4I"
FT   HELIX           370..374
FT                   /evidence="ECO:0007829|PDB:3D4I"
FT   HELIX           378..381
FT                   /evidence="ECO:0007829|PDB:3D4I"
FT   STRAND          385..387
FT                   /evidence="ECO:0007829|PDB:3DB1"
FT   HELIX           406..412
FT                   /evidence="ECO:0007829|PDB:3D4I"
FT   HELIX           418..434
FT                   /evidence="ECO:0007829|PDB:3D4I"
FT   STRAND          438..443
FT                   /evidence="ECO:0007829|PDB:3D4I"
FT   HELIX           447..459
FT                   /evidence="ECO:0007829|PDB:3D4I"
FT   TURN            463..465
FT                   /evidence="ECO:0007829|PDB:3D4I"
FT   STRAND          468..470
FT                   /evidence="ECO:0007829|PDB:3D4I"
FT   HELIX           472..474
FT                   /evidence="ECO:0007829|PDB:3D4I"
FT   HELIX           478..480
FT                   /evidence="ECO:0007829|PDB:3D4I"
FT   HELIX           485..488
FT                   /evidence="ECO:0007829|PDB:3D4I"
FT   HELIX           492..497
FT                   /evidence="ECO:0007829|PDB:3D4I"
FT   HELIX           512..514
FT                   /evidence="ECO:0007829|PDB:3D4I"
FT   HELIX           521..536
FT                   /evidence="ECO:0007829|PDB:3D4I"
FT   STRAND          544..550
FT                   /evidence="ECO:0007829|PDB:3D4I"
FT   HELIX           554..557
FT                   /evidence="ECO:0007829|PDB:3D4I"
FT   HELIX           560..563
FT                   /evidence="ECO:0007829|PDB:3D4I"
FT   HELIX           570..578
FT                   /evidence="ECO:0007829|PDB:3D4I"
FT   STRAND          585..590
FT                   /evidence="ECO:0007829|PDB:3D4I"
FT   TURN            592..594
FT                   /evidence="ECO:0007829|PDB:3D4I"
FT   STRAND          597..600
FT                   /evidence="ECO:0007829|PDB:3D4I"
FT   HELIX           617..620
FT                   /evidence="ECO:0007829|PDB:3D4I"
SQ   SEQUENCE   624 AA;  70145 MW;  E40AFB541AC84538 CRC64;
     MAAGETQLYA KVSNKLKGRS TPSLLDPLLA MGFPTHTALK ALAATGRKTA EAAADWLHGH
     CNDPSLDDPI PQEYALFLCP TGPLLEKLQE FWRESRRQCA KNRAHEVFPH VTLCDFFTCE
     DQKVECLYEA LRRAGDRILG SFPTLVPLVL HSSISYLGFF INDSPADAIR EFAMAFATEA
     AVLADCTIKP CTKQLHLTLA HKFYPHHQRT LEQLAKAIQP SHSCQWTAAL YSRDMRFVHY
     QTLKALFQYK PQNADELMLS AGDYIFVDPT QQEEASEGWA IGISHRTGCR GFLPENYTER
     ANEADTWVKH RTYTFNLAMD LNSRKDFEAS CRGNGEPHTP SMSKSVSSIQ ALQATISRRG
     ILVVRHGERV DQVFGKSWLQ QCTTADGKYY RPDLNFPRSL PRRSNGIKDF ENDPPLSSCG
     IFQARLAGEA LLDSGVRVTA VFASPALRCV QTAKHILEEL KLEKKLKIRV EPGIFEWMKW
     EASKATLTFL TLEELKEANF NVDLDYRPAL PRCSLMPAES YDQYVERCAV SMGQIINTCP
     QDMGITLIVS HSSALDSCTR PLLGLPPREC GDFAQLVRKI PSLGMCFCEE NREDGKWDLV
     NPPVKTLTHG ANSVFNWRNW ISSN
 
 
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