UBX10_HUMAN
ID UBX10_HUMAN Reviewed; 280 AA.
AC Q96LJ8; Q5R386;
DT 23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=UBX domain-containing protein 10 {ECO:0000305};
DE AltName: Full=UBX domain-containing protein 3;
GN Name=UBXN10 {ECO:0000312|HGNC:HGNC:26354};
GN Synonyms=UBXD3 {ECO:0000312|HGNC:HGNC:26354};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CLUAP1 AND VCP, AND
RP MUTAGENESIS OF MET-244; GLU-245; VAL-246; PRO-247 AND ARG-248.
RX PubMed=26389662; DOI=10.1038/ncb3238;
RA Raman M., Sergeev M., Garnaas M., Lydeard J.R., Huttlin E.L., Goessling W.,
RA Shah J.V., Harper J.W.;
RT "Systematic proteomics of the VCP-UBXD adaptor network identifies a role
RT for UBXN10 in regulating ciliogenesis.";
RL Nat. Cell Biol. 17:1356-1369(2015).
CC -!- FUNCTION: VCP/p97-binding protein required for ciliogenesis
CC (PubMed:26389662). Acts as a tethering factor that facilitates
CC recruitment of VCP/p97 to the intraflagellar transport complex B (IFT-
CC B) in cilia (PubMed:26389662). UBX domain-containing proteins act as
CC tethering factors for VCP/p97 and may specify substrate specificity of
CC VCP/p97 (PubMed:26389662). {ECO:0000269|PubMed:26389662}.
CC -!- SUBUNIT: Interacts with CLUAP1; the interaction is direct and mediates
CC interaction with the intraflagellar transport complex B (IFT-B)
CC (PubMed:26389662). Interacts with VCP; the interaction is direct
CC (PubMed:26389662). {ECO:0000269|PubMed:26389662}.
CC -!- INTERACTION:
CC Q96LJ8; Q9BZV1: UBXN6; NbExp=3; IntAct=EBI-1993941, EBI-1993899;
CC Q96LJ8; P55072: VCP; NbExp=7; IntAct=EBI-1993941, EBI-355164;
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium
CC {ECO:0000269|PubMed:26389662}. Note=Recruited to cilia in a VCP-
CC dependent manner. {ECO:0000269|PubMed:26389662}.
CC -!- SIMILARITY: Belongs to the UBXN10 family. {ECO:0000305}.
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DR EMBL; AK058158; BAB71693.1; -; mRNA.
DR EMBL; Z98257; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471134; EAW94920.1; -; Genomic_DNA.
DR EMBL; BC047428; AAH47428.1; -; mRNA.
DR CCDS; CCDS205.1; -.
DR RefSeq; NP_689589.1; NM_152376.4.
DR RefSeq; XP_005245799.1; XM_005245742.3.
DR RefSeq; XP_011539001.1; XM_011540699.2.
DR AlphaFoldDB; Q96LJ8; -.
DR SMR; Q96LJ8; -.
DR BioGRID; 126082; 40.
DR IntAct; Q96LJ8; 7.
DR MINT; Q96LJ8; -.
DR STRING; 9606.ENSP00000364240; -.
DR iPTMnet; Q96LJ8; -.
DR PhosphoSitePlus; Q96LJ8; -.
DR BioMuta; UBXN10; -.
DR DMDM; 55976757; -.
DR jPOST; Q96LJ8; -.
DR MassIVE; Q96LJ8; -.
DR PaxDb; Q96LJ8; -.
DR PeptideAtlas; Q96LJ8; -.
DR PRIDE; Q96LJ8; -.
DR ProteomicsDB; 77215; -.
DR Antibodypedia; 29784; 181 antibodies from 22 providers.
DR DNASU; 127733; -.
DR Ensembl; ENST00000375099.4; ENSP00000364240.3; ENSG00000162543.6.
DR GeneID; 127733; -.
DR KEGG; hsa:127733; -.
DR MANE-Select; ENST00000375099.4; ENSP00000364240.3; NM_152376.5; NP_689589.1.
DR UCSC; uc001bdb.4; human.
DR CTD; 127733; -.
DR GeneCards; UBXN10; -.
DR HGNC; HGNC:26354; UBXN10.
DR HPA; ENSG00000162543; Tissue enhanced (fallopian).
DR neXtProt; NX_Q96LJ8; -.
DR OpenTargets; ENSG00000162543; -.
DR PharmGKB; PA162408337; -.
DR VEuPathDB; HostDB:ENSG00000162543; -.
DR eggNOG; ENOG502S4IN; Eukaryota.
DR GeneTree; ENSGT00390000012939; -.
DR HOGENOM; CLU_079919_0_0_1; -.
DR InParanoid; Q96LJ8; -.
DR OMA; ECRIPHK; -.
DR OrthoDB; 1474017at2759; -.
DR PhylomeDB; Q96LJ8; -.
DR TreeFam; TF335927; -.
DR PathwayCommons; Q96LJ8; -.
DR SignaLink; Q96LJ8; -.
DR BioGRID-ORCS; 127733; 6 hits in 1071 CRISPR screens.
DR ChiTaRS; UBXN10; human.
DR GenomeRNAi; 127733; -.
DR Pharos; Q96LJ8; Tdark.
DR PRO; PR:Q96LJ8; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q96LJ8; protein.
DR Bgee; ENSG00000162543; Expressed in bronchial epithelial cell and 155 other tissues.
DR Genevisible; Q96LJ8; HS.
DR GO; GO:0005929; C:cilium; IDA:UniProtKB.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0043130; F:ubiquitin binding; IBA:GO_Central.
DR GO; GO:0060271; P:cilium assembly; IDA:UniProtKB.
DR GO; GO:0030433; P:ubiquitin-dependent ERAD pathway; IBA:GO_Central.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR InterPro; IPR001012; UBX_dom.
DR Pfam; PF00789; UBX; 1.
DR SMART; SM00166; UBX; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS50033; UBX; 1.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Cilium biogenesis/degradation; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..280
FT /note="UBX domain-containing protein 10"
FT /id="PRO_0000211029"
FT DOMAIN 194..271
FT /note="UBX"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00215"
FT REGION 41..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 87
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MUTAGEN 244
FT /note="M->A: Reduced interaction with VCP."
FT /evidence="ECO:0000269|PubMed:26389662"
FT MUTAGEN 245
FT /note="E->A: Reduced interaction with VCP."
FT /evidence="ECO:0000269|PubMed:26389662"
FT MUTAGEN 246
FT /note="V->A: Reduced interaction with VCP."
FT /evidence="ECO:0000269|PubMed:26389662"
FT MUTAGEN 247
FT /note="P->A: Reduced interaction with VCP."
FT /evidence="ECO:0000269|PubMed:26389662"
FT MUTAGEN 248
FT /note="R->A: Reduced interaction with VCP."
FT /evidence="ECO:0000269|PubMed:26389662"
SQ SEQUENCE 280 AA; 30811 MW; A0B5D24C7F01F947 CRC64;
MATEAPVNIA PPECSTVVST AVDSLIWQPN SLNMHMIRPK SAKGRTRPSL QKSQGVEVCA
HHIPSPPPAI PYELPSSQKP GACAPKSPNQ GASDEIPELQ QQVPTGASSS LNKYPVLPSI
NRKNLEEEAV ETVAKKASSL QLSSIRALYQ DETGTMKTSE EDSRARACAV ERKFIVRTKK
QGSSRAGNLE EPSDQEPRLL LAVRSPTGQR FVRHFRPTDD LQTIVAVAEQ KNKTSYRHCS
IETMEVPRRR FSDLTKSLQE CRIPHKSVLG ISLEDGEGWP
