UCC1_ARATH
ID UCC1_ARATH Reviewed; 261 AA.
AC O82081; Q0WPC5;
DT 05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Uclacyanin 1 {ECO:0000303|PubMed:9761472};
DE Flags: Precursor;
GN Name=UCC1 {ECO:0000303|PubMed:9761472};
GN OrderedLocusNames=At2g32300 {ECO:0000312|Araport:AT2G32300};
GN ORFNames=T32F6 {ECO:0000305};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GLYCOSYLATION, BIOPHYSICOCHEMICAL PROPERTIES,
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=9761472; DOI=10.1002/pro.5560070907;
RA Nersissian A.M., Immoos C., Hill M.G., Hart P.J., Williams G.,
RA Herrmann R.G., Valentine J.S.;
RT "Uclacyanins, stellacyanins, and plantacyanins are distinct subfamilies of
RT phytocyanins: plant-specific mononuclear blue copper proteins.";
RL Protein Sci. 7:1915-1929(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY.
RX PubMed=17293437; DOI=10.1104/pp.106.092296;
RA Khan J.A., Wang Q., Sjoelund R.D., Schulz A., Thompson G.A.;
RT "An early nodulin-like protein accumulates in the sieve element plasma
RT membrane of Arabidopsis.";
RL Plant Physiol. 143:1576-1589(2007).
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Absorption:
CC Abs(max)=~600 nm {ECO:0000269|PubMed:9761472};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255}; Lipid-anchor, GPI-
CC anchor {ECO:0000255}.
CC -!- PTM: Glycosylated. {ECO:0000303|PubMed:9761472}.
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DR EMBL; U76298; AAC32038.1; -; mRNA.
DR EMBL; AC005700; AAC69948.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08665.1; -; Genomic_DNA.
DR EMBL; AK229150; BAF01024.1; -; mRNA.
DR PIR; D84731; D84731.
DR RefSeq; NP_180789.1; NM_128789.3.
DR AlphaFoldDB; O82081; -.
DR SMR; O82081; -.
DR STRING; 3702.AT2G32300.1; -.
DR MetOSite; O82081; -.
DR PaxDb; O82081; -.
DR ProteomicsDB; 243241; -.
DR EnsemblPlants; AT2G32300.1; AT2G32300.1; AT2G32300.
DR GeneID; 817791; -.
DR Gramene; AT2G32300.1; AT2G32300.1; AT2G32300.
DR KEGG; ath:AT2G32300; -.
DR Araport; AT2G32300; -.
DR TAIR; locus:2062525; AT2G32300.
DR eggNOG; ENOG502RZRN; Eukaryota.
DR HOGENOM; CLU_058719_2_1_1; -.
DR InParanoid; O82081; -.
DR PRO; PR:O82081; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O82081; baseline and differential.
DR GO; GO:0031225; C:anchored component of membrane; TAS:TAIR.
DR GO; GO:0046658; C:anchored component of plasma membrane; IBA:GO_Central.
DR GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 2.60.40.420; -; 1.
DR InterPro; IPR028871; BlueCu_1_BS.
DR InterPro; IPR008972; Cupredoxin.
DR InterPro; IPR039391; Phytocyanin.
DR InterPro; IPR003245; Phytocyanin_dom.
DR PANTHER; PTHR33021; PTHR33021; 1.
DR Pfam; PF02298; Cu_bind_like; 1.
DR SUPFAM; SSF49503; SSF49503; 1.
DR PROSITE; PS00196; COPPER_BLUE; 1.
DR PROSITE; PS51485; PHYTOCYANIN; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Copper; Disulfide bond; Glycoprotein; GPI-anchor;
KW Lipoprotein; Membrane; Metal-binding; Reference proteome; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..237
FT /note="Uclacyanin 1"
FT /evidence="ECO:0000255"
FT /id="PRO_5006739294"
FT PROPEP 238..261
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000437143"
FT DOMAIN 24..123
FT /note="Phytocyanin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00818"
FT REGION 194..239
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 199..229
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 64
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00818"
FT BINDING 105
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00818"
FT BINDING 110
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00818"
FT BINDING 115
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00818"
FT LIPID 237
FT /note="GPI-anchor amidated alanine"
FT /evidence="ECO:0000255"
FT DISULFID 77..111
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00818"
FT CONFLICT 226
FT /note="T -> M (in Ref. 4; BAF01024)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 261 AA; 26451 MW; 8C2BB83F749A764E CRC64;
MASREMLIII SVLATTLIGL TVATDHTIGG PSGWTVGASL RTWAAGQTFA VGDNLVFSYP
AAFHDVVEVT KPEFDSCQAV KPLITFANGN SLVPLTTPGK RYFICGMPGH CSQGMKLEVN
VVPTATVAPT APLPNTVPSL NAPSPSSVLP IQPLLPLNPV PVLSPSSSTP LPSSSLPLIP
PLSPALSPAT AAGTSLPLFP GSPGSSSSTT STKTVGTFPS STTGTTADLA GADSPPADSS
SAAKTLVLGF GFMVAMMLHL F
