ID   UCHL_APLCA              Reviewed;         214 AA.
AC   O01391;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Ubiquitin carboxyl-terminal hydrolase;
DE            EC=3.4.19.12;
DE   AltName: Full=Ubiquitin thioesterase;
GN   Name=UCH;
OS   Aplysia californica (California sea hare).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Heterobranchia; Euthyneura; Tectipleura; Aplysiida; Aplysioidea;
OC   Aplysiidae; Aplysia.
OX   NCBI_TaxID=6500;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=9094720; DOI=10.1016/s0092-8674(00)80188-9;
RA   Hegde A.N., Inokuchi K., Pei W., Casadio A., Ghirardi M., Chain D.G.,
RA   Martin K.C., Kandel E.R., Schwartz J.H.;
RT   "Ubiquitin C-terminal hydrolase is an immediate-early gene essential for
RT   long-term facilitation in Aplysia.";
RL   Cell 89:115-126(1997).
CC   -!- FUNCTION: Ubiquitin-protein hydrolase is involved both in the
CC       processing of ubiquitin precursors and of ubiquitinated proteins. This
CC       enzyme is a thiol protease that recognizes and hydrolyzes a peptide
CC       bond at the C-terminal glycine of ubiquitin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide
CC         and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-
CC         residue protein attached to proteins as an intracellular targeting
CC         signal).; EC=3.4.19.12;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the peptidase C12 family. {ECO:0000305}.
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DR   EMBL; U90177; AAB52410.1; -; mRNA.
DR   RefSeq; NP_001191493.1; NM_001204564.1.
DR   AlphaFoldDB; O01391; -.
DR   SMR; O01391; -.
DR   MEROPS; C12.003; -.
DR   GeneID; 100533253; -.
DR   CTD; 100533253; -.
DR   OrthoDB; 1013351at2759; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004843; F:cysteine-type deubiquitinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro.
DR   Gene3D; 3.40.532.10; -; 1.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   InterPro; IPR001578; Peptidase_C12_UCH.
DR   InterPro; IPR036959; Peptidase_C12_UCH_sf.
DR   PANTHER; PTHR10589; PTHR10589; 1.
DR   Pfam; PF01088; Peptidase_C12; 1.
DR   PRINTS; PR00707; UBCTHYDRLASE.
DR   SUPFAM; SSF54001; SSF54001; 1.
DR   PROSITE; PS00140; UCH_1; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Hydrolase; Protease; Thiol protease; Ubl conjugation pathway.
FT   CHAIN           1..214
FT                   /note="Ubiquitin carboxyl-terminal hydrolase"
FT                   /id="PRO_0000211068"
FT   ACT_SITE        90
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10091"
FT   ACT_SITE        164
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10091"
FT   SITE            179
FT                   /note="Important for enzyme activity"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   214 AA;  23703 MW;  07ACDEDAADB05D6E CRC64;
     MASEQRWIPL ESNPKVLNKY VHNLGMDAGW NFVDVFGLDP ELLAMVPRPA AALVLLFPDD
     KETVNQLIGE YQSDYPDSLY YTKQTIGNAC GTVAIVHALA NNENVIPFDA AKHFKTFLEK
     TKPLNPEERA KHLEQDNLMG AAHGDCAQEG DTQAPSQDEH VKSHFVALVH CNGTLYELDG
     RKEAPVVHGT TSADTFLEDA AEVVKKFMAR DPEI