UCP4C_DROME
ID UCP4C_DROME Reviewed; 335 AA.
AC Q9VMK1;
DT 18-JAN-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Mitochondrial uncoupling protein 4C {ECO:0000303|PubMed:24639557};
GN Name=Ucp4C {ECO:0000303|PubMed:24639557, ECO:0000312|FlyBase:FBgn0031757};
GN ORFNames=CG9064 {ECO:0000312|FlyBase:FBgn0031757};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAL90083.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAL90083.1};
RC TISSUE=Testis {ECO:0000312|EMBL:AAL90083.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=24639557; DOI=10.1002/embr.201337972;
RA Da-Re C., De Pitta C., Zordan M.A., Teza G., Nestola F., Zeviani M.,
RA Costa R., Bernardi P.;
RT "UCP4C mediates uncoupled respiration in larvae of Drosophila
RT melanogaster.";
RL EMBO Rep. 15:586-591(2014).
CC -!- FUNCTION: Mitochondrial protein that is likely to be responsible for
CC thermogenic respiration. Likely to function in mitochondrial uncoupling
CC i.e. creating mitochondrial proton leaks across the inner mitochondrial
CC membrane and can therefore dissipate the mitochondrial proton gradient
CC and convert the energy of substrate oxidation into heat instead of ATP.
CC Involved in cold tolerance, it is required for development to the adult
CC stage at low temperatures. {ECO:0000269|PubMed:24639557}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown is adult lethal at low
CC temperatures. At 15 degrees Celsius there is a severe reduction in the
CC number of larvae and flies do not survive to the adult stage. Second
CC instar larvae moved from 23 to 14 degrees Celsius show no heat
CC production in response to the decrease in temperature. In second instar
CC larvae body wall preparations, maximal oxygen consumption rate (i.e.
CC mitochondrial respiration) is significantly higher than the controls,
CC and the addition of the uncoupler carbonyl cyanide p-
CC [trifluoromethoxy]-phenyl-hydrazone followed by the ATP inhibitor
CC oligomycin results in a threefold increase in the oxygen consumption
CC rate. Uncoupling in larval mitochondria in response to the addition of
CC palmitate is also suppressed. {ECO:0000269|PubMed:24639557}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
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DR EMBL; AE014134; AAF52313.1; -; Genomic_DNA.
DR EMBL; AY089345; AAL90083.1; -; mRNA.
DR RefSeq; NP_608976.1; NM_135132.3.
DR AlphaFoldDB; Q9VMK1; -.
DR STRING; 7227.FBpp0078803; -.
DR PaxDb; Q9VMK1; -.
DR PRIDE; Q9VMK1; -.
DR DNASU; 33832; -.
DR EnsemblMetazoa; FBtr0079172; FBpp0078803; FBgn0031757.
DR GeneID; 33832; -.
DR KEGG; dme:Dmel_CG9064; -.
DR UCSC; CG9064-RA; d. melanogaster.
DR CTD; 33832; -.
DR FlyBase; FBgn0031757; Ucp4C.
DR VEuPathDB; VectorBase:FBgn0031757; -.
DR eggNOG; KOG0753; Eukaryota.
DR GeneTree; ENSGT00940000160098; -.
DR HOGENOM; CLU_015166_14_2_1; -.
DR InParanoid; Q9VMK1; -.
DR OMA; RACLMTA; -.
DR OrthoDB; 1126848at2759; -.
DR PhylomeDB; Q9VMK1; -.
DR BioGRID-ORCS; 33832; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 33832; -.
DR PRO; PR:Q9VMK1; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0031757; Expressed in testis and 9 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0009409; P:response to cold; IBA:GO_Central.
DR Gene3D; 1.50.40.10; -; 1.
DR InterPro; IPR002067; Mit_carrier.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR Pfam; PF00153; Mito_carr; 3.
DR PRINTS; PR00926; MITOCARRIER.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 2: Evidence at transcript level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Repeat; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..335
FT /note="Mitochondrial uncoupling protein 4C"
FT /id="PRO_0000438777"
FT TRANSMEM 40..57
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..118
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..157
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..223
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..264
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 304..323
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REPEAT 34..125
FT /note="Solcar 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT REPEAT 137..229
FT /note="Solcar 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT REPEAT 238..329
FT /note="Solcar 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
SQ SEQUENCE 335 AA; 38194 MW; 4750453F0828B4DE CRC64;
MDKAERDYWH LRSLEIEEEP RFPPTNVADP LTARNLFQLY VNTFIGANLA ESCVFPLDVA
KTRMQVDGEQ AKKTGKAMPT FRATLTNMIR VEGFKSLYAG FSAMVTRNFI FNSLRVVLYD
VFRRPFLYQN ERNEEVLKIY MALGCSFTAG CIAQALANPF DIVKVRMQTE GRRRQLGYDV
RVNSMVQAFV DIYRRGGLPS MWKGVGPSCM RACLMTTGDV GSYDISKRTF KRLLDLEEGL
PLRFVSSMCA GLTASVLSTP ADVIKSRMMN QPVDESGKNL YYKNSLDCVR KLVREEGVLT
LYKGLMPTWF RLGPFSVLFW LSVEQLRQWE GQSGF
