UCP8_SCHPO
ID UCP8_SCHPO Reviewed; 829 AA.
AC O94685;
DT 29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=UBA domain-containing protein 8;
GN Name=ucp8; ORFNames=SPBC83.01;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP REVISION OF GENE MODEL.
RX PubMed=21511999; DOI=10.1126/science.1203357;
RA Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT "Comparative functional genomics of the fission yeasts.";
RL Science 332:930-936(2011).
RN [3]
RP IDENTIFICATION.
RX PubMed=11584278; DOI=10.1038/ncb1001-939;
RA Wilkinson C.R.M., Seeger M., Hartmann-Petersen R., Stone M., Wallace M.,
RA Semple C., Gordon C.;
RT "Proteins containing the UBA domain are able to bind to multi-ubiquitin
RT chains.";
RL Nat. Cell Biol. 3:939-943(2001).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-112 AND SER-113, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
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DR EMBL; CU329671; CAB36863.2; -; Genomic_DNA.
DR PIR; T40690; T40690.
DR RefSeq; NP_595633.2; NM_001021527.2.
DR AlphaFoldDB; O94685; -.
DR STRING; 4896.SPBC83.01.1; -.
DR iPTMnet; O94685; -.
DR SwissPalm; O94685; -.
DR MaxQB; O94685; -.
DR PaxDb; O94685; -.
DR PRIDE; O94685; -.
DR EnsemblFungi; SPBC83.01.1; SPBC83.01.1:pep; SPBC83.01.
DR GeneID; 2540786; -.
DR KEGG; spo:SPBC83.01; -.
DR PomBase; SPBC83.01; ucp8.
DR VEuPathDB; FungiDB:SPBC83.01; -.
DR eggNOG; KOG0998; Eukaryota.
DR HOGENOM; CLU_341989_0_0_1; -.
DR InParanoid; O94685; -.
DR OMA; RIEMSVA; -.
DR Reactome; R-SPO-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR Reactome; R-SPO-8856828; Clathrin-mediated endocytosis.
DR Reactome; R-SPO-9013420; RHOU GTPase cycle.
DR PRO; PR:O94685; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0030479; C:actin cortical patch; ISO:PomBase.
DR GO; GO:0005509; F:calcium ion binding; ISM:PomBase.
DR GO; GO:0031593; F:polyubiquitin modification-dependent protein binding; TAS:PomBase.
DR GO; GO:0043130; F:ubiquitin binding; ISM:PomBase.
DR GO; GO:0000147; P:actin cortical patch assembly; ISO:PomBase.
DR GO; GO:0006897; P:endocytosis; ISS:PomBase.
DR GO; GO:0016197; P:endosomal transport; IBA:GO_Central.
DR GO; GO:0006886; P:intracellular protein transport; IC:PomBase.
DR CDD; cd00052; EH; 3.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR000261; EH_dom.
DR InterPro; IPR015940; UBA.
DR InterPro; IPR009060; UBA-like_sf.
DR Pfam; PF12763; EF-hand_4; 2.
DR Pfam; PF00627; UBA; 1.
DR SMART; SM00027; EH; 3.
DR SMART; SM00165; UBA; 1.
DR SUPFAM; SSF46934; SSF46934; 1.
DR SUPFAM; SSF47473; SSF47473; 3.
DR PROSITE; PS50222; EF_HAND_2; 2.
DR PROSITE; PS50031; EH; 3.
DR PROSITE; PS50030; UBA; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..829
FT /note="UBA domain-containing protein 8"
FT /id="PRO_0000065716"
FT DOMAIN 10..109
FT /note="EH 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00077"
FT DOMAIN 43..78
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 129..225
FT /note="EH 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00077"
FT DOMAIN 300..398
FT /note="EH 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00077"
FT DOMAIN 333..368
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 788..828
FT /note="UBA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00212"
FT REGION 93..121
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 466..520
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 574..614
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 724..785
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 602..709
FT /evidence="ECO:0000255"
FT COMPBIAS 98..113
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 466..502
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 725..755
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 756..785
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 112
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 113
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
SQ SEQUENCE 829 AA; 91119 MW; EA6D9B346BA9A60F CRC64;
MQPLQLSPIE QQEFDRLLES LHPEQKDLIP GSVIGPFLLK FGLPQKILAK IWDYCDQEDK
GSLDRNQVYA CFRLISQAQR GATLQELDYN KAGDPPILPK QAKDDHHIKR SSSETADFTP
FRIPINPDER SEYEKEFREN LRGHEDSARP GCMPSSIAKP ILLKSSLHYA VLAQIWNLAD
SAHVGYLEMY QYVIARHLVA ICKQYNLIHL PRSLPADVIE SAKSETPAMN TNIEQGVTQP
SLVNTEQLSH QQSISSTKSS KLDEISADNN AQSAVDNKYS SPPQTIDPQA NIKALITPED
RSNFYQLFSK IDNENKGYIV GGEAVPFFMA SHLDSEELAR IWDTVDTQDR GYIDKDEFAV
AMEIIKLRLS GKSLASILAY DDNPTEPTMQ QNLVSDDTNA STAVPVAFTN DNVSNVPLSV
ADNEPNSTLN LLASSDTAAF EPTAVSLHEN PSSSLEDLQS AFQNTSFQDQ VSTQNQANTA
DISQNTESGS STQGQMFGIP PTTQSIPLKM AGFPGGVNSS LTDIKEEEAV SENAPQQEER
SFEQIKTSIH IAPENISAIE ESKSVPLPTS FATTIPGSTS AALDDQQTTE APFEEPDEPA
HEPTEEEQEE MRKLEEKIES TKYGLETIQT SGKTIKQRIE QKKTRLMILR EELQELDAIK
EEAQKQISQS LIADQQLSTQ IGSVGMGKKQ AVKELRKLQA KIDSIIADSM SPMGTGAVNS
PAIKPQVTPA PPTPAPTPAV KHHPPPPPVR SSISPSMPPA PLTHANSSTP MNYVSQPESP
PQSYESIQND NELLQELLSM GFPREKAVIA LEATNYDVNE AANILLSSV
