UCPA_ECOLI
ID UCPA_ECOLI Reviewed; 263 AA.
AC P37440; P76963; P77140; P77442;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2001, sequence version 3.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Oxidoreductase UcpA;
DE EC=1.-.-.-;
GN Name=ucpA; Synonyms=yfeF; OrderedLocusNames=b2426, JW5394;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=9241368;
RA Sirko A., Weglenska A., Hryniewicz M.M., Hulanicka D.M.;
RT "Characterization of the Escherichia coli gene encoding a new member of the
RT short-chain dehydrogenase/reductase (SDR) family.";
RL Acta Biochim. Pol. 44:153-157(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 180-263.
RC STRAIN=K12;
RX PubMed=2188959; DOI=10.1128/jb.172.6.3358-3366.1990;
RA Hryniewicz M.M., Sirko A., Palucha A., Boeck A., Hulanicka D.M.;
RT "Sulfate and thiosulfate transport in Escherichia coli K-12: identification
RT of a gene encoding a novel protein involved in thiosulfate binding.";
RL J. Bacteriol. 172:3358-3366(1990).
RN [6]
RP IDENTIFICATION.
RX PubMed=7984428; DOI=10.1093/nar/22.22.4756;
RA Borodovsky M., Rudd K.E., Koonin E.V.;
RT "Intrinsic and extrinsic approaches for detecting genes in a bacterial
RT genome.";
RL Nucleic Acids Res. 22:4756-4767(1994).
CC -!- INTERACTION:
CC P37440; P0A796: pfkA; NbExp=2; IntAct=EBI-559387, EBI-554405;
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
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DR EMBL; X99908; CAA68181.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75479.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA16309.2; -; Genomic_DNA.
DR EMBL; M32101; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; A65017; A65017.
DR RefSeq; NP_416921.4; NC_000913.3.
DR RefSeq; WP_000517431.1; NZ_STEB01000039.1.
DR AlphaFoldDB; P37440; -.
DR SMR; P37440; -.
DR BioGRID; 4260683; 11.
DR DIP; DIP-11073N; -.
DR IntAct; P37440; 3.
DR STRING; 511145.b2426; -.
DR jPOST; P37440; -.
DR PaxDb; P37440; -.
DR PRIDE; P37440; -.
DR EnsemblBacteria; AAC75479; AAC75479; b2426.
DR EnsemblBacteria; BAA16309; BAA16309; BAA16309.
DR GeneID; 66673704; -.
DR GeneID; 946898; -.
DR KEGG; ecj:JW5394; -.
DR KEGG; eco:b2426; -.
DR PATRIC; fig|1411691.4.peg.4305; -.
DR EchoBASE; EB2054; -.
DR eggNOG; COG1028; Bacteria.
DR HOGENOM; CLU_010194_1_0_6; -.
DR InParanoid; P37440; -.
DR OMA; GALPHMY; -.
DR PhylomeDB; P37440; -.
DR BioCyc; EcoCyc:EG12133-MON; -.
DR BioCyc; MetaCyc:EG12133-MON; -.
DR PRO; PR:P37440; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0052588; F:diacetyl reductase ((S)-acetoin forming) activity; IDA:EcoCyc.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR002347; SDR_fam.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 1: Evidence at protein level;
KW Oxidoreductase; Reference proteome.
FT CHAIN 1..263
FT /note="Oxidoreductase UcpA"
FT /id="PRO_0000054801"
FT ACT_SITE 155
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 10..32
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 141
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT CONFLICT 153
FT /note="T -> Q (in Ref. 1; CAA68181)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 263 AA; 27850 MW; 2E201713357FDF41 CRC64;
MGKLTGKTAL ITGALQGIGE GIARTFARHG ANLILLDISP EIEKLADELC GRGHRCTAVV
ADVRDPASVA AAIKRAKEKE GRIDILVNNA GVCRLGSFLD MSDDDRDFHI DINIKGVWNV
TKAVLPEMIA RKDGRIVMMS SVTGDMVADP GETAYALTKA AIVGLTKSLA VEYAQSGIRV
NAICPGYVRT PMAESIARQS NPEDPESVLT EMAKAIPMRR LADPLEVGEL AAFLASDESS
YLTGTQNVID GGSTLPETVS VGI
