UCRIA_CYAPA
ID UCRIA_CYAPA Reviewed; 239 AA.
AC Q5CC93;
DT 05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 12-APR-2005, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=Cytochrome b6-f complex iron-sulfur subunit 1, cyanelle;
DE EC=7.1.1.6;
DE AltName: Full=Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein 1;
DE AltName: Full=Rieske iron-sulfur protein 1;
DE Short=ISP 1;
DE Short=RISP 1;
DE Flags: Precursor;
GN Name=petC-1;
OS Cyanophora paradoxa.
OC Eukaryota; Glaucocystophyceae; Cyanophoraceae; Cyanophora.
OX NCBI_TaxID=2762;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN IMPORT INTO ISOLATED CYANELLES.
RC STRAIN=UTEX LB 555 / Pringsheim;
RX PubMed=15691332; DOI=10.1111/j.1742-4658.2004.04533.x;
RA Steiner J.M., Berghoefer J., Yusa F., Pompe J.A., Kloesgen R.B.,
RA Loeffelhardt W.;
RT "Conservative sorting in a primitive plastid. The cyanelle of Cyanophora
RT paradoxa.";
RL FEBS J. 272:987-998(2005).
CC -!- FUNCTION: Component of the cytochrome b6-f complex, which mediates
CC electron transfer between photosystem II (PSII) and photosystem I
CC (PSI), cyclic electron flow around PSI, and state transitions.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a plastoquinol + 2 H(+)(in) + 2 oxidized [plastocyanin] = a
CC plastoquinone + 4 H(+)(out) + 2 reduced [plastocyanin];
CC Xref=Rhea:RHEA:22148, Rhea:RHEA-COMP:9561, Rhea:RHEA-COMP:9562,
CC Rhea:RHEA-COMP:10039, Rhea:RHEA-COMP:10040, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17757, ChEBI:CHEBI:29036, ChEBI:CHEBI:49552,
CC ChEBI:CHEBI:62192; EC=7.1.1.6;
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135; Evidence={ECO:0000250};
CC Note=Binds 1 [2Fe-2S] cluster per subunit. {ECO:0000250};
CC -!- SUBUNIT: The 4 large subunits of the cytochrome b6-f complex are
CC cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and
CC the Rieske protein, while the 4 small subunits are petG, petL, petM and
CC petN. The complex functions as a dimer (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, cyanelle thylakoid membrane
CC {ECO:0000250}; Single-pass membrane protein {ECO:0000250}. Note=The
CC transmembrane helix obliquely spans the membrane in one monomer, and
CC its extrinsic C-terminal domain is part of the other monomer.
CC {ECO:0000250}.
CC -!- MISCELLANEOUS: This protein is 1 of 2 subunits of the cytochrome b6-f
CC complex that are encoded in the nucleus.
CC -!- MISCELLANEOUS: The Rieske iron-sulfur protein is a high potential 2Fe-
CC 2S protein.
CC -!- SIMILARITY: Belongs to the Rieske iron-sulfur protein family.
CC {ECO:0000255|HAMAP-Rule:MF_01335}.
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DR EMBL; AJ784852; CAH04960.1; -; mRNA.
DR AlphaFoldDB; Q5CC93; -.
DR SMR; Q5CC93; -.
DR GO; GO:0033115; C:cyanelle thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0045158; F:electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009496; F:plastoquinol--plastocyanin reductase activity; IEA:UniProtKB-EC.
DR Gene3D; 2.102.10.10; -; 1.
DR HAMAP; MF_01335; Cytb6_f_Rieske; 1.
DR InterPro; IPR023960; Cyt_b6_f_Rieske.
DR InterPro; IPR017941; Rieske_2Fe-2S.
DR InterPro; IPR036922; Rieske_2Fe-2S_sf.
DR InterPro; IPR014349; Rieske_Fe-S_prot.
DR InterPro; IPR005805; Rieske_Fe-S_prot_C.
DR PANTHER; PTHR10134; PTHR10134; 1.
DR Pfam; PF00355; Rieske; 1.
DR PRINTS; PR00162; RIESKE.
DR SUPFAM; SSF50022; SSF50022; 1.
DR PROSITE; PS51296; RIESKE; 1.
PE 2: Evidence at transcript level;
KW 2Fe-2S; Cyanelle; Disulfide bond; Electron transport; Iron; Iron-sulfur;
KW Membrane; Metal-binding; Plastid; Thylakoid; Transit peptide; Translocase;
KW Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..60
FT /note="Cyanelle"
FT /evidence="ECO:0000255"
FT CHAIN 61..239
FT /note="Cytochrome b6-f complex iron-sulfur subunit 1,
FT cyanelle"
FT /id="PRO_0000030687"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 125..221
FT /note="Rieske"
FT BINDING 167
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000250"
FT BINDING 169
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000250"
FT BINDING 185
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000250"
FT BINDING 188
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000250"
FT DISULFID 172..187
FT /evidence="ECO:0000250"
SQ SEQUENCE 239 AA; 24735 MW; 0D95E0A5458109CB CRC64;
MAFTTTAVVA PRGAKITGQS STCAIQNGKT VAVGTSKQVG SFKPVFAAAK PAKETTFSVS
CSAASDDVPD MGKRKLMNLL LLGAIAGPVA GAGGPFVSFL VPPKAGGGAG AGQAAKDALG
NDVKVSSWLE THKPGDRSLA QGLKGDATYL IVKEDGTLEN YGLNAVCTHL GCVVPWNASE
NKFMCPCHGS QYDRTGKVVR GPAPLSLALA HVSVLEDGVV AFEPWTETDF RTNTAPWWK
