UCRIA_SPIOL
ID UCRIA_SPIOL Reviewed; 230 AA.
AC P08980;
DT 01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT 21-JUN-2005, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Cytochrome b6-f complex iron-sulfur subunit, chloroplastic;
DE EC=7.1.1.6;
DE AltName: Full=Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein;
DE AltName: Full=Rieske iron-sulfur protein;
DE Short=ISP;
DE Short=RISP;
DE Flags: Precursor;
GN Name=petC;
OS Spinacia oleracea (Spinach).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia.
OX NCBI_TaxID=3562;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Monatol;
RA Steppuhn J., Rother C., Hermans J., Jansen T., Salnikow J., Hauska G.,
RA Herrmann R.G.;
RT "The complete amino-acid sequence of the Rieske FeS-precursor protein from
RT spinach chloroplasts deduced from cDNA analysis.";
RL Mol. Gen. Genet. 210:171-177(1987).
RN [2]
RP PROTEIN SEQUENCE OF 52-147.
RA Pfefferkorn B., Meyer H.E.;
RT "N-terminal amino acid sequence of the Rieske iron-sulfur protein from the
RT cytochrome b6/f-complex of spinach thylakoids.";
RL FEBS Lett. 206:233-237(1986).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (1.83 ANGSTROMS) OF 92-230 IN COMPLEX WITH 2FE-2S
RP CLUSTER, AND COFACTOR.
RX PubMed=9438861; DOI=10.1016/s0969-2126(97)00309-2;
RA Carrell C.J., Zhang H., Cramer W.A., Smith J.L.;
RT "Biological identity and diversity in photosynthesis and respiration:
RT structure of the lumen-side domain of the chloroplast Rieske protein.";
RL Structure 5:1613-1625(1997).
CC -!- FUNCTION: Component of the cytochrome b6-f complex, which mediates
CC electron transfer between photosystem II (PSII) and photosystem I
CC (PSI), cyclic electron flow around PSI, and state transitions.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a plastoquinol + 2 H(+)(in) + 2 oxidized [plastocyanin] = a
CC plastoquinone + 4 H(+)(out) + 2 reduced [plastocyanin];
CC Xref=Rhea:RHEA:22148, Rhea:RHEA-COMP:9561, Rhea:RHEA-COMP:9562,
CC Rhea:RHEA-COMP:10039, Rhea:RHEA-COMP:10040, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17757, ChEBI:CHEBI:29036, ChEBI:CHEBI:49552,
CC ChEBI:CHEBI:62192; EC=7.1.1.6;
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC Evidence={ECO:0000269|PubMed:9438861};
CC Note=Binds 1 [2Fe-2S] cluster per subunit.
CC {ECO:0000269|PubMed:9438861};
CC -!- SUBUNIT: The 4 large subunits of the cytochrome b6-f complex are
CC cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and
CC the Rieske protein, while the 4 small subunits are petG, petL, petM and
CC petN. The complex functions as a dimer (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000305|PubMed:9438861}; Single-pass membrane protein
CC {ECO:0000305|PubMed:9438861}. Note=The transmembrane helix obliquely
CC spans the membrane in one monomer, and its extrinsic C-terminal domain
CC is part of the other monomer. {ECO:0000250}.
CC -!- MISCELLANEOUS: This protein is 1 of 2 subunits of the cytochrome b6-f
CC complex that are encoded in the nucleus.
CC -!- MISCELLANEOUS: The Rieske iron-sulfur protein is a high potential 2Fe-
CC 2S protein.
CC -!- SIMILARITY: Belongs to the Rieske iron-sulfur protein family.
CC {ECO:0000255|HAMAP-Rule:MF_01335}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA29590.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; X06244; CAA29590.1; ALT_INIT; mRNA.
DR PIR; S00454; S00454.
DR PDB; 1RFS; X-ray; 1.83 A; A=92-230.
DR PDB; 6RQF; EM; 3.58 A; D/L=52-230.
DR PDBsum; 1RFS; -.
DR PDBsum; 6RQF; -.
DR AlphaFoldDB; P08980; -.
DR SMR; P08980; -.
DR IntAct; P08980; 1.
DR PRIDE; P08980; -.
DR EvolutionaryTrace; P08980; -.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0045158; F:electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009496; F:plastoquinol--plastocyanin reductase activity; IEA:UniProtKB-EC.
DR Gene3D; 2.102.10.10; -; 1.
DR HAMAP; MF_01335; Cytb6_f_Rieske; 1.
DR InterPro; IPR023960; Cyt_b6_f_Rieske.
DR InterPro; IPR017941; Rieske_2Fe-2S.
DR InterPro; IPR036922; Rieske_2Fe-2S_sf.
DR InterPro; IPR014349; Rieske_Fe-S_prot.
DR InterPro; IPR005805; Rieske_Fe-S_prot_C.
DR PANTHER; PTHR10134; PTHR10134; 1.
DR Pfam; PF00355; Rieske; 1.
DR PRINTS; PR00162; RIESKE.
DR SUPFAM; SSF50022; SSF50022; 1.
DR PROSITE; PS51296; RIESKE; 1.
PE 1: Evidence at protein level;
KW 2Fe-2S; 3D-structure; Chloroplast; Direct protein sequencing;
KW Disulfide bond; Electron transport; Iron; Iron-sulfur; Membrane;
KW Metal-binding; Plastid; Thylakoid; Transit peptide; Translocase;
KW Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..51
FT /note="Chloroplast"
FT /evidence="ECO:0000269|Ref.2"
FT CHAIN 52..230
FT /note="Cytochrome b6-f complex iron-sulfur subunit,
FT chloroplastic"
FT /id="PRO_0000030693"
FT TRANSMEM 73..93
FT /note="Helical"
FT /evidence="ECO:0000305"
FT DOMAIN 116..212
FT /note="Rieske"
FT BINDING 158
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000269|PubMed:9438861"
FT BINDING 160
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000269|PubMed:9438861"
FT BINDING 176
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000269|PubMed:9438861"
FT BINDING 179
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000269|PubMed:9438861"
FT DISULFID 163..178
FT /evidence="ECO:0000269|PubMed:9438861"
FT HELIX 116..122
FT /evidence="ECO:0007829|PDB:1RFS"
FT STRAND 128..132
FT /evidence="ECO:0007829|PDB:1RFS"
FT HELIX 134..136
FT /evidence="ECO:0007829|PDB:1RFS"
FT STRAND 138..143
FT /evidence="ECO:0007829|PDB:1RFS"
FT STRAND 147..149
FT /evidence="ECO:0007829|PDB:1RFS"
FT STRAND 151..155
FT /evidence="ECO:0007829|PDB:1RFS"
FT TURN 159..161
FT /evidence="ECO:0007829|PDB:1RFS"
FT TURN 169..172
FT /evidence="ECO:0007829|PDB:1RFS"
FT STRAND 173..175
FT /evidence="ECO:0007829|PDB:1RFS"
FT TURN 177..179
FT /evidence="ECO:0007829|PDB:1RFS"
FT STRAND 182..184
FT /evidence="ECO:0007829|PDB:1RFS"
FT STRAND 189..193
FT /evidence="ECO:0007829|PDB:1RFS"
FT STRAND 200..206
FT /evidence="ECO:0007829|PDB:1RFS"
FT STRAND 209..214
FT /evidence="ECO:0007829|PDB:1RFS"
FT TURN 220..222
FT /evidence="ECO:0007829|PDB:1RFS"
SQ SEQUENCE 230 AA; 24325 MW; FA2C54B4C6586059 CRC64;
MASFTLSSAT PSQLCSSKNG MFAPSLALAK AGRVNVLISK ERIRGMKLTC QATSIPADNV
PDMQKRETLN LLLLGALSLP TGYMLLPYAS FFVPPGGGAG TGGTIAKDAL GNDVIAAEWL
KTHAPGDRTL TQGLKGDPTY LVVESDKTLA TFGINAVCTH LGCVVPFNAA ENKFICPCHG
SQYNNQGRVV RGPAPLSLAL AHCDVDDGKV VFVPWTETDF RTGEAPWWSA
