UCRIA_WHEAT
ID UCRIA_WHEAT Reviewed; 222 AA.
AC Q7X9A6;
DT 05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Cytochrome b6-f complex iron-sulfur subunit, chloroplastic;
DE EC=7.1.1.6;
DE AltName: Full=Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein;
DE AltName: Full=Rieske iron-sulfur protein;
DE Short=ISP;
DE Short=RISP;
DE Flags: Precursor;
GN Name=petC;
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Lu Z.-X., Laroche A., Gaudet D.;
RT "Triticum aestivum putative Rieske Fe-S precursor protein (RISP).";
RL Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the cytochrome b6-f complex, which mediates
CC electron transfer between photosystem II (PSII) and photosystem I
CC (PSI), cyclic electron flow around PSI, and state transitions.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a plastoquinol + 2 H(+)(in) + 2 oxidized [plastocyanin] = a
CC plastoquinone + 4 H(+)(out) + 2 reduced [plastocyanin];
CC Xref=Rhea:RHEA:22148, Rhea:RHEA-COMP:9561, Rhea:RHEA-COMP:9562,
CC Rhea:RHEA-COMP:10039, Rhea:RHEA-COMP:10040, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17757, ChEBI:CHEBI:29036, ChEBI:CHEBI:49552,
CC ChEBI:CHEBI:62192; EC=7.1.1.6;
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135; Evidence={ECO:0000250};
CC Note=Binds 1 [2Fe-2S] cluster per subunit. {ECO:0000250};
CC -!- SUBUNIT: The 4 large subunits of the cytochrome b6-f complex are
CC cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and
CC the Rieske protein, while the 4 small subunits are petG, petL, petM and
CC petN. The complex functions as a dimer (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000250}; Single-pass membrane protein {ECO:0000250}. Note=The
CC transmembrane helix obliquely spans the membrane in one monomer, and
CC its extrinsic C-terminal domain is part of the other monomer.
CC {ECO:0000250}.
CC -!- MISCELLANEOUS: This protein is 1 of 2 subunits of the cytochrome b6-f
CC complex that are encoded in the nucleus.
CC -!- MISCELLANEOUS: The Rieske iron-sulfur protein is a high potential 2Fe-
CC 2S protein.
CC -!- SIMILARITY: Belongs to the Rieske iron-sulfur protein family.
CC {ECO:0000255|HAMAP-Rule:MF_01335}.
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DR EMBL; AY123422; AAM88439.1; -; mRNA.
DR AlphaFoldDB; Q7X9A6; -.
DR SMR; Q7X9A6; -.
DR STRING; 4565.Traes_2AS_1475F8BDB.1; -.
DR PRIDE; Q7X9A6; -.
DR EnsemblPlants; TraesCAD_scaffold_092989_01G000200.1; TraesCAD_scaffold_092989_01G000200.1; TraesCAD_scaffold_092989_01G000200.
DR EnsemblPlants; TraesCLE_scaffold_085676_01G000200.1; TraesCLE_scaffold_085676_01G000200.1; TraesCLE_scaffold_085676_01G000200.
DR EnsemblPlants; TraesCS2D02G214700.1; TraesCS2D02G214700.1; TraesCS2D02G214700.
DR EnsemblPlants; TraesPAR_scaffold_090294_01G000200.1; TraesPAR_scaffold_090294_01G000200.1; TraesPAR_scaffold_090294_01G000200.
DR EnsemblPlants; TraesROB_scaffold_090335_01G000200.1; TraesROB_scaffold_090335_01G000200.1; TraesROB_scaffold_090335_01G000200.
DR EnsemblPlants; TraesWEE_scaffold_091601_01G000200.1; TraesWEE_scaffold_091601_01G000200.1; TraesWEE_scaffold_091601_01G000200.
DR Gramene; TraesCAD_scaffold_092989_01G000200.1; TraesCAD_scaffold_092989_01G000200.1; TraesCAD_scaffold_092989_01G000200.
DR Gramene; TraesCLE_scaffold_085676_01G000200.1; TraesCLE_scaffold_085676_01G000200.1; TraesCLE_scaffold_085676_01G000200.
DR Gramene; TraesCS2D02G214700.1; TraesCS2D02G214700.1; TraesCS2D02G214700.
DR Gramene; TraesPAR_scaffold_090294_01G000200.1; TraesPAR_scaffold_090294_01G000200.1; TraesPAR_scaffold_090294_01G000200.
DR Gramene; TraesROB_scaffold_090335_01G000200.1; TraesROB_scaffold_090335_01G000200.1; TraesROB_scaffold_090335_01G000200.
DR Gramene; TraesWEE_scaffold_091601_01G000200.1; TraesWEE_scaffold_091601_01G000200.1; TraesWEE_scaffold_091601_01G000200.
DR eggNOG; KOG1671; Eukaryota.
DR OMA; PFNSAEN; -.
DR Proteomes; UP000019116; Unplaced.
DR ExpressionAtlas; Q7X9A6; baseline and differential.
DR GO; GO:0009507; C:chloroplast; IDA:PHI-base.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0045158; F:electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0009496; F:plastoquinol--plastocyanin reductase activity; IDA:PHI-base.
DR GO; GO:0009767; P:photosynthetic electron transport chain; IDA:PHI-base.
DR Gene3D; 2.102.10.10; -; 1.
DR HAMAP; MF_01335; Cytb6_f_Rieske; 1.
DR InterPro; IPR023960; Cyt_b6_f_Rieske.
DR InterPro; IPR017941; Rieske_2Fe-2S.
DR InterPro; IPR036922; Rieske_2Fe-2S_sf.
DR InterPro; IPR014349; Rieske_Fe-S_prot.
DR InterPro; IPR005805; Rieske_Fe-S_prot_C.
DR PANTHER; PTHR10134; PTHR10134; 1.
DR Pfam; PF00355; Rieske; 1.
DR PRINTS; PR00162; RIESKE.
DR SUPFAM; SSF50022; SSF50022; 1.
DR PROSITE; PS51296; RIESKE; 1.
PE 2: Evidence at transcript level;
KW 2Fe-2S; Chloroplast; Disulfide bond; Electron transport; Iron; Iron-sulfur;
KW Membrane; Metal-binding; Plastid; Reference proteome; Thylakoid;
KW Transit peptide; Translocase; Transmembrane; Transmembrane helix;
KW Transport.
FT TRANSIT 1..49
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 50..222
FT /note="Cytochrome b6-f complex iron-sulfur subunit,
FT chloroplastic"
FT /id="PRO_0000030697"
FT TRANSMEM 66..86
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 109..205
FT /note="Rieske"
FT BINDING 151
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000250"
FT BINDING 153
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000250"
FT BINDING 169
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000250"
FT BINDING 172
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000250"
FT DISULFID 156..171
FT /evidence="ECO:0000250"
SQ SEQUENCE 222 AA; 23726 MW; D92CD095FEDFC4D5 CRC64;
MASTALSTAS NPTQLCRTRA SSLCKPVKGL GFGRERIPRN ITCMAGSISA DRVPDMSKRE
LMNLLLLGAI SLPTFGMLVP YGSFLVPAGS GSNAGGVAAK DKLGNDILVE DWLKTHGPND
RTLAQGLKGD PTYLVVESDK TLATYGINAV CTHLGCVVPW NAAENKFLCP CHGSQYNNQG
KVVRGPAPLS LALVHADVDD GKVVFVPWVE TDFRTGDNPW WK
