UCRIB_TOBAC
ID UCRIB_TOBAC Reviewed; 228 AA.
AC Q02585;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Cytochrome b6-f complex iron-sulfur subunit 2, chloroplastic;
DE EC=7.1.1.6;
DE AltName: Full=Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein 2;
DE AltName: Full=Rieske iron-sulfur protein 2;
DE Short=ISP 2;
DE Short=RISP 2;
DE Flags: Precursor;
GN Name=petC2;
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Leaf;
RX PubMed=1391772; DOI=10.1007/bf00014496;
RA Madueo F., Napier J.A., Cejudo F.J., Gray J.C.;
RT "Import and processing of the precursor of the Rieske FeS protein of
RT tobacco chloroplasts.";
RL Plant Mol. Biol. 20:289-299(1992).
CC -!- FUNCTION: Component of the cytochrome b6-f complex, which mediates
CC electron transfer between photosystem II (PSII) and photosystem I
CC (PSI), cyclic electron flow around PSI, and state transitions.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a plastoquinol + 2 H(+)(in) + 2 oxidized [plastocyanin] = a
CC plastoquinone + 4 H(+)(out) + 2 reduced [plastocyanin];
CC Xref=Rhea:RHEA:22148, Rhea:RHEA-COMP:9561, Rhea:RHEA-COMP:9562,
CC Rhea:RHEA-COMP:10039, Rhea:RHEA-COMP:10040, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17757, ChEBI:CHEBI:29036, ChEBI:CHEBI:49552,
CC ChEBI:CHEBI:62192; EC=7.1.1.6;
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135; Evidence={ECO:0000250};
CC Note=Binds 1 [2Fe-2S] cluster per subunit. {ECO:0000250};
CC -!- SUBUNIT: The 4 large subunits of the cytochrome b6-f complex are
CC cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and
CC the Rieske protein, while the 4 small subunits are petG, petL, petM and
CC petN. The complex functions as a dimer (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000250}; Single-pass membrane protein {ECO:0000250}. Note=The
CC transmembrane helix obliquely spans the membrane in one monomer, and
CC its extrinsic C-terminal domain is part of the other monomer.
CC {ECO:0000250}.
CC -!- MISCELLANEOUS: This protein is 1 of 2 subunits of the cytochrome b6-f
CC complex that are encoded in the nucleus.
CC -!- MISCELLANEOUS: The Rieske iron-sulfur protein is a high potential 2Fe-
CC 2S protein.
CC -!- SIMILARITY: Belongs to the Rieske iron-sulfur protein family.
CC {ECO:0000255|HAMAP-Rule:MF_01335}.
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DR EMBL; X66010; CAA46809.1; -; mRNA.
DR RefSeq; NP_001312705.1; NM_001325776.1.
DR AlphaFoldDB; Q02585; -.
DR SMR; Q02585; -.
DR STRING; 4097.Q02585; -.
DR PRIDE; Q02585; -.
DR GeneID; 107805497; -.
DR KEGG; nta:107805497; -.
DR OMA; GTHTHIA; -.
DR PhylomeDB; Q02585; -.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0045158; F:electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0009496; F:plastoquinol--plastocyanin reductase activity; IEA:UniProtKB-EC.
DR Gene3D; 2.102.10.10; -; 1.
DR HAMAP; MF_01335; Cytb6_f_Rieske; 1.
DR InterPro; IPR023960; Cyt_b6_f_Rieske.
DR InterPro; IPR017941; Rieske_2Fe-2S.
DR InterPro; IPR036922; Rieske_2Fe-2S_sf.
DR InterPro; IPR014349; Rieske_Fe-S_prot.
DR InterPro; IPR005805; Rieske_Fe-S_prot_C.
DR PANTHER; PTHR10134; PTHR10134; 1.
DR Pfam; PF00355; Rieske; 1.
DR PRINTS; PR00162; RIESKE.
DR SUPFAM; SSF50022; SSF50022; 1.
DR PROSITE; PS51296; RIESKE; 1.
PE 2: Evidence at transcript level;
KW 2Fe-2S; Chloroplast; Disulfide bond; Electron transport; Iron; Iron-sulfur;
KW Membrane; Metal-binding; Plastid; Reference proteome; Thylakoid;
KW Transit peptide; Translocase; Transmembrane; Transmembrane helix;
KW Transport.
FT TRANSIT 1..49
FT /note="Chloroplast"
FT CHAIN 50..228
FT /note="Cytochrome b6-f complex iron-sulfur subunit 2,
FT chloroplastic"
FT /id="PRO_0000030695"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 115..211
FT /note="Rieske"
FT BINDING 157
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000250"
FT BINDING 159
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000250"
FT BINDING 175
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000250"
FT BINDING 178
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000250"
FT DISULFID 162..177
FT /evidence="ECO:0000250"
SQ SEQUENCE 228 AA; 24108 MW; 0803D582FCD06560 CRC64;
MASSTLSPVT QLCSSKSGLS SVSQCLLLKP MKINSHGLGK DKRMKVKCMA TSIPADDRVP
DMEKRNLMNL LLLGALSLPT AGMLVPYATF FAPPGSGGGS GGTPAKDALG NDVIASEWLK
THPPGNRTLT QGLKGDPTYL VVENDGTLAT YGINAVCTHL GCVVPFNAAE NKFICPCHGS
QYNNQGRVVR GPAPLSLALA HADIDDGKVV FVPWVETDFR TGEAPWWA
