UCRI_THEVB
ID UCRI_THEVB Reviewed; 180 AA.
AC P0C8N8; Q79V50; Q9X9T2;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Cytochrome b6-f complex iron-sulfur subunit {ECO:0000255|HAMAP-Rule:MF_01335};
DE EC=7.1.1.6 {ECO:0000255|HAMAP-Rule:MF_01335};
DE AltName: Full=Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein {ECO:0000255|HAMAP-Rule:MF_01335};
DE Short=ISP {ECO:0000255|HAMAP-Rule:MF_01335};
DE Short=RISP {ECO:0000255|HAMAP-Rule:MF_01335};
DE AltName: Full=Rieske iron-sulfur protein {ECO:0000255|HAMAP-Rule:MF_01335};
GN Name=petC {ECO:0000255|HAMAP-Rule:MF_01335}; OrderedLocusNames=tlr0959;
OS Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1).
OC Bacteria; Cyanobacteria; Pseudanabaenales; Thermosynechococcaceae;
OC Thermosynechococcus.
OX NCBI_TaxID=197221;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NIES-2133 / IAM M-273 / BP-1;
RX PubMed=12240834; DOI=10.1093/dnares/9.4.123;
RA Nakamura Y., Kaneko T., Sato S., Ikeuchi M., Katoh H., Sasamoto S.,
RA Watanabe A., Iriguchi M., Kawashima K., Kimura T., Kishida Y., Kiyokawa C.,
RA Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Sugimoto M.,
RA Takeuchi C., Yamada M., Tabata S.;
RT "Complete genome structure of the thermophilic cyanobacterium
RT Thermosynechococcus elongatus BP-1.";
RL DNA Res. 9:123-130(2002).
CC -!- FUNCTION: Component of the cytochrome b6-f complex, which mediates
CC electron transfer between photosystem II (PSII) and photosystem I
CC (PSI), cyclic electron flow around PSI, and state transitions.
CC {ECO:0000255|HAMAP-Rule:MF_01335}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a plastoquinol + 2 H(+)(in) + 2 oxidized [plastocyanin] = a
CC plastoquinone + 4 H(+)(out) + 2 reduced [plastocyanin];
CC Xref=Rhea:RHEA:22148, Rhea:RHEA-COMP:9561, Rhea:RHEA-COMP:9562,
CC Rhea:RHEA-COMP:10039, Rhea:RHEA-COMP:10040, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17757, ChEBI:CHEBI:29036, ChEBI:CHEBI:49552,
CC ChEBI:CHEBI:62192; EC=7.1.1.6; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01335};
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01335};
CC Note=Binds 1 [2Fe-2S] cluster per subunit. {ECO:0000255|HAMAP-
CC Rule:MF_01335};
CC -!- SUBUNIT: The 4 large subunits of the cytochrome b6-f complex are
CC cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and
CC the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and
CC PetN. The complex functions as a dimer. {ECO:0000255|HAMAP-
CC Rule:MF_01335}.
CC -!- SUBCELLULAR LOCATION: Cellular thylakoid membrane {ECO:0000255|HAMAP-
CC Rule:MF_01335}; Single-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01335}. Note=The transmembrane helix obliquely spans the
CC membrane in one monomer, and its extrinsic C-terminal domain is part of
CC the other monomer. {ECO:0000255|HAMAP-Rule:MF_01335}.
CC -!- MISCELLANEOUS: The Rieske iron-sulfur protein is a high potential 2Fe-
CC 2S protein.
CC -!- SIMILARITY: Belongs to the Rieske iron-sulfur protein family.
CC {ECO:0000255|HAMAP-Rule:MF_01335}.
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DR EMBL; BA000039; BAC08511.1; -; Genomic_DNA.
DR RefSeq; NP_681749.1; NC_004113.1.
DR RefSeq; WP_011056803.1; NC_004113.1.
DR PDB; 3AZC; X-ray; 2.00 A; A=54-180.
DR PDBsum; 3AZC; -.
DR AlphaFoldDB; P0C8N8; -.
DR SMR; P0C8N8; -.
DR STRING; 197221.22294682; -.
DR EnsemblBacteria; BAC08511; BAC08511; BAC08511.
DR KEGG; tel:tlr0959; -.
DR PATRIC; fig|197221.4.peg.1006; -.
DR eggNOG; COG0723; Bacteria.
DR OMA; KGDPTYI; -.
DR OrthoDB; 1632945at2; -.
DR Proteomes; UP000000440; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031676; C:plasma membrane-derived thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0045158; F:electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009496; F:plastoquinol--plastocyanin reductase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-UniRule.
DR Gene3D; 2.102.10.10; -; 1.
DR HAMAP; MF_01335; Cytb6_f_Rieske; 1.
DR InterPro; IPR023960; Cyt_b6_f_Rieske.
DR InterPro; IPR017941; Rieske_2Fe-2S.
DR InterPro; IPR036922; Rieske_2Fe-2S_sf.
DR InterPro; IPR014349; Rieske_Fe-S_prot.
DR InterPro; IPR005805; Rieske_Fe-S_prot_C.
DR PANTHER; PTHR10134; PTHR10134; 1.
DR Pfam; PF00355; Rieske; 1.
DR PRINTS; PR00162; RIESKE.
DR SUPFAM; SSF50022; SSF50022; 1.
DR PROSITE; PS51296; RIESKE; 1.
PE 1: Evidence at protein level;
KW 2Fe-2S; 3D-structure; Disulfide bond; Electron transport; Iron;
KW Iron-sulfur; Membrane; Metal-binding; Reference proteome; Thylakoid;
KW Translocase; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..180
FT /note="Cytochrome b6-f complex iron-sulfur subunit"
FT /id="PRO_0000361773"
FT TRANSMEM 21..43
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01335"
FT DOMAIN 66..162
FT /note="Rieske"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01335"
FT BINDING 108
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01335"
FT BINDING 110
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01335"
FT BINDING 126
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01335"
FT BINDING 129
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01335"
FT DISULFID 113..128
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01335"
FT HELIX 66..70
FT /evidence="ECO:0007829|PDB:3AZC"
FT STRAND 78..82
FT /evidence="ECO:0007829|PDB:3AZC"
FT HELIX 84..86
FT /evidence="ECO:0007829|PDB:3AZC"
FT STRAND 88..93
FT /evidence="ECO:0007829|PDB:3AZC"
FT STRAND 97..99
FT /evidence="ECO:0007829|PDB:3AZC"
FT STRAND 101..105
FT /evidence="ECO:0007829|PDB:3AZC"
FT TURN 109..111
FT /evidence="ECO:0007829|PDB:3AZC"
FT TURN 119..122
FT /evidence="ECO:0007829|PDB:3AZC"
FT STRAND 123..125
FT /evidence="ECO:0007829|PDB:3AZC"
FT TURN 127..129
FT /evidence="ECO:0007829|PDB:3AZC"
FT STRAND 139..143
FT /evidence="ECO:0007829|PDB:3AZC"
FT STRAND 150..155
FT /evidence="ECO:0007829|PDB:3AZC"
FT STRAND 161..165
FT /evidence="ECO:0007829|PDB:3AZC"
FT TURN 171..173
FT /evidence="ECO:0007829|PDB:3AZC"
SQ SEQUENCE 180 AA; 19316 MW; EBB4352DB5DAB6B7 CRC64;
MAQVSGMSDV PDMGRRQFMN LLTFGTITGT ALGALYPVVK YFIPPASGGT GGGAVAKDAL
GNDIKVSEYL AKHLPGDRSL AQGIKGDPTY VIVTEDHQIA NYGLNAVCTH LGCVVPWNVS
ENKFICPCHG SQYDSTGKVV RGPAPLSLAL VKATVTEDDK LVFTPWTEID FRTGKEPWWT
