UD2A3_MOUSE
ID UD2A3_MOUSE Reviewed; 534 AA.
AC Q8BWQ1; Q8R129; Q9D811;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=UDP-glucuronosyltransferase 2A3;
DE Short=UDPGT 2A3;
DE EC=2.4.1.17;
DE Flags: Precursor;
GN Name=Ugt2a3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Liver, and Small intestine;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=17050650; DOI=10.1124/dmd.106.012070;
RA Buckley D.B., Klaassen C.D.;
RT "Tissue- and gender-specific mRNA expression of UDP-
RT glucuronosyltransferases (UGTs) in mice.";
RL Drug Metab. Dispos. 35:121-127(2007).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-135, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=23806337; DOI=10.1016/j.molcel.2013.06.001;
RA Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y.,
RA Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.;
RT "SIRT5-mediated lysine desuccinylation impacts diverse metabolic
RT pathways.";
RL Mol. Cell 50:919-930(2013).
CC -!- FUNCTION: UDP-glucuronosyltransferases catalyze phase II
CC biotransformation reactions in which lipophilic substrates are
CC conjugated with glucuronic acid to increase water solubility and
CC enhance excretion. They are of major importance in the conjugation and
CC subsequent elimination of potentially toxic xenobiotics and endogenous
CC compounds (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor
CC beta-D-glucuronoside + H(+) + UDP; Xref=Rhea:RHEA:21032,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58052, ChEBI:CHEBI:58223,
CC ChEBI:CHEBI:132367, ChEBI:CHEBI:132368; EC=2.4.1.17;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Highly expressed in liver, with lower levels in
CC duodenum and jejunum. {ECO:0000269|PubMed:17050650}.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AK008601; BAB25770.1; -; mRNA.
DR EMBL; AK050327; BAC34191.1; -; mRNA.
DR EMBL; BC025795; AAH25795.1; -; mRNA.
DR CCDS; CCDS19387.1; -.
DR RefSeq; NP_082370.2; NM_028094.3.
DR AlphaFoldDB; Q8BWQ1; -.
DR SMR; Q8BWQ1; -.
DR BioGRID; 215148; 1.
DR STRING; 10090.ENSMUSP00000031195; -.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR GlyGen; Q8BWQ1; 2 sites.
DR iPTMnet; Q8BWQ1; -.
DR PhosphoSitePlus; Q8BWQ1; -.
DR SwissPalm; Q8BWQ1; -.
DR jPOST; Q8BWQ1; -.
DR MaxQB; Q8BWQ1; -.
DR PaxDb; Q8BWQ1; -.
DR PeptideAtlas; Q8BWQ1; -.
DR PRIDE; Q8BWQ1; -.
DR ProteomicsDB; 297801; -.
DR Antibodypedia; 24205; 130 antibodies from 19 providers.
DR DNASU; 72094; -.
DR Ensembl; ENSMUST00000031195; ENSMUSP00000031195; ENSMUSG00000035780.
DR GeneID; 72094; -.
DR KEGG; mmu:72094; -.
DR UCSC; uc008xyh.2; mouse.
DR CTD; 79799; -.
DR MGI; MGI:1919344; Ugt2a3.
DR VEuPathDB; HostDB:ENSMUSG00000035780; -.
DR eggNOG; KOG1192; Eukaryota.
DR GeneTree; ENSGT00940000162432; -.
DR HOGENOM; CLU_012949_0_2_1; -.
DR InParanoid; Q8BWQ1; -.
DR OMA; MVGVPIF; -.
DR OrthoDB; 508327at2759; -.
DR PhylomeDB; Q8BWQ1; -.
DR TreeFam; TF315472; -.
DR BioGRID-ORCS; 72094; 3 hits in 70 CRISPR screens.
DR ChiTaRS; Ugt2a3; mouse.
DR PRO; PR:Q8BWQ1; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q8BWQ1; protein.
DR Bgee; ENSMUSG00000035780; Expressed in left lobe of liver and 40 other tissues.
DR Genevisible; Q8BWQ1; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015020; F:glucuronosyltransferase activity; ISO:MGI.
DR GO; GO:0052695; P:cellular glucuronidation; ISO:MGI.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Glycosyltransferase; Membrane; Reference proteome; Signal;
KW Transferase; Transmembrane; Transmembrane helix.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..534
FT /note="UDP-glucuronosyltransferase 2A3"
FT /id="PRO_0000299147"
FT TOPO_DOM 19..493
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 494..514
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 515..534
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 135
FT /note="N6-succinyllysine"
FT /evidence="ECO:0007744|PubMed:23806337"
FT CARBOHYD 102
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 204
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 252
FT /note="K -> E (in Ref. 1; BAB25770)"
FT /evidence="ECO:0000305"
FT CONFLICT 284
FT /note="P -> S (in Ref. 2; AAH25795)"
FT /evidence="ECO:0000305"
FT CONFLICT 346
FT /note="T -> I (in Ref. 1; BAB25770)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 534 AA; 61119 MW; 3BF4F591395B1620 CRC64;
MVSEKCVAAF FLLQLCWAGC GFCSKVLVWP CDMSHWLNLK TILEELGARG HEVTVLKYPS
IIIDQSKRIP LHFENIPLLY EIETAENRLN EIANLAVNVI PNLSLWEAAK TLQDFFLQVT
GDFESICRSV LYNQKFMDKL RDAQYDVVVI DPVVPCGELV AEVLQIPFVY TLRFSMGYYM
EKHCGQLPIP LSYVPVVMSE LTDNMTFTER VKNMMFSLLF EYWLQQYDFA FWDQFYSETL
GRPTTFCKTV GKADIWLIRT YWDVEFPRPY LPNFEFVGGL HCKPAKPLPK EMEEFVQSSG
EHGVVVFSLG SMVKNLTEEK ANLIASVLAQ IPQKVLWRYS GKKPATLGSN TRLFNWIPQN
DLLGHPKTKA FITHGGTNGI YEAIYHGVPM VGVPMLGDQP HNIAHMEAKG AALKVSISTM
TSTDLLSAVR AVINEPSYKE NAMRLSRIHH DQPVKPLDRA VFWIEFVMRH KGAKHLRVAA
HDLSWFQYHS LDVIGFLLLC VVTLTFIITK FCLFVCQKLY MKESKKMGNR KKKN
