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UD2B2_RAT
ID   UD2B2_RAT               Reviewed;         530 AA.
AC   P08541;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1988, sequence version 1.
DT   03-AUG-2022, entry version 138.
DE   RecName: Full=UDP-glucuronosyltransferase 2B2;
DE            Short=UDPGT 2B2;
DE            EC=2.4.1.17 {ECO:0000269|PubMed:2429951};
DE   AltName: Full=3-hydroxyandrogen-specific UDPGT;
DE   AltName: Full=RLUG23;
DE   AltName: Full=UDPGTr-4 {ECO:0000303|PubMed:2429951};
DE   Flags: Precursor;
GN   Name=Ugt2b; Synonyms=Ugt2b2;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CATALYTIC ACTIVITY.
RC   TISSUE=Liver;
RX   PubMed=2429951; DOI=10.1016/s0021-9258(18)66989-3;
RA   McKenzie P.I.;
RT   "Rat liver UDP-glucuronosyltransferase. cDNA sequence and expression of a
RT   form glucuronidating 3-hydroxyandrogens.";
RL   J. Biol. Chem. 261:14112-14117(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1909872; DOI=10.1089/dna.1991.10.515;
RA   Haque S.J., Peterson D.D., Nebert D.W., McKenzie P.I.;
RT   "Isolation, sequence, and developmental expression of rat UGT2B2: the gene
RT   encoding a constitutive UDP glucuronosyltransferase that metabolizes
RT   etiocholanolone and androsterone.";
RL   DNA Cell Biol. 10:515-524(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 30-530.
RC   TISSUE=Liver;
RX   PubMed=3003696; DOI=10.1093/nar/14.2.779;
RA   Jackson M.R., Burchell B.;
RT   "The full length coding sequence of rat liver androsterone UDP-
RT   glucuronyltransferase cDNA and comparison with other members of this gene
RT   family.";
RL   Nucleic Acids Res. 14:779-795(1986).
RN   [4]
RP   PROTEIN SEQUENCE OF 24-44, AND LIPID-BINDING.
RX   PubMed=7492328; DOI=10.1042/bj3120301;
RA   Yamashita A., Watanabe M., Tonegawa T., Sugiura T., Waku K.;
RT   "Acyl-CoA binding and acylation of UDP-glucuronosyltransferase isoforms of
RT   rat liver: their effect on enzyme activity.";
RL   Biochem. J. 312:301-308(1995).
CC   -!- FUNCTION: UDPGT is of major importance in the conjugation and
CC       subsequent elimination of potentially toxic xenobiotics and endogenous
CC       compounds. 2B2 acts on various endogenous steroids, especially
CC       etiocholanolone and androsterone.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor
CC         beta-D-glucuronoside + H(+) + UDP; Xref=Rhea:RHEA:21032,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:58052, ChEBI:CHEBI:58223,
CC         ChEBI:CHEBI:132367, ChEBI:CHEBI:132368; EC=2.4.1.17;
CC         Evidence={ECO:0000269|PubMed:2429951};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:21033;
CC         Evidence={ECO:0000305|PubMed:2429951};
CC   -!- SUBCELLULAR LOCATION: Microsome membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000305}. Endoplasmic reticulum membrane
CC       {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.
CC   -!- INDUCTION: Constitutively expressed.
CC   -!- PTM: Autoacylation in vitro.
CC   -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; J02589; AAA42314.1; -; Genomic_DNA.
DR   EMBL; M74439; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; X03478; CAA27198.1; -; mRNA.
DR   PIR; A40467; A40467.
DR   AlphaFoldDB; P08541; -.
DR   SMR; P08541; -.
DR   IntAct; P08541; 2.
DR   STRING; 10116.ENSRNOP00000043239; -.
DR   CAZy; GT1; Glycosyltransferase Family 1.
DR   GlyGen; P08541; 1 site.
DR   iPTMnet; P08541; -.
DR   PhosphoSitePlus; P08541; -.
DR   PaxDb; P08541; -.
DR   UCSC; RGD:3936; rat.
DR   RGD; 3936; Ugt2b.
DR   eggNOG; KOG1192; Eukaryota.
DR   InParanoid; P08541; -.
DR   PhylomeDB; P08541; -.
DR   BRENDA; 2.4.1.17; 5301.
DR   Reactome; R-RNO-156588; Glucuronidation.
DR   Reactome; R-RNO-9749641; Aspirin ADME.
DR   Reactome; R-RNO-9753281; Paracetamol ADME.
DR   PRO; PR:P08541; -.
DR   Proteomes; UP000002494; Unplaced.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015020; F:glucuronosyltransferase activity; IDA:RGD.
DR   GO; GO:0052695; P:cellular glucuronidation; IBA:GO_Central.
DR   GO; GO:0008210; P:estrogen metabolic process; IBA:GO_Central.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   InterPro; IPR035595; UDP_glycos_trans_CS.
DR   Pfam; PF00201; UDPGT; 1.
DR   PROSITE; PS00375; UDPGT; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Endoplasmic reticulum; Glycoprotein;
KW   Glycosyltransferase; Membrane; Microsome; Reference proteome; Signal;
KW   Transferase; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000269|PubMed:7492328"
FT   CHAIN           24..530
FT                   /note="UDP-glucuronosyltransferase 2B2"
FT                   /id="PRO_0000036026"
FT   TRANSMEM        494..510
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        316
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        159
FT                   /note="D -> E (in Ref. 3; CAA27198)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        286
FT                   /note="A -> S (in Ref. 3; CAA27198)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        351
FT                   /note="N -> I (in Ref. 3; CAA27198)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        363
FT                   /note="L -> I (in Ref. 3; CAA27198)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   530 AA;  60986 MW;  F2FFF3E23E2D75B2 CRC64;
     MPRKWISALF LLQISYCFKS GHCGKVLVWP MDFSHWMNIK IILDELVQRG HEVTVLKPSA
     YFFLDPKKSS DLKFEIFSTS ISKDELQNHF IKLLDVWTYE LPRDTCLSYS PILQNLVYEF
     SYFYLSICKD AVSNKQLMTK LQESKFDVLF ADPVASCGDL IAELLHIPFL YSLSFSPGHK
     LEKSIGKFIL PPSYVPVILS GLAGKMTFID RVKNMICMLY FDFWFERLRH KEWDTFYSEI
     LGRPTTVDET MSKVEIWLIR SYWDLKFPHP TLPNVDYIGG LHCKPAKPLP KDMEEFVQSS
     GEHGVVVFSL GSMVSNMTEE KANAIAWALA QIPQKVLWKF DGKTPATLGP NTRVYKWLPQ
     NDLLGHPKTK AFVTHGGANG LYEAIYHGIP MIGIPLFGDQ PDNIAHMVAK GAAVSLNIRT
     MSKLDFLSAL EEVIDNPFYK KNVMLLSTIH HDQPMKPLDR AVFWIEFIMR HKGAKHLRPL
     GHNLPWYQYH SLDVIGFLLT CFAVIAALTV KCLLFMYRFF VKKEKKMKNE
 
 
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