UD3A2_MOUSE
ID UD3A2_MOUSE Reviewed; 523 AA.
AC Q8JZZ0; A1A4B4; Q3UP49; Q8VC11;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-AUG-2007, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=UDP-glucuronosyltransferase 3A2;
DE Short=UDPGT 3A2;
DE EC=2.4.1.17;
DE Flags: Precursor;
GN Name=Ugt3a2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Liver, and Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Kidney, and Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=17050650; DOI=10.1124/dmd.106.012070;
RA Buckley D.B., Klaassen C.D.;
RT "Tissue- and gender-specific mRNA expression of UDP-
RT glucuronosyltransferases (UGTs) in mice.";
RL Drug Metab. Dispos. 35:121-127(2007).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, and Liver;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: UDP-glucuronosyltransferases catalyze phase II
CC biotransformation reactions in which lipophilic substrates are
CC conjugated with glucuronic acid to increase water solubility and
CC enhance excretion. They are of major importance in the conjugation and
CC subsequent elimination of potentially toxic xenobiotics and endogenous
CC compounds (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor
CC beta-D-glucuronoside + H(+) + UDP; Xref=Rhea:RHEA:21032,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58052, ChEBI:CHEBI:58223,
CC ChEBI:CHEBI:132367, ChEBI:CHEBI:132368; EC=2.4.1.17;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Highly expressed in kidney, while it is expressed
CC at low levels in liver. Not detected in other tissues examined.
CC {ECO:0000269|PubMed:17050650}.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AK050128; BAC34080.1; -; mRNA.
DR EMBL; AK143815; BAE25548.1; -; mRNA.
DR EMBL; BC022134; AAH22134.1; -; mRNA.
DR EMBL; BC024453; AAH24453.1; -; mRNA.
DR EMBL; BC034837; AAH34837.1; -; mRNA.
DR CCDS; CCDS27375.1; -.
DR RefSeq; NP_659094.1; NM_144845.3.
DR AlphaFoldDB; Q8JZZ0; -.
DR SMR; Q8JZZ0; -.
DR STRING; 10090.ENSMUSP00000072236; -.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR GlyGen; Q8JZZ0; 1 site.
DR iPTMnet; Q8JZZ0; -.
DR PhosphoSitePlus; Q8JZZ0; -.
DR jPOST; Q8JZZ0; -.
DR MaxQB; Q8JZZ0; -.
DR PaxDb; Q8JZZ0; -.
DR PeptideAtlas; Q8JZZ0; -.
DR PRIDE; Q8JZZ0; -.
DR ProteomicsDB; 297803; -.
DR DNASU; 223337; -.
DR Ensembl; ENSMUST00000072403; ENSMUSP00000072236; ENSMUSG00000049152.
DR GeneID; 223337; -.
DR KEGG; mmu:223337; -.
DR UCSC; uc007vfl.1; mouse.
DR CTD; 167127; -.
DR MGI; MGI:2145969; Ugt3a2.
DR VEuPathDB; HostDB:ENSMUSG00000049152; -.
DR eggNOG; KOG1192; Eukaryota.
DR GeneTree; ENSGT00940000161263; -.
DR HOGENOM; CLU_012949_3_2_1; -.
DR InParanoid; Q8JZZ0; -.
DR OMA; YASFQRP; -.
DR OrthoDB; 508327at2759; -.
DR PhylomeDB; Q8JZZ0; -.
DR TreeFam; TF315472; -.
DR Reactome; R-MMU-156588; Glucuronidation.
DR Reactome; R-MMU-9749641; Aspirin ADME.
DR BioGRID-ORCS; 223337; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Ugt3a2; mouse.
DR PRO; PR:Q8JZZ0; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q8JZZ0; protein.
DR Bgee; ENSMUSG00000049152; Expressed in right kidney and 26 other tissues.
DR Genevisible; Q8JZZ0; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043541; C:UDP-N-acetylglucosamine transferase complex; IBA:GO_Central.
DR GO; GO:0015020; F:glucuronosyltransferase activity; IBA:GO_Central.
DR GO; GO:0008194; F:UDP-glycosyltransferase activity; IDA:MGI.
DR GO; GO:0071412; P:cellular response to genistein; ISO:MGI.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Glycosyltransferase; Membrane; Reference proteome; Signal;
KW Transferase; Transmembrane; Transmembrane helix.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..523
FT /note="UDP-glucuronosyltransferase 3A2"
FT /id="PRO_0000299154"
FT TOPO_DOM 23..487
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 488..508
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 509..523
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 52
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 121
FT /note="S -> G (in Ref. 1; BAE25548)"
FT /evidence="ECO:0000305"
FT CONFLICT 344
FT /note="P -> S (in Ref. 2; AAH34837)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 523 AA; 59673 MW; BC7BD6ADF197ADD9 CRC64;
MAAHRRWLLM SFLFLEVILL EAAKILTIST LSASHYIVIS RVSQVLHEGG HNVTKLLYES
ANIPDFRKEK PSYQVINWRP PEDQEKKFAD LRHRLTEEIT YGRSKHHTLL KIHQYFGDLC
SQLLSRKDIM DFLKNENFDL VLLDSMDLCS LLIVEKLGKR FVSFLPFQFS YMDFGLPSAP
LSYAPVYGSG LTDQMDFWGR VKNFLMFLDF SMKQREILSQ YDSTIQEHFV EGSQPVLSDL
LLKAELWFVN SDFALDFARP LFPNTVYVGG LLDKPVQPIP QDLENFISQF GDSGFVLVAL
GSIVSMIQSK EIIKEMNSAF AHLPQGVLWT CKTSHWPKDV SLAPNVKIMD WLPQTDLLAH
PSIRLFVTHG GMNSVMEAVH HGVPMVGIPF FFDQPENMVR VEAKNLGVSI QLQTLKAESF
ALTMKKIIED KRYKSAAMAS KIIRHSHPLT PAQRLLGWID HILQTGGAAH LKPYAFQQPW
HEQYMLDVFL FLLGLMLGTL WLSVKVLVAV TRYLSIATKV KEA
